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Literature summary for 1.4.1.9 extracted from

  • Schuette, H.; Hummel, W.; Tsai, H.; Kula, M.R.
    L-Leucine dehydrogenase from Bacillus cereus (1985), Appl. Microbiol. Biotechnol., 22, 306-317.
No PubMed abstract available

Crystallization (Commentary)

Crystallization (Comment) Organism
-
Bacillus cereus

Inhibitors

Inhibitors Comment Organism Structure
HgCl2 1 mM, 92% inhibition Bacillus cereus
KCN 1 mM, 43% inhibition Bacillus cereus
p-mercuribenzoate 1 mM, 75% inhibition Bacillus cereus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.034
-
NADH
-
Bacillus cereus
0.34
-
NAD+
-
Bacillus cereus
0.4
-
2-oxopentanoate
-
Bacillus cereus
0.45
-
4-methyl-2-oxopentanoate
-
Bacillus cereus
0.9
-
2-oxo-3-methylpentanoate
-
Bacillus cereus
1
-
L-Ile
-
Bacillus cereus
1.2
-
2-Oxohexanoate
-
Bacillus cereus
1.5
-
2-oxobutanoate
-
Bacillus cereus
1.5
-
L-Leu
-
Bacillus cereus
1.5
-
L-norleucine
-
Bacillus cereus
2.1
-
3-methyl-2-oxobutanoate
-
Bacillus cereus
2.1
-
3-methylthio-2-oxobutanoate
-
Bacillus cereus
2.5
-
L-Val
-
Bacillus cereus
2.9
-
L-norvaline
-
Bacillus cereus
22
-
L-2-aminobutanoate
-
Bacillus cereus
23
-
L-Met
-
Bacillus cereus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
39000
-
8 * 39000, SDS-PAGE Bacillus cereus
310000
-
gel filtration, equilibrium sedimenation Bacillus cereus

Organism

Organism UniProt Comment Textmining
Bacillus cereus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Bacillus cereus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
57
-
-
Bacillus cereus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-2-aminobutanoate + H2O + NAD+
-
Bacillus cereus 2-oxobutanoate + NH3 + NADH + H+
-
r
L-Ile + H2O + NAD+
-
Bacillus cereus 3-methyl-2-oxopentanoate + NH3 + NADH
-
r
L-Leu + H2O + NAD+
-
Bacillus cereus 4-methyl-2-oxopentanoate + NH3 + NADH
-
r
L-Met + H2O + NAD+
-
Bacillus cereus 4-methylthio-2-oxobutyrate + NH3 + NADH + H+
-
r
L-norleucine + H2O + NAD+
-
Bacillus cereus 2-oxohexanoate + NH3 + NADH
-
r
L-norvaline + H2O + NAD+
-
Bacillus cereus 2-oxopentanoate + NH3 + NADH + H+
-
r
L-Val + H2O + NAD+
-
Bacillus cereus 3-methyl-2-oxobutanoate + NH3 + NADH
-
r

Subunits

Subunits Comment Organism
octamer 8 * 39000, SDS-PAGE Bacillus cereus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
reductive amination of 2-oxopentanoate and 3-methyl-2-oxobutanoate Bacillus cereus
9 9.5 reductive amination of 4-methyl-2-oxopentanoate, 2-oxopentanoate and 2-oxomethionine Bacillus cereus

pH Stability

pH Stability pH Stability Maximum Comment Organism
5.6 9.8 25°C, 24 h, maximal loss of 12% of the activity Bacillus cereus

Cofactor

Cofactor Comment Organism Structure
additional information no activity with NADPH Bacillus cereus
additional information no activity with NADP+ Bacillus cereus
NAD+
-
Bacillus cereus
NADH
-
Bacillus cereus