Crystallization (Comment) | Organism |
---|---|
crystallized by addition of ammonium sulfate | Lysinibacillus sphaericus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Co2+ | CoCl2 | Lysinibacillus sphaericus | |
Cu2+ | CuSO4 | Lysinibacillus sphaericus | |
D-2-aminobutanoate | - |
Lysinibacillus sphaericus | |
D-alloisoleucine | - |
Lysinibacillus sphaericus | |
D-Leu | - |
Lysinibacillus sphaericus | |
D-norleucine | - |
Lysinibacillus sphaericus | |
D-Norvaline | - |
Lysinibacillus sphaericus | |
D-Val | - |
Lysinibacillus sphaericus | |
HgCl2 | - |
Bacillus cereus | |
HgCl2 | - |
Lysinibacillus sphaericus | |
MgCl2 | - |
Lysinibacillus sphaericus | |
Na2S | - |
Lysinibacillus sphaericus | |
NAD+ | product inhibition | Lysinibacillus sphaericus | |
PCMB | - |
Bacillus cereus | |
PCMB | - |
Lysinibacillus sphaericus | |
pyridoxal 5'-phosphate | - |
Lysinibacillus sphaericus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.31 | - |
4-methyl-2-oxopentanoate | - |
Lysinibacillus sphaericus | |
0.39 | - |
NAD+ | - |
Lysinibacillus sphaericus | |
0.77 | - |
3-acetylpyridine-NAD+ | - |
Lysinibacillus sphaericus | |
1 | - |
L-Leu | - |
Lysinibacillus sphaericus | |
1.4 | - |
3-methyl-2-oxobutanoate | - |
Lysinibacillus sphaericus | |
1.7 | - |
L-Val | - |
Lysinibacillus sphaericus | |
1.7 | - |
2-oxopentanoate | - |
Lysinibacillus sphaericus | |
1.8 | - |
L-Ile | - |
Lysinibacillus sphaericus | |
2.41 | - |
3-acetylpyridine-deamino-NAD+ | - |
Lysinibacillus sphaericus | |
3.5 | - |
L-norvaline | - |
Lysinibacillus sphaericus | |
6.3 | - |
L-norleucine | - |
Lysinibacillus sphaericus | |
7 | - |
2-Oxohexanoate | - |
Lysinibacillus sphaericus | |
7.7 | - |
2-oxobutanoate | - |
Lysinibacillus sphaericus | |
10 | - |
L-2-aminobutanoate | - |
Lysinibacillus sphaericus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
41000 | - |
6 * 41000, SDS-PAGE | Lysinibacillus sphaericus |
245000 | - |
equilibrium sedimentation | Lysinibacillus sphaericus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus cereus | - |
- |
- |
Bacillus pumilus | - |
var. flavus | - |
Bacillus subtilis | - |
- |
- |
Brevibacillus brevis | - |
- |
- |
Lysinibacillus sphaericus | - |
- |
- |
Priestia megaterium | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Lysinibacillus sphaericus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
42.3 | - |
- |
Lysinibacillus sphaericus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-2-aminobutanoate + H2O + NAD+ | - |
Bacillus subtilis | 2-oxobutanoate + NH3 + NADH + H+ | - |
r | |
L-2-aminobutanoate + H2O + NAD+ | - |
Bacillus cereus | 2-oxobutanoate + NH3 + NADH + H+ | - |
r | |
L-2-aminobutanoate + H2O + NAD+ | 41% of the activity with L-Leu | Lysinibacillus sphaericus | 2-oxobutanoate + NH3 + NADH + H+ | - |
r | |
L-2-aminobutanoate + H2O + NAD+ | 14% of the activity with L-Leu | Lysinibacillus sphaericus | 2-oxobutanoate + NH3 + NADH + H+ | - |
r | |
L-Ile + H2O + NAD+ | - |
Bacillus subtilis | 3-methyl-2-oxopentanoate + NH3 + NADH | - |
r | |
L-Ile + H2O + NAD+ | - |
Bacillus cereus | 3-methyl-2-oxopentanoate + NH3 + NADH | - |
r | |
L-Ile + H2O + NAD+ | 58% of the activity with L-Leu | Lysinibacillus sphaericus | 3-methyl-2-oxopentanoate + NH3 + NADH | - |
r | |
L-Leu + H2O + 3-acetylpyridine-deamino-NAD+ | as effective as NAD+ | Lysinibacillus sphaericus | 4-methyl-2-oxopentanoate + NH3 + 3-acetylpyridine-deamino-NADH | - |
