Properties of crystalline leucine dehydrogenase from Bacillus sphaericus
Ohshima, T.; Misono, H.; Soda, K.; J. Biol. Chem. 253, 5719-5725 (1978) 
Data extracted from this reference:
Crystallization (Commentary)
Crystallization (Commentary)
Organism
crystallized by addition of ammonium sulfate
Lysinibacillus sphaericus
Inhibitors
Inhibitors
Commentary
Organism
Structure
Co2+
CoCl2
Lysinibacillus sphaericus
Cu2+
CuSO4
Lysinibacillus sphaericus
D-2-aminobutanoate
-
Lysinibacillus sphaericus
D-alloisoleucine
-
Lysinibacillus sphaericus
D-Leu
-
Lysinibacillus sphaericus
D-norleucine
-
Lysinibacillus sphaericus
D-Norvaline
-
Lysinibacillus sphaericus
D-Val
-
Lysinibacillus sphaericus
HgCl2
-
Bacillus cereus
HgCl2
-
Lysinibacillus sphaericus
MgCl2
-
Lysinibacillus sphaericus
Na2S
-
Lysinibacillus sphaericus
NAD+
product inhibition
Lysinibacillus sphaericus
PCMB
-
Bacillus cereus
PCMB
-
Lysinibacillus sphaericus
pyridoxal 5'-phosphate
-
Lysinibacillus sphaericus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.31
-
4-methyl-2-oxopentanoate
-
Lysinibacillus sphaericus
0.39
-
NAD+
-
Lysinibacillus sphaericus
0.77
-
3-acetylpyridine-NAD+
-
Lysinibacillus sphaericus
1
-
L-Leu
-
Lysinibacillus sphaericus
1.4
-
3-methyl-2-oxobutanoate
-
Lysinibacillus sphaericus
1.7
-
L-Val
-
Lysinibacillus sphaericus
1.7
-
2-Oxopentanoate
-
Lysinibacillus sphaericus
1.8
-
L-Ile
-
Lysinibacillus sphaericus
2.41
-
3-acetylpyridine-deamino-NAD+
-
Lysinibacillus sphaericus
3.5
-
L-norvaline
-
Lysinibacillus sphaericus
6.3
-
L-norleucine
-
Lysinibacillus sphaericus
7
-
2-Oxohexanoate
-
Lysinibacillus sphaericus
7.7
-
2-oxobutanoate
-
Lysinibacillus sphaericus
10
-
L-2-aminobutanoate
-
Lysinibacillus sphaericus
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
41000
-
6 * 41000, SDS-PAGE
Lysinibacillus sphaericus
245000
-
equilibrium sedimentation
Lysinibacillus sphaericus
Organism
Organism
UniProt
Commentary
Textmining
Bacillus cereus
-
-
-
Bacillus pumilus
-
var. flavus
-
Bacillus subtilis
-
-
-
Brevibacillus brevis
-
-
-
Lysinibacillus sphaericus
-
-
-
Priestia megaterium
-
-
-
Purification (Commentary)
Purification (Commentary)
Organism
-
Lysinibacillus sphaericus
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
42.3
-
-
Lysinibacillus sphaericus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
L-2-aminobutanoate + H2O + NAD+
-
349665
Bacillus cereus
2-oxobutanoate + NH3 + NADH
-
349665
Bacillus cereus
r
L-2-aminobutanoate + H2O + NAD+
14% of the activity with L-Leu
349665
Lysinibacillus sphaericus
2-oxobutanoate + NH3 + NADH
-
349665
Lysinibacillus sphaericus
r
L-2-aminobutanoate + H2O + NAD+
-
349665
Bacillus subtilis
2-oxopentanoate + NH3 + NADH
-
-
-
r
L-2-aminobutanoate + H2O + NAD+
-
349665
Bacillus cereus
2-oxopentanoate + NH3 + NADH
-
-
-
r
L-2-aminobutanoate + H2O + NAD+
41% of the activity with L-Leu
349665
Lysinibacillus sphaericus
2-oxopentanoate + NH3 + NADH
-
-
-
r
L-Ile + H2O + NAD+
-
349665
Bacillus subtilis
3-methyl-2-oxopentanoate + NH3 + NADH
-
-
-
r
L-Ile + H2O + NAD+
-
349665
Bacillus cereus
3-methyl-2-oxopentanoate + NH3 + NADH
-
-
-
r
L-Ile + H2O + NAD+
58% of the activity with L-Leu
349665
Lysinibacillus sphaericus
3-methyl-2-oxopentanoate + NH3 + NADH
-
-
-
r
L-Leu + H2O + 3-acetylpyridine-deamino-NAD+
as effective as NAD+
349665
Lysinibacillus sphaericus
4-methyl-2-oxopentanoate + NH3 + 3-acetylpyridine-deamino-NADH
-
-
-
?
