Literature summary for 1.4.3.1 extracted from

Puggioni, V.; Savinelli, A.; Miceli, M.; Molla, G.; Pollegioni, L.; Sacchi, S.
Biochemical characterization of mouse D-aspartate oxidase (2020), Biochim. Biophys. Acta, 1868, 140472 .

Search for more literature for 1.4.3.1

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Mus musculus

Inhibitors

Inhibitors	Comment	Organism	Structure
5-aminonicotinic acid	Kd value 5.7 microM	Mus musculus
pyrido[2,3-b]pyrazine-2,3(1H,4H)-dione	Kd value 19.8 microM	Mus musculus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
2.1	-	D-Aspartate	pH 8.3, 25°C	Mus musculus
4.2	-	N-Methyl-D-aspartate	pH 8.3, 25°C	Mus musculus
8.8	-	D-histidine	pH 8.3, 25°C	Mus musculus
68.4	-	D-glutamate	pH 8.3, 25°C	Mus musculus
101.4	-	D-asparagine	pH 8.3, 25°C	Mus musculus
169	-	D-proline	pH 8.3, 25°C	Mus musculus

Organism

Organism	UniProt	Comment	Textmining
Mus musculus	Q0E9Q7	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant protein, purified to homogeneity from the soluble fraction by HiTrap chelating chromatography	Mus musculus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
D-asparagine + H2O + O2	-	Mus musculus	2-oxosuccinamic acid + H2O2 + NH3	-	?
D-aspartate + H2O + O2	-	Mus musculus	oxaloacetate + H2O2 + NH3	-	?
D-glutamate + H2O + O2	-	Mus musculus	2-oxoglutarate + NH3 + H2O2	-	?
D-histidine + H2O + O2	-	Mus musculus	3-(1H-imidazol-4-yl)-2-oxopropanoic acid + NH3 + H2O2	-	?
D-proline + H2O + O2	-	Mus musculus	? + NH3 + H2O2	-	?
N-methyl-D-aspartate + H2O + O2	-	Mus musculus	oxaloacetate + CH3NH2 + H2O2	-	?

Synonyms

Synonyms	Comment	Organism
DASPO	-	Mus musculus
DDO	-	Mus musculus

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.71	-	D-histidine	pH 8.3, 25°C	Mus musculus
5.5	-	D-proline	pH 8.3, 25°C	Mus musculus
11.4	-	D-asparagine	pH 8.3, 25°C	Mus musculus
16.8	-	D-glutamate	pH 8.3, 25°C	Mus musculus
58.4	-	D-Aspartate	pH 8.3, 25°C	Mus musculus
112.1	-	N-Methyl-D-aspartate	pH 8.3, 25°C	Mus musculus

Cofactor

Cofactor	Comment	Organism	Structure
FAD	protein shows a classical absorbance spectrum of a FAD-containing protein, with absorbance maxima at 449, 361 and 273 nm, and a 273 nm/449 nm absorbance ratio of 10.4	Mus musculus

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
0.03	-	D-proline	pH 8.3, 25°C	Mus musculus
0.08	-	D-histidine	pH 8.3, 25°C	Mus musculus
0.11	-	D-asparagine	pH 8.3, 25°C	Mus musculus
0.25	-	D-glutamate	pH 8.3, 25°C	Mus musculus
26.7	-	N-Methyl-D-aspartate	pH 8.3, 25°C	Mus musculus
27.8	-	D-Aspartate	pH 8.3, 25°C	Mus musculus

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Release 2023.1 (January 2023)