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Literature summary for 1.4.1.9 extracted from
- Flow-injection determination of branched-chain L-amino acids with immobilized leucine dehydrogenase (1989), Anal. Chim. Acta, 218, 161-166.
No PubMed abstract available
Application
| Application | Comment | Organism |
|---|---|---|
| analysis | flow-injection determination of branched-chain L-amino acids with immobilized leucine dehydrogenase | Bacillus sp. (in: Bacteria) |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | - |
Bacillus sp. (in: Bacteria) |  |
| Cu2+ | - |
Bacillus sp. (in: Bacteria) | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus cereus | - |
- |
- |
| Bacillus sp. (in: Bacteria) | - |
- |
- |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, 5 mM tetrasodium EDTA solution in phosphate buffer, 0.1 M, pH 7.0, stable for at least 2 months | Bacillus sp. (in: Bacteria) |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-Leu + H2O + NAD+ | - |
Bacillus cereus | 4-methyl-2-oxopentanoate + NH3 + NADH | - |
r | |
| L-Leu + H2O + NAD+ | - |
Bacillus sp. (in: Bacteria) | 4-methyl-2-oxopentanoate + NH3 + NADH | - |
r | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Bacillus sp. (in: Bacteria) |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 52 | 30°C: about 45% of maximal activity, 48-52°C: maximal activity | Bacillus sp. (in: Bacteria) |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 52 | - |
denaturation above | Bacillus sp. (in: Bacteria) |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 11 | - |
oxidative deamination of L-Leu | Bacillus sp. (in: Bacteria) |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 10 | 11.5 | pH 10.0: about 40% of maximal activity, pH 11.5: about 80% of maximal activity, oxidative deamination of L-Leu | Bacillus sp. (in: Bacteria) |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Bacillus cereus | |
| NAD+ | - |
Bacillus sp. (in: Bacteria) | |
| NADH | - |
Bacillus cereus | |
| NADH | - |
Bacillus sp. (in: Bacteria) | |
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Release 2023.1 (January 2023)
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