L-Leu + H2O + 3-acetylpyridine-NAD+
166% of the activity with NAD+
349665
Lysinibacillus sphaericus
4-methyl-2-oxopentanoate + NH3 + 3-acetylpyridine-NADH
-
-
-
?
L-Leu + H2O + 3-pyridinealdehyde-NAD+
19% of the activity with NAD+
349665
Lysinibacillus sphaericus
4-methyl-2-oxopentanoate + NH3 + 3-pyridinealdehyde-NADH
-
-
-
?
L-Leu + H2O + deamino-NAD+
81% of the activity with NAD+
349665
Lysinibacillus sphaericus
4-methyl-2-oxopentanoate + NH3 + deamino-NADH
-
-
-
?
L-Leu + H2O + NAD+
-
349665
Brevibacillus brevis
4-methyl-2-oxopentanoate + NH3 + NADH
-
349665
Brevibacillus brevis
r
L-Leu + H2O + NAD+
-
349665
Bacillus subtilis
4-methyl-2-oxopentanoate + NH3 + NADH
-
349665
Bacillus subtilis
r
L-Leu + H2O + NAD+
-
349665
Priestia megaterium
4-methyl-2-oxopentanoate + NH3 + NADH
-
349665
Priestia megaterium
r
L-Leu + H2O + NAD+
-
349665
Lysinibacillus sphaericus
4-methyl-2-oxopentanoate + NH3 + NADH
-
349665
Lysinibacillus sphaericus
r
L-Leu + H2O + NAD+
-
349665
Bacillus cereus
4-methyl-2-oxopentanoate + NH3 + NADH
-
349665
Bacillus cereus
r
L-Leu + H2O + NAD+
-
349665
Bacillus pumilus
4-methyl-2-oxopentanoate + NH3 + NADH
-
349665
Bacillus pumilus
r
L-Leu + H2O + thionicotinamide-NAD+
21% of the activity with NAD+
349665
Lysinibacillus sphaericus
4-methyl-2-oxopentanoate + NH3 + thionicotinamide-NADH
-
-
-
?
L-norleucine + H2O + NAD+
-
349665
Bacillus subtilis
2-oxohexanoate + NH3 + NADH
-
-
-
?
L-norleucine + H2O + NAD+
-
349665
Bacillus cereus
2-oxohexanoate + NH3 + NADH
-
349665
Bacillus cereus
r
L-norleucine + H2O + NAD+
74% of the activity with L-Leu
349665
Lysinibacillus sphaericus
2-oxohexanoate + NH3 + NADH
-
349665
Lysinibacillus sphaericus
r
L-tert-Leu + H2O + NAD+
-
349665
Lysinibacillus sphaericus
3,3-dimethyl-2-oxobutanoate + NH3 + NADH
-
-
-
?
L-Val + H2O + NAD+
-
349665
Bacillus subtilis
3-methyl-2-oxobutanoate + NH3 + NADH
-
-
-
?
L-Val + H2O + NAD+
-
349665
Bacillus cereus
3-methyl-2-oxobutanoate + NH3 + NADH
-
-
-
?
L-Val + H2O + NAD+
74% of the activity with L-Leu
349665
Lysinibacillus sphaericus
3-methyl-2-oxobutanoate + NH3 + NADH
-
-
-
?
additional information
the enzyme is B-stereospecific
349665
Lysinibacillus sphaericus
?
-
-
-
?
additional information
reductive amination proceeds through a sequential ordered ternary-binary mechanism
349665
Lysinibacillus sphaericus
?
-
-
-
?
