Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu(CH3COO)2 | 1 mM, 20% inhibition | Geobacillus stearothermophilus | |
Cu2+ | 1 mM CuCl2, 22% inhibition | Geobacillus stearothermophilus | |
HgCl2 | 0.01 M, 65% inhibition | Geobacillus stearothermophilus | |
Pb(CH3COO)2 | 1 mM, 20% inhibition | Geobacillus stearothermophilus | |
PCMB | 0.01 M, 50% inhibition | Geobacillus stearothermophilus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
49000 | - |
6 * 49000, SDS-PAGE | Geobacillus stearothermophilus |
300000 | - |
gel filtration | Geobacillus stearothermophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus stearothermophilus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Geobacillus stearothermophilus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
120 | - |
- |
Geobacillus stearothermophilus |
Storage Stability | Organism |
---|---|
4°C, stable for at least 1 month | Geobacillus stearothermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-Ile + H2O + NAD+ | 73% of the activity with L-Leu | Geobacillus stearothermophilus | 3-methyl-2-oxopentanoate + NH3 + NADH | - |
r | |
L-Leu + H2O + NAD+ | - |
Geobacillus stearothermophilus | 4-methyl-2-oxopentanoate + NH3 + NADH | - |
r | |
L-Met + H2O + NAD+ | no activity | Geobacillus stearothermophilus | 4-methylthio-2-oxobutyrate + NH3 + NADH + H+ | - |
? | |
L-Val + H2O + NAD+ | 98% of the activity with L-Leu | Geobacillus stearothermophilus | 3-methyl-2-oxobutanoate + NH3 + NADH | - |
r |
Subunits | Comment | Organism |
---|---|---|
hexamer | 6 * 49000, SDS-PAGE | Geobacillus stearothermophilus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
68 | 70 | oxidative amination of L-Leu | Geobacillus stearothermophilus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | 80 | 50°C: 37% of maximal activity, 80°C: 55% of maximal activity, oxidative amination of L-Leu | Geobacillus stearothermophilus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
20 min, enzyme retains full activity | Geobacillus stearothermophilus |
80 | - |
5 min, substantial loss of activity | Geobacillus stearothermophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.8 | - |
reductive amination of 3-methyl-2-oxobutanoate | Geobacillus stearothermophilus |
9.7 | - |
reductive amination of 4-methyl-2-oxopentanoate | Geobacillus stearothermophilus |
11 | - |
oxidative deamination of L-Leu | Geobacillus stearothermophilus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5.5 | 10 | 55°C, 5 min, stable | Geobacillus stearothermophilus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | no activity with NADPH | Geobacillus stearothermophilus | |
additional information | no activity with NADP+ | Geobacillus stearothermophilus | |
NAD+ | - |
Geobacillus stearothermophilus | |
NADH | - |
Geobacillus stearothermophilus |