? | |
L-Leu + H2O + 3-acetylpyridine-NAD+ | 166% of the activity with NAD+ | Lysinibacillus sphaericus | 4-methyl-2-oxopentanoate + NH3 + 3-acetylpyridine-NADH | - |
? | |
L-Leu + H2O + 3-pyridinealdehyde-NAD+ | 19% of the activity with NAD+ | Lysinibacillus sphaericus | 4-methyl-2-oxopentanoate + NH3 + 3-pyridinealdehyde-NADH | - |
? | |
L-Leu + H2O + deamino-NAD+ | 81% of the activity with NAD+ | Lysinibacillus sphaericus | 4-methyl-2-oxopentanoate + NH3 + deamino-NADH | - |
? | |
L-Leu + H2O + NAD+ | - |
Brevibacillus brevis | 4-methyl-2-oxopentanoate + NH3 + NADH | - |
r | |
L-Leu + H2O + NAD+ | - |
Bacillus subtilis | 4-methyl-2-oxopentanoate + NH3 + NADH | - |
r | |
L-Leu + H2O + NAD+ | - |
Priestia megaterium | 4-methyl-2-oxopentanoate + NH3 + NADH | - |
r | |
L-Leu + H2O + NAD+ | - |
Lysinibacillus sphaericus | 4-methyl-2-oxopentanoate + NH3 + NADH | - |
r | |
L-Leu + H2O + NAD+ | - |
Bacillus cereus | 4-methyl-2-oxopentanoate + NH3 + NADH | - |
r | |
L-Leu + H2O + NAD+ | - |
Bacillus pumilus | 4-methyl-2-oxopentanoate + NH3 + NADH | - |
r | |
L-Leu + H2O + thionicotinamide-NAD+ | 21% of the activity with NAD+ | Lysinibacillus sphaericus | 4-methyl-2-oxopentanoate + NH3 + thionicotinamide-NADH | - |
? | |
L-norleucine + H2O + NAD+ | - |
Bacillus subtilis | 2-oxohexanoate + NH3 + NADH | - |
? | |
L-norleucine + H2O + NAD+ | - |
Bacillus cereus | 2-oxohexanoate + NH3 + NADH | - |
r | |
L-norleucine + H2O + NAD+ | 74% of the activity with L-Leu | Lysinibacillus sphaericus | 2-oxohexanoate + NH3 + NADH | - |
r | |
L-tert-Leu + H2O + NAD+ | - |
Lysinibacillus sphaericus | 3,3-dimethyl-2-oxobutanoate + NH3 + NADH | - |
? | |
L-Val + H2O + NAD+ | - |
Bacillus subtilis | 3-methyl-2-oxobutanoate + NH3 + NADH | - |
? | |
L-Val + H2O + NAD+ | - |
Bacillus cereus | 3-methyl-2-oxobutanoate + NH3 + NADH | - |
? | |
L-Val + H2O + NAD+ | 74% of the activity with L-Leu | Lysinibacillus sphaericus | 3-methyl-2-oxobutanoate + NH3 + NADH | - |
? | |
additional information | the enzyme is B-stereospecific | Lysinibacillus sphaericus | ? | - |
? | |
additional information | reductive amination proceeds through a sequential ordered ternary-binary mechanism | Lysinibacillus sphaericus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
hexamer | 6 * 41000, SDS-PAGE | Lysinibacillus sphaericus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Brevibacillus brevis | |
NAD+ | - |
Bacillus subtilis | |
NAD+ | - |
Priestia megaterium | |
NAD+ | - |
Lysinibacillus sphaericus | |
NAD+ | - |
Bacillus cereus | |
NAD+ | - |
Bacillus pumilus | |
NADH | - |
Brevibacillus brevis | |
NADH | - |
Bacillus subtilis | |
NADH | - |
Priestia megaterium | |
NADH | - |
Lysinibacillus sphaericus | |
NADH | - |
Bacillus cereus | |
NADH | - |
Bacillus pumilus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
2.2 | - |
D-Leu | inhibition of deamination of D-Leu | Lysinibacillus sphaericus | |
3 | - |
D-Norvaline | inhibition of deamination of D-Leu | Lysinibacillus sphaericus | |
7.9 | - |
D-alloisoleucine | inhibition of deamination of D-Leu | Lysinibacillus sphaericus | |
9.4 | - |
D-2-aminobutanoate | inhibition of deamination of D-Leu | Lysinibacillus sphaericus | |
10.8 | - |
D-Val | inhibition of deamination of D-Leu | Lysinibacillus sphaericus | |
52.6 | - |
D-norleucine | inhibition of deamination of D-Leu | Lysinibacillus sphaericus |