Subunits
Subunits
Commentary
Organism
hexamer
6 * 41000, SDS-PAGE
Lysinibacillus sphaericus
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
-
Brevibacillus brevis
NAD+
-
Bacillus subtilis
NAD+
-
Priestia megaterium
NAD+
-
Lysinibacillus sphaericus
NAD+
-
Bacillus cereus
NAD+
-
Bacillus pumilus
NADH
-
Brevibacillus brevis
NADH
-
Bacillus subtilis
NADH
-
Priestia megaterium
NADH
-
Lysinibacillus sphaericus
NADH
-
Bacillus cereus
NADH
-
Bacillus pumilus
Ki Value [mM]
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
2.2
-
D-Leu
inhibition of deamination of D-Leu
Lysinibacillus sphaericus
3
-
D-Norvaline
inhibition of deamination of D-Leu
Lysinibacillus sphaericus
7.9
-
D-alloisoleucine
inhibition of deamination of D-Leu
Lysinibacillus sphaericus
9.4
-
D-2-aminobutanoate
inhibition of deamination of D-Leu
Lysinibacillus sphaericus
10.8
-
D-Val
inhibition of deamination of D-Leu
Lysinibacillus sphaericus
52.6
-
D-norleucine
inhibition of deamination of D-Leu
Lysinibacillus sphaericus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
-
Brevibacillus brevis
NAD+
-
Bacillus subtilis
NAD+
-
Priestia megaterium
NAD+
-
Lysinibacillus sphaericus
NAD+
-
Bacillus cereus
NAD+
-
Bacillus pumilus
NADH
-
Brevibacillus brevis
NADH
-
Bacillus subtilis
NADH
-
Priestia megaterium
NADH
-
Lysinibacillus sphaericus
NADH
-
Bacillus cereus
NADH
-
Bacillus pumilus
Crystallization (Commentary) (protein specific)
Crystallization
Organism
crystallized by addition of ammonium sulfate
Lysinibacillus sphaericus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Co2+
CoCl2
Lysinibacillus sphaericus
Cu2+
CuSO4
Lysinibacillus sphaericus
D-2-aminobutanoate
-
Lysinibacillus sphaericus
D-alloisoleucine
-
Lysinibacillus sphaericus
D-Leu
-
Lysinibacillus sphaericus
D-norleucine
-
Lysinibacillus sphaericus
D-Norvaline
-
Lysinibacillus sphaericus
D-Val
-
Lysinibacillus sphaericus
HgCl2
-
Lysinibacillus sphaericus
HgCl2
-
Bacillus cereus
MgCl2
-
Lysinibacillus sphaericus
Na2S
-
Lysinibacillus sphaericus
NAD+
product inhibition
Lysinibacillus sphaericus
PCMB
-
Lysinibacillus sphaericus
PCMB
-
Bacillus cereus
pyridoxal 5'-phosphate
-
Lysinibacillus sphaericus
Ki Value [mM] (protein specific)
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
2.2
-
D-Leu
inhibition of deamination of D-Leu
Lysinibacillus sphaericus
3
-
D-Norvaline
inhibition of deamination of D-Leu
Lysinibacillus sphaericus
7.9
-
D-alloisoleucine
inhibition of deamination of D-Leu
Lysinibacillus sphaericus
9.4
-
D-2-aminobutanoate
inhibition of deamination of D-Leu
Lysinibacillus sphaericus
10.8
-
D-Val
inhibition of deamination of D-Leu
Lysinibacillus sphaericus
52.6
-
D-norleucine
inhibition of deamination of D-Leu
Lysinibacillus sphaericus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.31
-
4-methyl-2-oxopentanoate
-
Lysinibacillus sphaericus
0.39
-
NAD+
-
Lysinibacillus sphaericus
0.77
-
3-acetylpyridine-NAD+
-
Lysinibacillus sphaericus
1
-
L-Leu
-
Lysinibacillus sphaericus
1.4
-
3-methyl-2-oxobutanoate
-
Lysinibacillus sphaericus
1.7
-
L-Val
-
Lysinibacillus sphaericus
1.7
-
2-Oxopentanoate
-
Lysinibacillus sphaericus
1.8
-
L-Ile
-
Lysinibacillus sphaericus
2.41
-
3-acetylpyridine-deamino-NAD+
