Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli C600 | Moorella thermoacetica |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.8 | - |
4-methyl-2-oxopentanoate | - |
Moorella thermoacetica | |
1 | - |
2-oxopentanoate | - |
Moorella thermoacetica | |
1.5 | - |
L-2-aminobutanoate | - |
Moorella thermoacetica | |
2.3 | - |
L-norleucine | - |
Moorella thermoacetica | |
3.9 | - |
L-Ile | - |
Moorella thermoacetica | |
4 | - |
3-methyl-2-oxobutanoate | - |
Moorella thermoacetica | |
5.6 | - |
L-norvaline | - |
Moorella thermoacetica | |
6.2 | - |
2-Oxohexanoate | - |
Moorella thermoacetica | |
6.8 | - |
L-Val | - |
Moorella thermoacetica | |
7.3 | - |
2-oxobutanoate | - |
Moorella thermoacetica | |
8.5 | - |
L-Leu | - |
Moorella thermoacetica |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
56000 | - |
6 * 56000, SDS-PAGE | Moorella thermoacetica |
350000 | - |
gel filtration | Moorella thermoacetica |
360000 | - |
equilibrium sedimentation | Moorella thermoacetica |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Moorella thermoacetica | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Moorella thermoacetica |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
115 | - |
- |
Moorella thermoacetica |
Storage Stability | Organism |
---|---|
4°C, buffer containing 0.02% azide, stable for more than 1 year | Moorella thermoacetica |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-2-aminobutanoate + H2O + NAD+ | 11% of the activity with L-Leu | Moorella thermoacetica | 2-oxobutanoate + NH3 + NADH + H+ | - |
r | |
L-2-aminobutanoate + H2O + NAD+ | 19% of the activity with L-Leu | Moorella thermoacetica | 2-oxobutanoate + NH3 + NADH + H+ | - |
r | |
L-Ile + H2O + NAD+ | 88% of the activity with L-Leu | Moorella thermoacetica | 3-methyl-2-oxopentanoate + NH3 + NADH | - |
r | |
L-Leu + H2O + NAD+ | - |
Moorella thermoacetica | 4-methyl-2-oxopentanoate + NH3 + NADH | - |
r | |
L-norleucine + H2O + NAD+ | 15% of the activity with L-Leu | Moorella thermoacetica | 2-oxohexanoate + NH3 + NADH | - |
r | |
L-norvaline + H2O + NAD+ | - |
Moorella thermoacetica | 2-oxopentanoate + NH3 + NADH + H+ | - |
r | |
L-Val + H2O + NAD+ | 93% of the activity with L-Leu | Moorella thermoacetica | 3-methyl-2-oxobutanoate + NH3 + NADH | - |
r | |
additional information | the enzyme is B-stereospecific | Moorella thermoacetica | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
hexamer | 6 * 56000, SDS-PAGE | Moorella thermoacetica |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
pH 6.0-10.0, 10 min, stable | Moorella thermoacetica |
75 | - |
30 min, stable | Moorella thermoacetica |
85 | - |
5 min, substantial loss of activity | Moorella thermoacetica |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9.5 | - |
reductive amination of 3-methyl-2-oxobutanoate | Moorella thermoacetica |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 10 | 55°C, 10 min, stable | Moorella thermoacetica |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | no activity with NADPH | Moorella thermoacetica | |
additional information | no activity with NADP+ | Moorella thermoacetica | |
NAD+ | - |
Moorella thermoacetica | |
NADH | - |
Moorella thermoacetica |