General Stability | Organism |
---|---|
enzyme requires a high concentration of salt for stability | Natrialba magadii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
AgNO3 | 1 mM, complete | Natrialba magadii | |
HgCl2 | 1 mM, complete | Natrialba magadii | |
NADH | competitive inhibition versus NAD+, noncompetitive inhibition with respect to L-Leu | Natrialba magadii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
NADH | pH 9.5, 30°C | Natrialba magadii | |
0.48 | - |
NAD+ | pH 9.5, 30°C | Natrialba magadii | |
0.66 | - |
L-Ile | pH 9.5, 30°C, in presence of 4 M NaCL | Natrialba magadii | |
0.69 | - |
L-Leu | pH 9.5, 30°C | Natrialba magadii | |
0.71 | - |
L-Val | pH 9.5, 30°C, in presence of 4 M NaCL | Natrialba magadii | |
4 | - |
2-ketoisocaproate | pH 9.5, 30°C | Natrialba magadii | |
220 | - |
NH3 | pH 9.5, 30°C | Natrialba magadii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
CsCl | enzyme shows maximal activity in presence of more than 3 M salt | Natrialba magadii | |
KCl | enzyme shows maximal activity in presence of more than 3 M salt | Natrialba magadii | |
NaCl | enzyme shows maximal activity in presence of more than 3 M salt | Natrialba magadii | |
RbCl | enzyme shows maximal activity in presence of more than 4 M salt | Natrialba magadii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
55000 | - |
6 * 55000, at 2.5 M NaCl, dissociates into trimers and dimers in a lower concentration of salts, cetyltrimethylammonium-bromide-PAGE | Natrialba magadii |
330000 | - |
gel filtration | Natrialba magadii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Natrialba magadii | - |
- |
- |
Natrialba magadii MS-3 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Natrialba magadii |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.92 | - |
- |
Natrialba magadii |
Storage Stability | Organism |
---|---|
30°C, 2 months, 50% loss of activity in presence of 2.5 M NaCl | Natrialba magadii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-ketobutyrate + NH3 + NADH | 70% of the activity with 2-ketoisocaproate | Natrialba magadii | L-2-aminobutyrate + H2O + NAD+ | - |
? | |
2-ketobutyrate + NH3 + NADH | 70% of the activity with 2-ketoisocaproate | Natrialba magadii MS-3 | L-2-aminobutyrate + H2O + NAD+ | - |
? | |
2-ketocaproate + NH3 + NADH | 69% of the activity with 2-ketoisocaproate | Natrialba magadii | L-2-aminohexanoate + H2O + NAD+ | - |
? | |
2-ketoisocaproate + NH3 + NADH | - |
Natrialba magadii | L-Ile + H2O + NAD+ | - |
? | |
2-ketoisovalerate + NH3 + NADH | 150% of the activity with 2-ketoisocaproate | Natrialba magadii | L-Val + H2O + NAD+ | - |
? | |
2-ketovalerate + NH3 + NADH | 96% of the activity with 2-ketoisocaproate | Natrialba magadii | L-norvaline + H2O + NAD+ | - |
? | |
L-2-aminobutyrate + H2O + NAD+ | 32% of the activity with L-Leu | Natrialba magadii | 2-oxobutanoate + NH3 + NADH | - |
? | |
L-Ile + H2O + NAD+ | 72% of the activity with L-Leu | Natrialba magadii | 3-methyl-2-oxopentanoate + NH3 + NADH | - |
? | |
L-Ile + H2O + NAD+ | 72% of the activity with L-Leu | Natrialba magadii MS-3 | 3-methyl-2-oxopentanoate + NH3 + NADH | - |
? | |
L-Leu + H2O + NAD+ | - |
Natrialba magadii | 4-methyl-2-oxopentanoate + NH3 + NADH | - |
? | |
L-Leu + H2O + NAD+ | - |
Natrialba magadii MS-3 | 4-methyl-2-oxopentanoate + NH3 + NADH | - |
? | |
L-norvaline + H2O + NAD+ | 7% the activity with L-Leu | Natrialba magadii | 2-oxopentanoate + NH3 + NADH | - |
? | |
L-norvaline + H2O + NAD+ | 7% the activity with L-Leu | Natrialba magadii MS-3 | 2-oxopentanoate + NH3 + NADH | - |
? | |
L-Val + H2O + NAD+ | 59% of the activity with L-Leu | Natrialba magadii | 3-methyl-2-oxobutanoate + NH3 + NADH | - |
? | |
L-Val + H2O + NAD+ | 59% of the activity with L-Leu | Natrialba magadii MS-3 | 3-methyl-2-oxobutanoate + NH3 + NADH | - |
? | |
S-methyl-L-cysteine + H2O + NAD+ | 19% the activity with L-Leu | Natrialba magadii | 3-methylthio-2-oxopropanoate + NH3 + NADH | - |
? |
Subunits | Comment | Organism |
---|---|---|
hexamer | 6 * 55000, at 2.5 M NaCl, dissociates into trimers and dimers in a lower concentration of salts, cetyltrimethylammonium-bromide-PAGE | Natrialba magadii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
- |
Natrialba magadii |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
2 months, 50% loss of activity in presence of 2.5 M NaCl | Natrialba magadii |
50 | - |
1 h, stable in presence of 2.5 M NaCl or 2.5 M KCl | Natrialba magadii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9.5 | - |
oxidative deamination of L-Leu and reductive amination of 2-ketoisocaproate, in presence of 3 M NaCl | Natrialba magadii |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
8 | 10 | pH 8: about 30% of maximal activity, pH 10: about 70% of maximal activity, reductive amination of 2-ketoisocaproate | Natrialba magadii |
9 | 10.5 | pH 9: about 40% of maximal activity, pH 10.5: about 55% of maximal activity, oxidative deamination of L-Leu | Natrialba magadii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Natrialba magadii | |
NADH | - |
Natrialba magadii |