-
Lysinibacillus sphaericus
3.5
-
L-norvaline
-
Lysinibacillus sphaericus
6.3
-
L-norleucine
-
Lysinibacillus sphaericus
7
-
2-Oxohexanoate
-
Lysinibacillus sphaericus
7.7
-
2-oxobutanoate
-
Lysinibacillus sphaericus
10
-
L-2-aminobutanoate
-
Lysinibacillus sphaericus
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
41000
-
6 * 41000, SDS-PAGE
Lysinibacillus sphaericus
245000
-
equilibrium sedimentation
Lysinibacillus sphaericus
Purification (Commentary) (protein specific)
Commentary
Organism
-
Lysinibacillus sphaericus
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
42.3
-
-
Lysinibacillus sphaericus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
L-2-aminobutanoate + H2O + NAD+
-
349665
Bacillus cereus
2-oxobutanoate + NH3 + NADH
-
349665
Bacillus cereus
r
L-2-aminobutanoate + H2O + NAD+
14% of the activity with L-Leu
349665
Lysinibacillus sphaericus
2-oxobutanoate + NH3 + NADH
-
349665
Lysinibacillus sphaericus
r
L-2-aminobutanoate + H2O + NAD+
-
349665
Bacillus subtilis
2-oxopentanoate + NH3 + NADH
-
-
-
r
L-2-aminobutanoate + H2O + NAD+
-
349665
Bacillus cereus
2-oxopentanoate + NH3 + NADH
-
-
-
r
L-2-aminobutanoate + H2O + NAD+
41% of the activity with L-Leu
349665
Lysinibacillus sphaericus
2-oxopentanoate + NH3 + NADH
-
-
-
r
L-Ile + H2O + NAD+
-
349665
Bacillus subtilis
3-methyl-2-oxopentanoate + NH3 + NADH
-
-
-
r
L-Ile + H2O + NAD+
-
349665
Bacillus cereus
3-methyl-2-oxopentanoate + NH3 + NADH
-
-
-
r
L-Ile + H2O + NAD+
58% of the activity with L-Leu
349665
Lysinibacillus sphaericus
3-methyl-2-oxopentanoate + NH3 + NADH
-
-
-
r
L-Leu + H2O + 3-acetylpyridine-deamino-NAD+
as effective as NAD+
349665
Lysinibacillus sphaericus
4-methyl-2-oxopentanoate + NH3 + 3-acetylpyridine-deamino-NADH
-
-
-
?
L-Leu + H2O + 3-acetylpyridine-NAD+
166% of the activity with NAD+
349665
Lysinibacillus sphaericus
4-methyl-2-oxopentanoate + NH3 + 3-acetylpyridine-NADH
-
-
-
?
L-Leu + H2O + 3-pyridinealdehyde-NAD+
19% of the activity with NAD+
349665
Lysinibacillus sphaericus
4-methyl-2-oxopentanoate + NH3 + 3-pyridinealdehyde-NADH
-
-
-
?
L-Leu + H2O + deamino-NAD+
81% of the activity with NAD+
349665
Lysinibacillus sphaericus
4-methyl-2-oxopentanoate + NH3 + deamino-NADH
-
-
-
?
L-Leu + H2O + NAD+
-
349665
Brevibacillus brevis
4-methyl-2-oxopentanoate + NH3 + NADH
-
349665
Brevibacillus brevis
r
L-Leu + H2O + NAD+
-
349665
Bacillus subtilis
4-methyl-2-oxopentanoate + NH3 + NADH
-
349665
Bacillus subtilis
r
L-Leu + H2O + NAD+
-
349665
Priestia megaterium
4-methyl-2-oxopentanoate + NH3 + NADH
-
349665
Priestia megaterium
r
L-Leu + H2O + NAD+
-
349665
Lysinibacillus sphaericus
4-methyl-2-oxopentanoate + NH3 + NADH
-
349665
Lysinibacillus sphaericus
r
L-Leu + H2O + NAD+
-
349665
Bacillus cereus
4-methyl-2-oxopentanoate + NH3 + NADH
-
349665
Bacillus cereus
r
L-Leu + H2O + NAD+
-
349665
Bacillus pumilus
4-methyl-2-oxopentanoate + NH3 + NADH
-
349665
Bacillus pumilus
r
L-Leu + H2O + thionicotinamide-NAD+
21% of the activity with NAD+
349665
Lysinibacillus sphaericus
4-methyl-2-oxopentanoate + NH3 + thionicotinamide-NADH
-
-
-
?
L-norleucine + H2O + NAD+
-
349665
Bacillus subtilis
2-oxohexanoate + NH3 + NADH
-
-
-
?
