Inhibitors | Comment | Organism | Structure |
---|---|---|---|
HgCl2 | - |
[Bacillus] caldolyticus | |
p-mercuribenzoate | - |
[Bacillus] caldolyticus | |
pyridoxal 5'-phosphate | - |
[Bacillus] caldolyticus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.036 | - |
NADH | - |
[Bacillus] caldolyticus | |
0.18 | - |
NAD+ | - |
[Bacillus] caldolyticus | |
0.7 | - |
4-methyl-2-oxopentanoate | - |
[Bacillus] caldolyticus | |
0.7 | - |
L-Ile | - |
[Bacillus] caldolyticus | |
0.81 | - |
2-oxopentanoate | - |
[Bacillus] caldolyticus | |
1.1 | - |
L-Leu | - |
[Bacillus] caldolyticus | |
1.8 | - |
L-tert-Leu | - |
[Bacillus] caldolyticus | |
1.9 | - |
2-oxo-3-methylpentanoate | - |
[Bacillus] caldolyticus | |
2 | - |
L-Val | - |
[Bacillus] caldolyticus | |
2.1 | - |
L-norleucine | - |
[Bacillus] caldolyticus | |
2.3 | - |
L-norvaline | - |
[Bacillus] caldolyticus | |
2.4 | - |
3-methyl-2-oxobutanoate | - |
[Bacillus] caldolyticus | |
2.7 | - |
2-Oxohexanoate | - |
[Bacillus] caldolyticus | |
6.9 | - |
2-oxobutanoate | - |
[Bacillus] caldolyticus | |
8.1 | - |
4-(methylsulfanyl)-2-oxobutanoate | - |
[Bacillus] caldolyticus | |
8.9 | - |
L-2-aminobutanoate | - |
[Bacillus] caldolyticus | |
19 | - |
2-oxo-3,3-dimethylbutanoate | - |
[Bacillus] caldolyticus | |
23 | - |
L-Met | - |
[Bacillus] caldolyticus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
39000 | - |
8 * 39000, SDS-PAGE | [Bacillus] caldolyticus |
313000 | - |
non-denaturing PAGE | [Bacillus] caldolyticus |
325000 | - |
gel filtration | [Bacillus] caldolyticus |
Organic Solvent | Comment | Organism |
---|---|---|
2-propanol | 2 M, 4°C, 5% loss of activity after 2 months | [Bacillus] caldolyticus |
acetonitrile | 2 M, 4°C, 22% loss of activity after 3 d | [Bacillus] caldolyticus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
[Bacillus] caldolyticus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
[Bacillus] caldolyticus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
[Bacillus] caldolyticus |
Storage Stability | Organism |
---|---|
4°C, 50 mM potassium phosphate, 1 mM dithioerythritol, pH 7.5, 15% loss of activity after 2 months | [Bacillus] caldolyticus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxo-3,3-dimethylbutanoate + NH3 + NADH | - |
[Bacillus] caldolyticus | L-2-amino-3,3-dimethylbutanoate + NAD+ + H2O | - |
? | |
4-(methylsulfanyl)-2-oxobutanoate + NH3 + NADH + H+ | - |
[Bacillus] caldolyticus | L-methionine + H2O + NAD+ | - |
r | |
L-2-aminobutanoate + H2O + NAD+ | - |
[Bacillus] caldolyticus | 2-oxobutanoate + NH3 + NADH + H+ | - |
r | |
L-Ile + H2O + NAD+ | - |
[Bacillus] caldolyticus | 3-methyl-2-oxopentanoate + NH3 + NADH | - |
r | |
L-Leu + H2O + NAD+ | - |
[Bacillus] caldolyticus | 4-methyl-2-oxopentanoate + NH3 + NADH | - |
r | |
L-Met + H2O + NAD+ | - |
[Bacillus] caldolyticus | 4-methylthio-2-oxobutyrate + NH3 + NADH + H+ | - |
? | |
L-norleucine + H2O + NAD+ | - |
[Bacillus] caldolyticus | 2-oxohexanoate + NH3 + NADH | - |
r | |
L-norvaline + H2O + NAD+ | - |
[Bacillus] caldolyticus | 2-oxopentanoate + NH3 + NADH + H+ | - |
r | |
L-tert-Leu + H2O + NAD+ | - |
[Bacillus] caldolyticus | 3,3-dimethyl-2-oxobutanoate + NH3 + NADH | - |
? | |
L-Val + H2O + NAD+ | - |
[Bacillus] caldolyticus | 3-methyl-2-oxobutanoate + NH3 + NADH | - |
r |
Subunits | Comment | Organism |
---|---|---|
octamer | 8 * 39000, SDS-PAGE | [Bacillus] caldolyticus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
pH 7.5, 30 min, 50% loss of activity | [Bacillus] caldolyticus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6.5 | 10.5 | 30 min, 50% loss of activity | [Bacillus] caldolyticus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
[Bacillus] caldolyticus | |
NADH | - |
[Bacillus] caldolyticus |