Crystallization (Comment) | Organism |
---|---|
- |
Bacillus cereus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
HgCl2 | 1 mM, 92% inhibition | Bacillus cereus | |
KCN | 1 mM, 43% inhibition | Bacillus cereus | |
p-mercuribenzoate | 1 mM, 75% inhibition | Bacillus cereus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.034 | - |
NADH | - |
Bacillus cereus | |
0.34 | - |
NAD+ | - |
Bacillus cereus | |
0.4 | - |
2-oxopentanoate | - |
Bacillus cereus | |
0.45 | - |
4-methyl-2-oxopentanoate | - |
Bacillus cereus | |
0.9 | - |
2-oxo-3-methylpentanoate | - |
Bacillus cereus | |
1 | - |
L-Ile | - |
Bacillus cereus | |
1.2 | - |
2-Oxohexanoate | - |
Bacillus cereus | |
1.5 | - |
2-oxobutanoate | - |
Bacillus cereus | |
1.5 | - |
L-Leu | - |
Bacillus cereus | |
1.5 | - |
L-norleucine | - |
Bacillus cereus | |
2.1 | - |
3-methyl-2-oxobutanoate | - |
Bacillus cereus | |
2.1 | - |
3-methylthio-2-oxobutanoate | - |
Bacillus cereus | |
2.5 | - |
L-Val | - |
Bacillus cereus | |
2.9 | - |
L-norvaline | - |
Bacillus cereus | |
22 | - |
L-2-aminobutanoate | - |
Bacillus cereus | |
23 | - |
L-Met | - |
Bacillus cereus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
39000 | - |
8 * 39000, SDS-PAGE | Bacillus cereus |
310000 | - |
gel filtration, equilibrium sedimenation | Bacillus cereus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus cereus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Bacillus cereus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
57 | - |
- |
Bacillus cereus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-2-aminobutanoate + H2O + NAD+ | - |
Bacillus cereus | 2-oxobutanoate + NH3 + NADH + H+ | - |
r | |
L-Ile + H2O + NAD+ | - |
Bacillus cereus | 3-methyl-2-oxopentanoate + NH3 + NADH | - |
r | |
L-Leu + H2O + NAD+ | - |
Bacillus cereus | 4-methyl-2-oxopentanoate + NH3 + NADH | - |
r | |
L-Met + H2O + NAD+ | - |
Bacillus cereus | 4-methylthio-2-oxobutyrate + NH3 + NADH + H+ | - |
r | |
L-norleucine + H2O + NAD+ | - |
Bacillus cereus | 2-oxohexanoate + NH3 + NADH | - |
r | |
L-norvaline + H2O + NAD+ | - |
Bacillus cereus | 2-oxopentanoate + NH3 + NADH + H+ | - |
r | |
L-Val + H2O + NAD+ | - |
Bacillus cereus | 3-methyl-2-oxobutanoate + NH3 + NADH | - |
r |
Subunits | Comment | Organism |
---|---|---|
octamer | 8 * 39000, SDS-PAGE | Bacillus cereus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
reductive amination of 2-oxopentanoate and 3-methyl-2-oxobutanoate | Bacillus cereus |
9 | 9.5 | reductive amination of 4-methyl-2-oxopentanoate, 2-oxopentanoate and 2-oxomethionine | Bacillus cereus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5.6 | 9.8 | 25°C, 24 h, maximal loss of 12% of the activity | Bacillus cereus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | no activity with NADPH | Bacillus cereus | |
additional information | no activity with NADP+ | Bacillus cereus | |
NAD+ | - |
Bacillus cereus | |
NADH | - |
Bacillus cereus |