L-norleucine + H2O + NAD+
-
349665
Bacillus cereus
2-oxohexanoate + NH3 + NADH
-
349665
Bacillus cereus
r
L-norleucine + H2O + NAD+
74% of the activity with L-Leu
349665
Lysinibacillus sphaericus
2-oxohexanoate + NH3 + NADH
-
349665
Lysinibacillus sphaericus
r
L-tert-Leu + H2O + NAD+
-
349665
Lysinibacillus sphaericus
3,3-dimethyl-2-oxobutanoate + NH3 + NADH
-
-
-
?
L-Val + H2O + NAD+
-
349665
Bacillus subtilis
3-methyl-2-oxobutanoate + NH3 + NADH
-
-
-
?
L-Val + H2O + NAD+
-
349665
Bacillus cereus
3-methyl-2-oxobutanoate + NH3 + NADH
-
-
-
?
L-Val + H2O + NAD+
74% of the activity with L-Leu
349665
Lysinibacillus sphaericus
3-methyl-2-oxobutanoate + NH3 + NADH
-
-
-
?
additional information
the enzyme is B-stereospecific
349665
Lysinibacillus sphaericus
?
-
-
-
?
additional information
reductive amination proceeds through a sequential ordered ternary-binary mechanism
349665
Lysinibacillus sphaericus
?
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
hexamer
6 * 41000, SDS-PAGE
Lysinibacillus sphaericus
Other publictions for EC 1.4.1.9
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
742376
Zhang
-
Engineering bi-functional enz ...
Bacillus cereus, Bacillus cereus ATCC 10987
Eng. Life Sci.
17
989-996
2017
-
1
1
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8
-
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2
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1
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2
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1
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1
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1
1
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8
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1
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2
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1
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1
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741652
Lu
The development of leucine de ...
Bacillus cereus
Appl. Biochem. Biotechnol.
180
1180-1195
2016
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1
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-
-
-
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4
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1
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Cloning, protein sequence clar ...
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7
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Katoh
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Cloning and sequencing of the ...
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2
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1
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6
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Kataoka
-
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2003
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349692
Muranova
Crystallization and preliminar ...
Lysinibacillus sphaericus, Thermoactinomyces intermedius
Acta Crystallogr. Sect. D
58
1059-1062
2002
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Oikawa
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280
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2001
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Ansorge
Production of recombinant L-le ...
Bacillus cereus
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557-562
2000
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349686
Galkin
Construction of a new leucine ...
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3
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Morishita
Kinetic assay of serum and uri ...
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349689
Kiba
-
Postcolumn co-immobilized leuc ...
Geobacillus stearothermophilus
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724
354-357
1996
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Nagata
Gene cloning, purification, an ...
Bacillus licheniformis
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349688
Baker
A role for quaternary structur ...
Lysinibacillus sphaericus
Structure
3
693-705
1995
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The purification, characteriza ...
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Sekimoto
Role of the conserved glycyl r ...
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Sekimoto
Involvement of conserved lysin ...
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Turnbull
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Sekimoto
Evidence for lysine 80 as gene ...
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Enzyme-catalyzed asymetric syn ...
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1991
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1
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Gene cloning, purification, an ...
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349695
Gu
Production of essential L-bran ...
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Biotechnol. Bioeng.
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1990
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1
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349696
Gu
Conversion of ammonia or urea ...
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Biotechnol. Appl. Biochem.
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1990
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1
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349664
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Purification and characterizat ...
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3
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18
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3
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1
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1
1
11
1
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1
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349670
Schustolla
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Gene cloning and sequence dete ...
Bacillus cereus
DECHEMA Biotechnol. Conf.
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1097-1101
1989
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1
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349674
Oka
Overproduction of thermostable ...
Geobacillus stearothermophilus
Biotechnol. Appl. Biochem.
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307-311
1989
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1
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1
2
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1
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1
1
1
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2
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1
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349676
Esaki
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Enantioselective synthesis of ...
Geobacillus stearothermophilus
Biotechnol. Appl. Biochem.
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312-317
1989
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1
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2
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1
1
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349678
Kiba
-
Flow-injection determination o ...
Bacillus cereus, Bacillus sp. (in: Bacteria)
Anal. Chim. Acta
218
161-166
1989
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1
1
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4
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1
2
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1
1
1
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1
1
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349699
Kiba
High-performance liquid chroma ...
Bacillus subtilis
J. Chromatogr.
497
236-242
1989
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1
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2
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1
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349663
Livesey
Isolation and characterization ...
Bacillus subtilis
Methods Enzymol.
