BRENDA - Enzyme Database
show all sequences of 1.4.3.10

Mechanism of action of putrescine oxidase. Binding characteristics of the active site of putrescine oxidase from Micrococcus rubens

Swain, W.F.; Desa, R.J.; Biochim. Biophys. Acta 429, 331-341 (1976)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
1,1,4,4-tetramethyl-1,4-diaminobutane
-
Kocuria rosea
1,10-Diaminodecane
-
Kocuria rosea
1,12-diaminododecane
-
Kocuria rosea
1,3-Diaminopropane
-
Kocuria rosea
1,6-diaminohexane
-
Kocuria rosea
1,7-Diaminoheptane
-
Kocuria rosea
1,8-diaminooctane
-
Kocuria rosea
1-aminoethanol
-
Kocuria rosea
2-Aminoethanol
-
Kocuria rosea
3-Amino-1-propanol
-
Kocuria rosea
4-amino-1-butanoic acid
-
Kocuria rosea
5-amino-1-pentanoic acid
-
Kocuria rosea
5-amino-1-pentanol
-
Kocuria rosea
6-amino-1-hexanoic acid
-
Kocuria rosea
6-amino-1-hexanol
-
Kocuria rosea
8-amino-1-octanoic acid
-
Kocuria rosea
agmatine
-
Kocuria rosea
allylamine
-
Kocuria rosea
ammonium
-
Kocuria rosea
benzylamine
-
Kocuria rosea
Butylamine
-
Kocuria rosea
dodecylamine
-
Kocuria rosea
ethylamine
-
Kocuria rosea
ethylenediamine
-
Kocuria rosea
heptylamine
-
Kocuria rosea
Hexylamine
-
Kocuria rosea
methylamine
-
Kocuria rosea
N,N,N',N'-tetramethyl-1,4-diaminobutane
-
Kocuria rosea
pentylamine
-
Kocuria rosea
Phenylethylamine
-
Kocuria rosea
Propylamine
-
Kocuria rosea
tyramine
-
Kocuria rosea
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Kocuria rosea
-
-
-
Reaction
Reaction
Commentary
Organism
putrescine + O2 + H2O = 4-aminobutanal + NH3 + H2O2
mechanism
Kocuria rosea
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1,6-diaminohexane + O2 + H2O
1% of the activity with putrescine
395447
Kocuria rosea
?
-
-
-
?
cadaverine + O2 + H2O
7% of the activity with putrescine
395447
Kocuria rosea
5-aminopentanal + NH3 + H2O2
-
-
-
?
putrescine + O2 + H2O
-
395447
Kocuria rosea
4-aminobutanal + NH3 + H2O2
-
-
-
?
spermidine + O2 + H2O
10% of the activity with putrescine
395447
Kocuria rosea
4-aminobutyraldehyde + 1,3-diaminopropane + H2O2
-
-
-
?
Ki Value [mM]
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.002
-
1,12-diaminododecane
-
Kocuria rosea
0.003
-
1,10-Diaminodecane
-
Kocuria rosea
0.0032
-
1,8-diaminooctane
-
Kocuria rosea
0.0082
-
1,7-Diaminoheptane
-
Kocuria rosea
0.0082
-
dodecylamine
-
Kocuria rosea
0.013
-
allylamine
-
Kocuria rosea
0.014
-
heptylamine
-
Kocuria rosea
0.023
-
1,6-diaminohexane
-
Kocuria rosea
0.036
-
agmatine
-
Kocuria rosea
0.039
-
Hexylamine
-
Kocuria rosea
0.039
-
tyramine
-
Kocuria rosea
0.047
-
benzylamine
-
Kocuria rosea
0.087
-
Phenylethylamine
-
Kocuria rosea
0.088
-
5-amino-1-pentanol
-
Kocuria rosea
0.12
-
1,3-Diaminopropane
-
Kocuria rosea
0.14
-
N,N,N',N'-tetramethyl-1,4-diaminobutane
-
Kocuria rosea
0.19
-
1,1,4,4-tetramethyl-1,4-diaminobutane
-
Kocuria rosea
0.24
-
pentylamine
-
Kocuria rosea
0.31
-
6-amino-1-hexanol
-
Kocuria rosea
0.46
-
Butylamine
-
Kocuria rosea
0.5
-
3-Amino-1-propanol
-
Kocuria rosea
0.62
-
Propylamine
-
Kocuria rosea
0.67
-
3-Amino-1-propanol
-
Kocuria rosea
0.95
-
ethylenediamine
-
Kocuria rosea
2.3
-
ethylamine
-
Kocuria rosea
3
-
2-Aminoethanol
-
Kocuria rosea
3.4
-
methylamine
-
Kocuria rosea
