Cloned (Comment) | Organism |
---|---|
- |
Thermoactinomyces intermedius |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.042 | - |
NADH | - |
Thermoactinomyces intermedius | |
0.36 | - |
NAD+ | - |
Thermoactinomyces intermedius | |
0.4 | - |
L-Ile | - |
Thermoactinomyces intermedius | |
0.63 | - |
4-methyl-2-oxopentanoate | - |
Thermoactinomyces intermedius | |
0.91 | - |
2-oxopentanoate | - |
Thermoactinomyces intermedius | |
1.4 | - |
2-Oxohexanoate | - |
Thermoactinomyces intermedius | |
2 | - |
L-Leu | - |
Thermoactinomyces intermedius | |
2.2 | - |
2-oxo-3-methylpentanoate | - |
Thermoactinomyces intermedius | |
2.4 | - |
L-Val | - |
Thermoactinomyces intermedius | |
2.7 | - |
4-methyl-2-oxo-5,5,5-trifluoropentanoate | - |
Thermoactinomyces intermedius | |
2.8 | - |
NADP+ | mutant enzyme D203A/I204R | Thermoactinomyces intermedius | |
4.4 | - |
3-methyl-2-oxobutanoate | - |
Thermoactinomyces intermedius | |
7.2 | - |
2-oxobutanoate | - |
Thermoactinomyces intermedius |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
42000 | - |
4 * 42000, SDS-PAGE | Thermoactinomyces intermedius |
340000 | - |
gel filtration | Thermoactinomyces intermedius |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermoactinomyces intermedius | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Thermoactinomyces intermedius |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
102 | - |
- |
Thermoactinomyces intermedius |
122 | - |
recombinant enzyme | Thermoactinomyces intermedius |
Storage Stability | Organism |
---|---|
4°C, stable for over two years | Thermoactinomyces intermedius |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxohexanoate + NH3 + NADH + H+ | 46% activity compared to 2-oxoisocaproate | Thermoactinomyces intermedius | L-norleucine + H2O + NAD+ | - |
r | |
4-methyl-2-oxo-5,5,5-trifluoropentanoate + NH3 + NADH | - |
Thermoactinomyces intermedius | 2-amino-4-methyl-5,5,5-trifluoropentanoate + NAD+ + H2O | - |
? | |
L-2-aminobutanoate + H2O + NAD+ | - |
Thermoactinomyces intermedius | 2-oxobutanoate + NH3 + NADH + H+ | - |
? | |
L-2-aminobutanoate + H2O + NAD+ | - |
Thermoactinomyces intermedius | 2-oxobutanoate + NH3 + NADH + H+ | - |
r | |
L-Ile + H2O + NAD+ | - |
Thermoactinomyces intermedius | 3-methyl-2-oxopentanoate + NH3 + NADH | - |
r | |
L-Leu + H2O + NAD+ | - |
Thermoactinomyces intermedius | 4-methyl-2-oxopentanoate + NH3 + NADH | - |
r | |
L-Val + H2O + NAD+ | - |
Thermoactinomyces intermedius | 3-methyl-2-oxobutanoate + NH3 + NADH | - |
r |
Subunits | Comment | Organism |
---|---|---|
octamer | 4 * 42000, SDS-PAGE | Thermoactinomyces intermedius |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
10 min, retains its full activity | Thermoactinomyces intermedius |
70 | - |
40 min, 3 M NaCl, less than 10% loss of activity | Thermoactinomyces intermedius |
75 | - |
10 min, about 75% loss of activity | Thermoactinomyces intermedius |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
reductive amination of 3-methyl-2-oxobutanoate | Thermoactinomyces intermedius |
9.3 | - |
reductive amination of 2-oxo-3-methylpentanoate | Thermoactinomyces intermedius |
9.6 | - |
reductive amination of 4-methyl-2-oxopentanoate | Thermoactinomyces intermedius |
10 | - |
deamination of L-Val | Thermoactinomyces intermedius |
10.5 | - |
deamination of L-Leu | Thermoactinomyces intermedius |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 10.9 | 50°C, 5 min, stable | Thermoactinomyces intermedius |
7.5 | - |
70°C, 5 min, most stable at pH 7.5 | Thermoactinomyces intermedius |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Thermoactinomyces intermedius | |
NADH | - |
Thermoactinomyces intermedius |