166
282-288
1988
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3
9
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1
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1
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7
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1
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2
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1
9
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1
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7
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2
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349669
Nagata
Gene cloning and sequence dete ...
Geobacillus stearothermophilus
Biochemistry
27
9056-9062
1988
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1
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9
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1
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1
2
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1
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1
1
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349697
Livesey
Determination of branched-chai ...
Bacillus subtilis, Lysinibacillus sphaericus
Methods Enzymol.
166
3-10
1988
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2
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4
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6
-
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349680
Shimoi
-
Leucine dehydrogenase of a the ...
Moorella thermoacetica
Agric. Biol. Chem.
51
3375-3381
1987
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1
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1
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1
4
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11
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3
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1
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1
1
8
1
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3
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1
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1
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349554
Ohshima
-
Screening of thermostable leuc ...
Bacillus sp. (in: Bacteria), Lysinibacillus sphaericus
Biotechnol. Lett.
7
871-876
1985
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4
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349661
Schuette
-
L-Leucine dehydrogenase from B ...
Bacillus cereus
Appl. Microbiol. Biotechnol.
22
306-317
1985
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1
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3
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16
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2
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1
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1
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8
1
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2
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1
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349662
Ohshima
-
Purification and characterizat ...
Geobacillus stearothermophilus
Arch. Microbiol.
141
407-411
1985
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5
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1
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1
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1
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1
1
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3
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1
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4
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5
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2
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1
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1
1
4
1
1
1
2
-
3
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1
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349673
Ohshima
-
Modification of leucine dehydr ...
Lysinibacillus sphaericus
Agric. Biol. Chem.
48
349-354
1984
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1
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-
2
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-
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-
-
2
-
-
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-
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349668
Hummel
-
Leucine dehydrogenase from Bac ...
Lysinibacillus sphaericus
Eur. J. Appl. Microbiol. Biotechnol.
12
22-27
1981
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-
-
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1
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1
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2
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-
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-
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2
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1
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-
1
-
1
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349679
Warth
Heat stability of Bacillus cer ...
Bacillus cereus
J. Bacteriol.
143
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1980
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2
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1
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2
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349671
Obermeier
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Experiments on the role of leu ...
Bacillus subtilis, Bacillus subtilis 168M
FEMS Microbiol. Lett.
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81-83
1979
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4
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349665
Ohshima
Properties of crystalline leuc ...
Brevibacillus brevis, Bacillus cereus, Priestia megaterium, Bacillus subtilis, Bacillus pumilus, Lysinibacillus sphaericus
J. Biol. Chem.
253
5719-5725
1978
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1
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1
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6
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12
1
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16
6
14
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2
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1
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1
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28
1
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349667
Ohshima
-
Leucine dehydrogenase of bacil ...
Lysinibacillus sphaericus
Agric. Biol. Chem.
42
1739-1743
1978
-
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-
-
-
-
2
-
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1
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1
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2
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2
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2
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1
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349666
Obermeier
Some physiological functions o ...
Bacillus subtilis
Arch. Microbiol.
109
59-63
1976
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4
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2
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2
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-
2
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1
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-
2
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-
-
-
-
-
-
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-
-
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349672
Oki
Antitumor activities of bacter ...
Lysinibacillus sphaericus
FEBS Lett.
33
286-288
1973
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-
-
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-
-
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1
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2
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2
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2
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-
2
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349675
Soda
Crystalline L-leucine dehydrog ...
Lysinibacillus sphaericus
Biochem. Biophys. Res. Commun.
44
931-935
1971
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1
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1
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5
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3
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3
1
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2
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1
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1
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5
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349658
Zink
-
L-Leucine dehydrogenase (Bacil ...
Bacillus cereus
Methods Enzymol.
17A
799-802
1970
-
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-
-
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2
7
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1
2
4
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2
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7
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349660
Zink
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The distribution and substrate ...
Bacillus cereus, Priestia megaterium, Bacillus subtilis, Bacillus pumilus, Bacillus mycoides, Bacillus niger, Lysinibacillus sphaericus
Arch. Biochem. Biophys.
99
72-77
1962
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19
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1
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14
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-
14
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14
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1
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4
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19
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1
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349659
Sanwal
L-Leucine dehydrogenase of Bac ...
Bacillus cereus
Arch. Biochem. Biophys.
94
430-435
1961
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5
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1
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1
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2
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1
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4
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4
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2
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5
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-
1
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1
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2
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1
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-
-