20
-
ammonium
-
Kocuria rosea
59
-
5-amino-1-pentanoic acid
-
Kocuria rosea
160
-
8-amino-1-octanoic acid
-
Kocuria rosea
300
-
6-amino-1-hexanoic acid
-
Kocuria rosea
440
-
4-amino-1-butanoic acid
-
Kocuria rosea
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
1,1,4,4-tetramethyl-1,4-diaminobutane
-
Kocuria rosea
1,10-Diaminodecane
-
Kocuria rosea
1,12-diaminododecane
-
Kocuria rosea
1,3-Diaminopropane
-
Kocuria rosea
1,6-diaminohexane
-
Kocuria rosea
1,7-Diaminoheptane
-
Kocuria rosea
1,8-diaminooctane
-
Kocuria rosea
1-aminoethanol
-
Kocuria rosea
2-Aminoethanol
-
Kocuria rosea
3-Amino-1-propanol
-
Kocuria rosea
4-amino-1-butanoic acid
-
Kocuria rosea
5-amino-1-pentanoic acid
-
Kocuria rosea
5-amino-1-pentanol
-
Kocuria rosea
6-amino-1-hexanoic acid
-
Kocuria rosea
6-amino-1-hexanol
-
Kocuria rosea
8-amino-1-octanoic acid
-
Kocuria rosea
agmatine
-
Kocuria rosea
allylamine
-
Kocuria rosea
ammonium
-
Kocuria rosea
benzylamine
-
Kocuria rosea
Butylamine
-
Kocuria rosea
dodecylamine
-
Kocuria rosea
ethylamine
-
Kocuria rosea
ethylenediamine
-
Kocuria rosea
heptylamine
-
Kocuria rosea
Hexylamine
-
Kocuria rosea
methylamine
-
Kocuria rosea
N,N,N',N'-tetramethyl-1,4-diaminobutane
-
Kocuria rosea
pentylamine
-
Kocuria rosea
Phenylethylamine
-
Kocuria rosea
Propylamine
-
Kocuria rosea
tyramine
-
Kocuria rosea
Ki Value [mM] (protein specific)
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.002
-
1,12-diaminododecane
-
Kocuria rosea
0.003
-
1,10-Diaminodecane
-
Kocuria rosea
0.0032
-
1,8-diaminooctane
-
Kocuria rosea
0.0082
-
1,7-Diaminoheptane
-
Kocuria rosea
0.0082
-
dodecylamine
-
Kocuria rosea
0.013
-
allylamine
-
Kocuria rosea
0.014
-
heptylamine
-
Kocuria rosea
0.023
-
1,6-diaminohexane
-
Kocuria rosea
0.036
-
agmatine
-
Kocuria rosea
0.039
-
Hexylamine
-
Kocuria rosea
0.039
-
tyramine
-
Kocuria rosea
0.047
-
benzylamine
-
Kocuria rosea
0.087
-
Phenylethylamine
-
Kocuria rosea
0.088
-
5-amino-1-pentanol
-
Kocuria rosea
0.12
-
1,3-Diaminopropane
-
Kocuria rosea
0.14
-
N,N,N',N'-tetramethyl-1,4-diaminobutane
-
Kocuria rosea
0.19
-
1,1,4,4-tetramethyl-1,4-diaminobutane
-
Kocuria rosea
0.24
-
pentylamine
-
Kocuria rosea
0.31
-
6-amino-1-hexanol
-
Kocuria rosea
0.46
-
Butylamine
-
Kocuria rosea
0.5
-
3-Amino-1-propanol
-
Kocuria rosea
0.62
-
Propylamine
-
Kocuria rosea
0.67
-
3-Amino-1-propanol
-
Kocuria rosea
0.95
-
ethylenediamine
-
Kocuria rosea
2.3
-
ethylamine
-
Kocuria rosea
3
-
2-Aminoethanol
-
Kocuria rosea
3.4
-
methylamine
-
Kocuria rosea
20
-
ammonium
-
Kocuria rosea
59
-
5-amino-1-pentanoic acid
-
Kocuria rosea
160
-
8-amino-1-octanoic acid
-
Kocuria rosea
300
-
6-amino-1-hexanoic acid
-
Kocuria rosea
440
-
4-amino-1-butanoic acid
-
Kocuria rosea
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1,6-diaminohexane + O2 + H2O
1% of the activity with putrescine
395447
Kocuria rosea
?
-
-
-
?
cadaverine + O2 + H2O
7% of the activity with putrescine
395447
Kocuria rosea
5-aminopentanal + NH3 + H2O2
-
-
-
?
putrescine + O2 + H2O
-
395447
Kocuria rosea
4-aminobutanal + NH3 + H2O2
-
-
-
?
spermidine + O2 + H2O
10% of the activity with putrescine
395447
Kocuria rosea
4-aminobutyraldehyde + 1,3-diaminopropane + H2O2
-
-
-
?
