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Literature summary for 1.4.1.9 extracted from

  • Liu, W.; Ma, H.; Luo, J.; Shen, W.; Xu, X.; Li, S.; Hu, Y.; Huang, H.
    Efficient synthesis of L-tert-leucine through reductive amination using leucine dehydrogenase and formate dehydrogenase coexpressed in recombinant E. coli (2014), Biochem. Eng. J., 91, 204-209 .
No PubMed abstract available
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Application

Application Comment Organism
synthesis coexpression with NAD+-dependent FDH from Candida boidinii in Escherichia coli for synthesis of L-tert-leucine. In a continuous feeding process, at an overall substrate concentration up to 1.5 M, both conversion and enantiomeric excess of >99% and space-time yield of 786 g/l/d are achieved Lysinibacillus sphaericus

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Lysinibacillus sphaericus

Organism

Organism UniProt Comment Textmining
Lysinibacillus sphaericus Q76GS2
-
-
Lysinibacillus sphaericus CGMCC 1.1677 Q76GS2
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-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3,3-dimethyl-2-oxobutanoate + NH3 + NADH
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 Lysinibacillus sphaericus L-tert-Leu + H2O + NAD+
-
 ?
3,3-dimethyl-2-oxobutanoate + NH3 + NADH
-
 Lysinibacillus sphaericus CGMCC 1.1677 L-tert-Leu + H2O + NAD+
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30 35
-
 Lysinibacillus sphaericus