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Literature summary for 1.4.1.9 extracted from

  • Ohshima, T.; Misono, H.; Soda, K.
    Properties of crystalline leucine dehydrogenase from Bacillus sphaericus (1978), J. Biol. Chem., 253, 5719-5725.
    View publication on PubMed

Crystallization (Commentary)

Crystallization (Comment) Organism
crystallized by addition of ammonium sulfate Lysinibacillus sphaericus

Inhibitors

Inhibitors Comment Organism Structure
Co2+ CoCl2 Lysinibacillus sphaericus
Cu2+ CuSO4 Lysinibacillus sphaericus
D-2-aminobutanoate
-
Lysinibacillus sphaericus
D-alloisoleucine
-
Lysinibacillus sphaericus
D-Leu
-
Lysinibacillus sphaericus
D-norleucine
-
Lysinibacillus sphaericus
D-Norvaline
-
Lysinibacillus sphaericus
D-Val
-
Lysinibacillus sphaericus
HgCl2
-
Bacillus cereus
HgCl2
-
Lysinibacillus sphaericus
MgCl2
-
Lysinibacillus sphaericus
Na2S
-
Lysinibacillus sphaericus
NAD+ product inhibition Lysinibacillus sphaericus
PCMB
-
Bacillus cereus
PCMB
-
Lysinibacillus sphaericus
pyridoxal 5'-phosphate
-
Lysinibacillus sphaericus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.31
-
4-methyl-2-oxopentanoate
-
Lysinibacillus sphaericus
0.39
-
NAD+
-
Lysinibacillus sphaericus
0.77
-
3-acetylpyridine-NAD+
-
Lysinibacillus sphaericus
1
-
L-Leu
-
Lysinibacillus sphaericus
1.4
-
3-methyl-2-oxobutanoate
-
Lysinibacillus sphaericus
1.7
-
L-Val
-
Lysinibacillus sphaericus
1.7
-
2-oxopentanoate
-
Lysinibacillus sphaericus
1.8
-
L-Ile
-
Lysinibacillus sphaericus
2.41
-
3-acetylpyridine-deamino-NAD+
-
Lysinibacillus sphaericus
3.5
-
L-norvaline
-
Lysinibacillus sphaericus
6.3
-
L-norleucine
-
Lysinibacillus sphaericus
7
-
2-Oxohexanoate
-
Lysinibacillus sphaericus
7.7
-
2-oxobutanoate
-
Lysinibacillus sphaericus
10
-
L-2-aminobutanoate
-
Lysinibacillus sphaericus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
41000
-
6 * 41000, SDS-PAGE Lysinibacillus sphaericus
245000
-
equilibrium sedimentation Lysinibacillus sphaericus

