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Literature summary for 1.4.1.9 extracted from

  • Katoh, R.; Ngata, S.; Ozawa, A.; Ohshima, T.; Kamekura, M.; Misono, H.
    Purification and characterization of leucine dehydrogenase from an alkalophilic halophile, Natronobacterium magadii MS-3 (2003), J. Mol. Catal. B, 23, 231-238.
No PubMed abstract available

General Stability

General Stability Organism
enzyme requires a high concentration of salt for stability Natrialba magadii

Inhibitors

Inhibitors Comment Organism Structure
AgNO3 1 mM, complete Natrialba magadii
HgCl2 1 mM, complete Natrialba magadii
NADH competitive inhibition versus NAD+, noncompetitive inhibition with respect to L-Leu Natrialba magadii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.02
-
NADH pH 9.5, 30°C Natrialba magadii
0.48
-
NAD+ pH 9.5, 30°C Natrialba magadii
0.66
-
L-Ile pH 9.5, 30°C, in presence of 4 M NaCL Natrialba magadii
0.69
-
L-Leu pH 9.5, 30°C Natrialba magadii
0.71
-
L-Val pH 9.5, 30°C, in presence of 4 M NaCL Natrialba magadii
4
-
2-ketoisocaproate pH 9.5, 30°C Natrialba magadii
220
-
NH3 pH 9.5, 30°C Natrialba magadii

Metals/Ions

Metals/Ions Comment Organism Structure
CsCl enzyme shows maximal activity in presence of more than 3 M salt Natrialba magadii
KCl enzyme shows maximal activity in presence of more than 3 M salt Natrialba magadii
NaCl enzyme shows maximal activity in presence of more than 3 M salt Natrialba magadii
RbCl enzyme shows maximal activity in presence of more than 4 M salt Natrialba magadii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
55000
-
6 * 55000, at 2.5 M NaCl, dissociates into trimers and dimers in a lower concentration of salts, cetyltrimethylammonium-bromide-PAGE Natrialba magadii
330000
-
gel filtration Natrialba magadii

Organism

Organism UniProt Comment Textmining
Natrialba magadii
-
-
-
Natrialba magadii MS-3
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Natrialba magadii

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.92
-
-
Natrialba magadii

Storage Stability

Storage Stability Organism
30°C, 2 months, 50% loss of activity in presence of 2.5 M NaCl Natrialba magadii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-ketobutyrate + NH3 + NADH 70% of the activity with 2-ketoisocaproate Natrialba magadii L-2-aminobutyrate + H2O + NAD+
-
?
2-ketobutyrate + NH3 + NADH 70% of the activity with 2-ketoisocaproate Natrialba magadii MS-3 L-2-aminobutyrate + H2O + NAD+
-
?
2-ketocaproate + NH3 + NADH 69% of the activity with 2-ketoisocaproate Natrialba magadii L-2-aminohexanoate + H2O + NAD+
-
?
2-ketoisocaproate + NH3 + NADH
-
Natrialba magadii L-Ile + H2O + NAD+
-
?
2-ketoisovalerate + NH3 + NADH 150% of the activity with 2-ketoisocaproate Natrialba magadii L-Val + H2O + NAD+
-
?
2-ketovalerate + NH3 + NADH 96% of the activity with 2-ketoisocaproate Natrialba magadii L-norvaline + H2O + NAD+
-
?
L-2-aminobutyrate + H2O + NAD+ 32% of the activity with L-Leu Natrialba magadii 2-oxobutanoate + NH3 + NADH
-
?
L-Ile + H2O + NAD+ 72% of the activity with L-Leu Natrialba magadii 3-methyl-2-oxopentanoate + NH3 + NADH
-
?
L-Ile + H2O + NAD+ 72% of the activity with L-Leu Natrialba magadii MS-3 3-methyl-2-oxopentanoate + NH3 + NADH
-
?
L-Leu + H2O + NAD+
-
Natrialba magadii 4-methyl-2-oxopentanoate + NH3 + NADH
-
?
L-Leu + H2O + NAD+
-
Natrialba magadii MS-3 4-methyl-2-oxopentanoate + NH3 + NADH
-
?
L-norvaline + H2O + NAD+ 7% the activity with L-Leu Natrialba magadii 2-oxopentanoate + NH3 + NADH
-
?
L-norvaline + H2O + NAD+ 7% the activity with L-Leu Natrialba magadii MS-3 2-oxopentanoate + NH3 + NADH
-
?
L-Val + H2O + NAD+ 59% of the activity with L-Leu Natrialba magadii 3-methyl-2-oxobutanoate + NH3 + NADH
-
?
L-Val + H2O + NAD+ 59% of the activity with L-Leu Natrialba magadii MS-3 3-methyl-2-oxobutanoate + NH3 + NADH
-
?
S-methyl-L-cysteine + H2O + NAD+ 19% the activity with L-Leu Natrialba magadii 3-methylthio-2-oxopropanoate + NH3 + NADH
-
?

Subunits

Subunits Comment Organism
hexamer 6 * 55000, at 2.5 M NaCl, dissociates into trimers and dimers in a lower concentration of salts, cetyltrimethylammonium-bromide-PAGE Natrialba magadii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
65
-
-
Natrialba magadii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
2 months, 50% loss of activity in presence of 2.5 M NaCl Natrialba magadii
50
-
1 h, stable in presence of 2.5 M NaCl or 2.5 M KCl Natrialba magadii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.5
-
oxidative deamination of L-Leu and reductive amination of 2-ketoisocaproate, in presence of 3 M NaCl Natrialba magadii

pH Range

pH Minimum pH Maximum Comment Organism
8 10 pH 8: about 30% of maximal activity, pH 10: about 70% of maximal activity, reductive amination of 2-ketoisocaproate Natrialba magadii
9 10.5 pH 9: about 40% of maximal activity, pH 10.5: about 55% of maximal activity, oxidative deamination of L-Leu Natrialba magadii

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Natrialba magadii
NADH
-
Natrialba magadii