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Literature summary for 1.4.1.9 extracted from

  • Li, J.; Pan, J.; Zhang, J.; Xu, J.
    Stereoselective synthesis of L-tert-leucine by a newly cloned leucine dehydrogenase from Exiguobacterium sibiricum (2014), J. Mol. Catal. B, 105, 11-17 .
No PubMed abstract available

Application

Application Comment Organism
synthesis coexpression with Bacillus megaterium glucose dehydrogenase in Escherichia coli for the production of L-tert-leucine. A decagram preparation of L-tert-leucine is performed at a substrate concentration of 0.6 M in 1 l scale with 99% conversion after 5.5 h, resulting in 80.1% yield and > 99% enantiomeric excess Exiguobacterium sibiricum

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Exiguobacterium sibiricum

Inhibitors

Inhibitors Comment Organism Structure
Ag+ 1 mM, no residual activity Exiguobacterium sibiricum
Cu2+ 1 mM, 58.7% residual activity Exiguobacterium sibiricum
Fe3+ 1 mM, no residual activity Exiguobacterium sibiricum
Mn2+ 1 mM, 32.7% residual activity Exiguobacterium sibiricum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.077
-
NADH pH 9.5, 30°C Exiguobacterium sibiricum
0.49
-
NAD+ pH 9.5, 30°C Exiguobacterium sibiricum
0.8
-
L-leucine pH 9.5, 30°C Exiguobacterium sibiricum
16.8
-
3,3-dimethyl-2-oxobutanoate pH 9.5, 30°C Exiguobacterium sibiricum

Organism

Organism UniProt Comment Textmining
Exiguobacterium sibiricum
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-oxobutanoate + NH3 + NADH + H+
-
Exiguobacterium sibiricum L-2-aminobutanoate + H2O + NAD+
-
r
2-oxophenylacetic acid + NH3 + NADH + H+
-
Exiguobacterium sibiricum 2-aminophenylacetic acid + H2O + NAD+
-
r
3,3-dimethyl-2-oxobutanoate + NH3 + NADH + H+
-
Exiguobacterium sibiricum L-tert-Leu + H2O + NAD+
-
r
3-methyl-2-oxobutanoate + NH3 + NADH + H+
-
Exiguobacterium sibiricum L-valine + H2O + NAD+
-
r
4-methyl-2-oxopentanoate + NH3 + NADH + H+
-
Exiguobacterium sibiricum L-leucine + H2O + NAD+
-
r
L-isoleucine + H2O + NAD+
-
Exiguobacterium sibiricum 3-methyl-2-oxopentanoate + NH3 + NADH + H+
-
r
L-leucine + H2O + NAD+
-
Exiguobacterium sibiricum 4-methyl-2-oxopentanoate + NH3 + NADH + H+
-
r
L-valine + H2O + NAD+
-
Exiguobacterium sibiricum 3-methyl-2-oxobutanoate + NH3 + NADH + H+
-
r

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
half-life 11 h Exiguobacterium sibiricum
60
-
half-life 3.1 h Exiguobacterium sibiricum