Organism

Organism UniProt Comment Textmining
Bacillus cereus
-
-
-
Bacillus pumilus
-
var. flavus
-
Bacillus subtilis
-
-
-
Brevibacillus brevis
-
-
-
Lysinibacillus sphaericus
-
-
-
Priestia megaterium
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Lysinibacillus sphaericus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
42.3
-
-
Lysinibacillus sphaericus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-2-aminobutanoate + H2O + NAD+
-
Bacillus subtilis 2-oxobutanoate + NH3 + NADH + H+
-
r
L-2-aminobutanoate + H2O + NAD+
-
Bacillus cereus 2-oxobutanoate + NH3 + NADH + H+
-
r
L-2-aminobutanoate + H2O + NAD+ 41% of the activity with L-Leu Lysinibacillus sphaericus 2-oxobutanoate + NH3 + NADH + H+
-
r
L-2-aminobutanoate + H2O + NAD+ 14% of the activity with L-Leu Lysinibacillus sphaericus 2-oxobutanoate + NH3 + NADH + H+
-
r
L-Ile + H2O + NAD+
-
Bacillus subtilis 3-methyl-2-oxopentanoate + NH3 + NADH
-
r
L-Ile + H2O + NAD+
-
Bacillus cereus 3-methyl-2-oxopentanoate + NH3 + NADH
-
r
L-Ile + H2O + NAD+ 58% of the activity with L-Leu Lysinibacillus sphaericus 3-methyl-2-oxopentanoate + NH3 + NADH
-
r
L-Leu + H2O + 3-acetylpyridine-deamino-NAD+ as effective as NAD+ Lysinibacillus sphaericus 4-methyl-2-oxopentanoate + NH3 + 3-acetylpyridine-deamino-NADH
-
?
L-Leu + H2O + 3-acetylpyridine-NAD+ 166% of the activity with NAD+ Lysinibacillus sphaericus 4-methyl-2-oxopentanoate + NH3 + 3-acetylpyridine-NADH
-
?
L-Leu + H2O + 3-pyridinealdehyde-NAD+ 19% of the activity with NAD+ Lysinibacillus sphaericus 4-methyl-2-oxopentanoate + NH3 + 3-pyridinealdehyde-NADH
-
?
L-Leu + H2O + deamino-NAD+ 81% of the activity with NAD+ Lysinibacillus sphaericus 4-methyl-2-oxopentanoate + NH3 + deamino-NADH
-
?
L-Leu + H2O + NAD+
-
Brevibacillus brevis 4-methyl-2-oxopentanoate + NH3 + NADH
-
r
L-Leu + H2O + NAD+
-
Bacillus subtilis 4-methyl-2-oxopentanoate + NH3 + NADH
-
r
L-Leu + H2O + NAD+
-
Priestia megaterium 4-methyl-2-oxopentanoate + NH3 + NADH
-
r
L-Leu + H2O + NAD+
-
Lysinibacillus sphaericus 4-methyl-2-oxopentanoate + NH3 + NADH
-
r
L-Leu + H2O + NAD+
-
Bacillus cereus 4-methyl-2-oxopentanoate + NH3 + NADH
-
r
L-Leu + H2O + NAD+
-
Bacillus pumilus 4-methyl-2-oxopentanoate + NH3 + NADH
-
r
L-Leu + H2O + thionicotinamide-NAD+ 21% of the activity with NAD+ Lysinibacillus sphaericus 4-methyl-2-oxopentanoate + NH3 + thionicotinamide-NADH
-
?
L-norleucine + H2O + NAD+
-
Bacillus subtilis 2-oxohexanoate + NH3 + NADH
-
?
L-norleucine + H2O + NAD+
-
Bacillus cereus 2-oxohexanoate + NH3 + NADH
-
r
L-norleucine + H2O + NAD+ 74% of the activity with L-Leu Lysinibacillus sphaericus 2-oxohexanoate + NH3 + NADH
-
r
L-tert-Leu + H2O + NAD+
-
Lysinibacillus sphaericus 3,3-dimethyl-2-oxobutanoate + NH3 + NADH
-
?
L-Val + H2O + NAD+
-
Bacillus subtilis 3-methyl-2-oxobutanoate + NH3 + NADH
-
?
L-Val + H2O + NAD+
-
Bacillus cereus 3-methyl-2-oxobutanoate + NH3 + NADH
-
?
L-Val + H2O + NAD+ 74% of the activity with L-Leu Lysinibacillus sphaericus 3-methyl-2-oxobutanoate + NH3 + NADH
-
?
additional information the enzyme is B-stereospecific Lysinibacillus sphaericus ?
-
?
additional information reductive amination proceeds through a sequential ordered ternary-binary mechanism Lysinibacillus sphaericus ?
-
?

Subunits

Subunits Comment Organism
hexamer 6 * 41000, SDS-PAGE Lysinibacillus sphaericus

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Brevibacillus brevis
NAD+
-
Bacillus subtilis
NAD+
-
Priestia megaterium
NAD+
-
Lysinibacillus sphaericus
NAD+
-
Bacillus cereus
NAD+
-
Bacillus pumilus
NADH
-
Brevibacillus brevis
NADH
-
Bacillus subtilis
NADH
-
Priestia megaterium
NADH
-
Lysinibacillus sphaericus
NADH
-
Bacillus cereus
NADH
-
Bacillus pumilus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.2
-
D-Leu inhibition of deamination of D-Leu Lysinibacillus sphaericus
3
-
D-Norvaline inhibition of deamination of D-Leu Lysinibacillus sphaericus
7.9
-
D-alloisoleucine inhibition of deamination of D-Leu Lysinibacillus sphaericus
9.4
-
D-2-aminobutanoate inhibition of deamination of D-Leu Lysinibacillus sphaericus
10.8
-
D-Val inhibition of deamination of D-Leu Lysinibacillus sphaericus
52.6
-
D-norleucine inhibition of deamination of D-Leu Lysinibacillus sphaericus