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Literature summary for 1.4.1.9 extracted from

  • Kaerst, U.; Schuette, H.; Baydoun, H.; Tsai, H.
    Purification and characterization of leucine dehydrogenase from the thermophile Bacillus caldolyticus (1989), J. Gen. Microbiol., 135, 1305-1313.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
HgCl2
-
[Bacillus] caldolyticus
p-mercuribenzoate
-
[Bacillus] caldolyticus
pyridoxal 5'-phosphate
-
[Bacillus] caldolyticus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.036
-
NADH
-
[Bacillus] caldolyticus
0.18
-
NAD+
-
[Bacillus] caldolyticus
0.7
-
4-methyl-2-oxopentanoate
-
[Bacillus] caldolyticus
0.7
-
L-Ile
-
[Bacillus] caldolyticus
0.81
-
2-oxopentanoate
-
[Bacillus] caldolyticus
1.1
-
L-Leu
-
[Bacillus] caldolyticus
1.8
-
L-tert-Leu
-
[Bacillus] caldolyticus
1.9
-
2-oxo-3-methylpentanoate
-
[Bacillus] caldolyticus
2
-
L-Val
-
[Bacillus] caldolyticus
2.1
-
L-norleucine
-
[Bacillus] caldolyticus
2.3
-
L-norvaline
-
[Bacillus] caldolyticus
2.4
-
3-methyl-2-oxobutanoate
-
[Bacillus] caldolyticus
2.7
-
2-Oxohexanoate
-
[Bacillus] caldolyticus
6.9
-
2-oxobutanoate
-
[Bacillus] caldolyticus
8.1
-
4-(methylsulfanyl)-2-oxobutanoate
-
[Bacillus] caldolyticus
8.9
-
L-2-aminobutanoate
-
[Bacillus] caldolyticus
19
-
2-oxo-3,3-dimethylbutanoate
-
[Bacillus] caldolyticus
23
-
L-Met
-
[Bacillus] caldolyticus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
39000
-
8 * 39000, SDS-PAGE [Bacillus] caldolyticus
313000
-
non-denaturing PAGE [Bacillus] caldolyticus
325000
-
gel filtration [Bacillus] caldolyticus

Organic Solvent Stability

Organic Solvent Comment Organism
2-propanol 2 M, 4°C, 5% loss of activity after 2 months [Bacillus] caldolyticus
acetonitrile 2 M, 4°C, 22% loss of activity after 3 d [Bacillus] caldolyticus

Organism

Organism UniProt Comment Textmining
[Bacillus] caldolyticus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
[Bacillus] caldolyticus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
[Bacillus] caldolyticus

Storage Stability

Storage Stability Organism
4°C, 50 mM potassium phosphate, 1 mM dithioerythritol, pH 7.5, 15% loss of activity after 2 months [Bacillus] caldolyticus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-oxo-3,3-dimethylbutanoate + NH3 + NADH
-
[Bacillus] caldolyticus L-2-amino-3,3-dimethylbutanoate + NAD+ + H2O
-
?
4-(methylsulfanyl)-2-oxobutanoate + NH3 + NADH + H+
-
[Bacillus] caldolyticus L-methionine + H2O + NAD+
-
r
L-2-aminobutanoate + H2O + NAD+
-
[Bacillus] caldolyticus 2-oxobutanoate + NH3 + NADH + H+
-
r
L-Ile + H2O + NAD+
-
[Bacillus] caldolyticus 3-methyl-2-oxopentanoate + NH3 + NADH
-
r
L-Leu + H2O + NAD+
-
[Bacillus] caldolyticus 4-methyl-2-oxopentanoate + NH3 + NADH
-
r
L-Met + H2O + NAD+
-
[Bacillus] caldolyticus 4-methylthio-2-oxobutyrate + NH3 + NADH + H+
-
?
L-norleucine + H2O + NAD+
-
[Bacillus] caldolyticus 2-oxohexanoate + NH3 + NADH
-
r
L-norvaline + H2O + NAD+
-
[Bacillus] caldolyticus 2-oxopentanoate + NH3 + NADH + H+
-
r
L-tert-Leu + H2O + NAD+
-
[Bacillus] caldolyticus 3,3-dimethyl-2-oxobutanoate + NH3 + NADH
-
?
L-Val + H2O + NAD+
-
[Bacillus] caldolyticus 3-methyl-2-oxobutanoate + NH3 + NADH
-
r

Subunits

Subunits Comment Organism
octamer 8 * 39000, SDS-PAGE [Bacillus] caldolyticus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
80
-
pH 7.5, 30 min, 50% loss of activity [Bacillus] caldolyticus

pH Stability

pH Stability pH Stability Maximum Comment Organism
6.5 10.5 30 min, 50% loss of activity [Bacillus] caldolyticus

Cofactor

Cofactor Comment Organism Structure
NAD+
-
[Bacillus] caldolyticus
NADH
-
[Bacillus] caldolyticus