Protein Variants | Comment | Organism |
---|---|---|
K68A | nearly complete loss of activity in the oxidative deamination, marked increase in Km-values for both amino acid substrates and oxo acid substrates. An ionizable group in the wild-type enzyme with a pKa value of 10.1-10.7, which must be protonated for binding of substrate and competitive inhibitor with an alpha-carboxyl group, is unobservable in mutant enzyme | Geobacillus stearothermophilus |
K68R | nearly complete loss of activity in the oxidative deamination. An ionizable group in the wild-type enzyme with a pKa value of 10.1-10.7, which must be protonated for binding of substrate and competitive inhibitor with an alpha-carboxyl group, is unobservable in mutant enzyme | Geobacillus stearothermophilus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.021 | - |
NADH | mutant enzyme K68R | Geobacillus stearothermophilus | |
0.035 | - |
NADH | wild-type enzyme | Geobacillus stearothermophilus | |
0.053 | - |
NADH | mutant enzyme K68A | Geobacillus stearothermophilus | |
0.055 | - |
NAD+ | mutant enzyme K68R | Geobacillus stearothermophilus | |
0.063 | - |
NAD+ | wild-type enzyme | Geobacillus stearothermophilus | |
0.11 | - |
NAD+ | mutant enzyme K68A | Geobacillus stearothermophilus | |
0.88 | - |
2-Oxoisohexanoate | wild-type enzyme | Geobacillus stearothermophilus | |
1.2 | - |
4-methyl-2-oxopentanoate | mutant enzyme K68R | Geobacillus stearothermophilus | |
3.2 | - |
L-Leu | mutant enzyme K68R | Geobacillus stearothermophilus | |
5.1 | - |
L-Leu | wild-type enzyme | Geobacillus stearothermophilus | |
130 | - |
L-Leu | mutant enzyme K68A | Geobacillus stearothermophilus | |
140 | - |
4-methyl-2-oxopentanoate | mutant enzyme K68A | Geobacillus stearothermophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus stearothermophilus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
mutant enzymes K68A and K68R | Geobacillus stearothermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-Leu + H2O + NAD+ | - |
Geobacillus stearothermophilus | 4-methyl-2-oxopentanoate + NH3 + NADH | - |
r |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | turnover numbers of wild-type enzyme and mutant enzymes | Geobacillus stearothermophilus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Geobacillus stearothermophilus | |
NADH | - |
Geobacillus stearothermophilus |