Other publictions for EC 1.4.3.10
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
742361
Henao-Escobar
-
Characterization of a disposa ...
Kocuria rosea
Electroanalysis
27
368-377
2015
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742489
Kopacz
Kinetic mechanism of putresci ...
Rhodococcus erythropolis
FEBS J.
281
4384-4393
2014
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725184
Lee
Construction of a bifunctional ...
Paenarthrobacter aurescens, Paenarthrobacter aurescens TC1
J. Agric. Food Chem.
61
9118-9124
2013
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1
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3
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8
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8
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1
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3
1
1
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3
3
726504
Foster
Genomic organisation, activity ...
Rhodococcus opacus, Rhodococcus opacus DSM 42350
Syst. Appl. Microbiol.
36
457-466
2013
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7
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724877
Boka
Putrescine biosensor based on ...
Kocuria rosea, Kocuria rosea K22
Enzyme Microb. Technol.
51
258-262
2012
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1
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1
2
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5
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11
1
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1
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1
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1
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1
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724314
Kopacz
Structure-based redesign of co ...
Rhodococcus erythropolis
Biochemistry
50
4209-4217
2011
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1
1
4
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4
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1
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3
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1
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1
1
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4
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2
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1
2
1
4
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4
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1
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1
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1
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4
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4
4
684634
van Hellemond
Discovery and characterization ...
Rhodococcus erythropolis, Rhodococcus erythropolis NCIMB 11540
Appl. Microbiol. Biotechnol.
78
455-463
2008
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1
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2
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6
9
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3
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5
-
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1
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14
1
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1
10
1
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1
1
5
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1
1
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2
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6
5
9
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3
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1
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14
1
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1
10
1
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1
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687763
van Hellemond
ADP competes with FAD binding ...
Rhodococcus erythropolis, Rhodococcus erythropolis NCIMB 11540
J. Biol. Chem.
283
28259-28264
2008
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1
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4
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654187
Rochette
A mediatorless biosensor for p ...
Kocuria rosea
Anal. Biochem.
336
305-311
2005
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395444
Gunasekaran
Partial purification and prope ...
Candida albicans, Candida parapsilosis, Candida tropicalis, Meyerozyma guilliermondii, Pichia kudriavzevii
Appl. Biochem. Biotechnol.
76
229-236
1999
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3
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1
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7
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1
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9
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1
1
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1
1
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9
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1
1
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1
1
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395445
Perez-Amador
Arginine decarboxylase and put ...
Pisum sativum
Plant Physiol.
107
865-872
1995
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4
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1
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395443
Ishizuka
Putrescine oxidase of Micrococ ...
Kocuria rosea
J. Gen. Microbiol.
139
425-432
1993
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1
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1
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1
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1
1
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288077
Shimizu
-
Specific measurement of putres ...
Pseudomonas putida
Agric. Biol. Chem.
52
2865-2871
1988
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1
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1
1
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1
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1
3
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1
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1
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1
1
3
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3
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1
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1
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395440
Rokka
-
Role of putrescine oxidase in ...
Micrococcus luteus
J. Gen. Appl. Microbiol.
31
435-440
1985
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1
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395436
Okada
Purification of putrescine oxi ...
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1983
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1
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1
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1
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395441
Spathas
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Putrescine as a nitrogen sourc ...
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1983
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1
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Gahl
Polyamine degradation in foeta ...
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1982
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2
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13
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2
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3
2
2
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2
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13
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395446
Isobe
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Differential determination pro ...
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1981
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1
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1
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2
1
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1
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Okada
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1980
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7
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3
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1
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1
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7
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3
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395449
Gahl
Separation of putrescine oxida ...
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1980
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2
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1
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2
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2
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1
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395447
Swain
Mechanism of action of putresc ...
Kocuria rosea
Biochim. Biophys. Acta
429
331-341
1976
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1
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4
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32
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32
32
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4
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395438
Desa
Putrescine oxidase from Microc ...
Kocuria rosea
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247
5527-5534
1972
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2
1
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1
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1
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2
1
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1
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1
1
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1
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1
1
2
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3
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1
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1
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1
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2
1
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1
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395437
Yamada
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Putrescine oxidase (Micrococcu ...
Kocuria rosea
Methods Enzymol.
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726-730
1971
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2
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1
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2
3
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2
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2
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1
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1
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4
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2
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1
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2
3
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2
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2
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395450
Adachi
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Purification and properties of ...
Kocuria rosea
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1202-1210
1966
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1
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1
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1
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1
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1
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1
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1
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1
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