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Literature summary for 1.4.1.9 extracted from

  • Shimoi, H.; Nagata, S.; Esaki, N.; Tanaka, H.; Soda, K.
    Leucine dehydrogenase of a thermophilic anaerobe, Clostridium thermoaceticum: gene cloning, purification and characterization (1987), Agric. Biol. Chem., 51, 3375-3381.
No PubMed abstract available

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli C600 Moorella thermoacetica

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.8
-
4-methyl-2-oxopentanoate
-
Moorella thermoacetica
1
-
2-oxopentanoate
-
Moorella thermoacetica
1.5
-
L-2-aminobutanoate
-
Moorella thermoacetica
2.3
-
L-norleucine
-
Moorella thermoacetica
3.9
-
L-Ile
-
Moorella thermoacetica
4
-
3-methyl-2-oxobutanoate
-
Moorella thermoacetica
5.6
-
L-norvaline
-
Moorella thermoacetica
6.2
-
2-Oxohexanoate
-
Moorella thermoacetica
6.8
-
L-Val
-
Moorella thermoacetica
7.3
-
2-oxobutanoate
-
Moorella thermoacetica
8.5
-
L-Leu
-
Moorella thermoacetica

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
56000
-
6 * 56000, SDS-PAGE Moorella thermoacetica
350000
-
gel filtration Moorella thermoacetica
360000
-
equilibrium sedimentation Moorella thermoacetica

Organism

Organism UniProt Comment Textmining
Moorella thermoacetica
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Moorella thermoacetica

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
115
-
-
Moorella thermoacetica

Storage Stability

Storage Stability Organism
4°C, buffer containing 0.02% azide, stable for more than 1 year Moorella thermoacetica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-2-aminobutanoate + H2O + NAD+ 11% of the activity with L-Leu Moorella thermoacetica 2-oxobutanoate + NH3 + NADH + H+
-
r
L-2-aminobutanoate + H2O + NAD+ 19% of the activity with L-Leu Moorella thermoacetica 2-oxobutanoate + NH3 + NADH + H+
-
r
L-Ile + H2O + NAD+ 88% of the activity with L-Leu Moorella thermoacetica 3-methyl-2-oxopentanoate + NH3 + NADH
-
r
L-Leu + H2O + NAD+
-
Moorella thermoacetica 4-methyl-2-oxopentanoate + NH3 + NADH
-
r
L-norleucine + H2O + NAD+ 15% of the activity with L-Leu Moorella thermoacetica 2-oxohexanoate + NH3 + NADH
-
r
L-norvaline + H2O + NAD+
-
Moorella thermoacetica 2-oxopentanoate + NH3 + NADH + H+
-
r
L-Val + H2O + NAD+ 93% of the activity with L-Leu Moorella thermoacetica 3-methyl-2-oxobutanoate + NH3 + NADH
-
r
additional information the enzyme is B-stereospecific Moorella thermoacetica ?
-
?

Subunits

Subunits Comment Organism
hexamer 6 * 56000, SDS-PAGE Moorella thermoacetica

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
pH 6.0-10.0, 10 min, stable Moorella thermoacetica
75
-
30 min, stable Moorella thermoacetica
85
-
5 min, substantial loss of activity Moorella thermoacetica

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.5
-
reductive amination of 3-methyl-2-oxobutanoate Moorella thermoacetica

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 10 55°C, 10 min, stable Moorella thermoacetica

Cofactor

Cofactor Comment Organism Structure
additional information no activity with NADPH Moorella thermoacetica
additional information no activity with NADP+ Moorella thermoacetica
NAD+
-
Moorella thermoacetica
NADH
-
Moorella thermoacetica