BRENDA - Enzyme Database
show all sequences of 1.4.1.9

L-Leucine dehydrogenase of Bacillus cereus

Sanwal, B.D.; Zink, M.W.; Arch. Biochem. Biophys. 94, 430-435 (1961)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
PCMB
reversed by L-Cys
Bacillus cereus
Sulfide
Na2S
Bacillus cereus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.1
-
NADH
-
Bacillus cereus
0.16
-
NAD+
-
Bacillus cereus
2.2
-
3-methyl-2-oxobutanoate
-
Bacillus cereus
6.2
-
L-Leu
-
Bacillus cereus
13
-
NH4+
-
Bacillus cereus
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Bacillus cereus
-
-
-
Purification (Commentary)
Commentary
Organism
-
Bacillus cereus
Specific Activity [micromol/min/mg]
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
additional information
-
-
Bacillus cereus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
L-Leu + H2O + NAD+
the equilibrium favors synthesis of L-Leu
349659
Bacillus cereus
4-methyl-2-oxopentanoate + NH3 + NADH
-
349659
Bacillus cereus
r
L-Val + H2O + NAD+
-
349659
Bacillus cereus
3-methyl-2-oxobutanoate + NH3 + NADH
-
-
-
-
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
11.3
-
oxidative deamination
Bacillus cereus
Cofactor
Cofactor
Commentary
Organism
Structure
additional information
no activity with NADP+; no activity with NADPH
Bacillus cereus
NAD+
-
Bacillus cereus
NADH
-
Bacillus cereus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
additional information
no activity with NADP+; no activity with NADPH
Bacillus cereus
NAD+
-
Bacillus cereus
NADH
-
Bacillus cereus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
PCMB
reversed by L-Cys
Bacillus cereus
Sulfide
Na2S
Bacillus cereus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.1
-
NADH
-
Bacillus cereus
0.16
-
NAD+
-
Bacillus cereus
2.2
-
3-methyl-2-oxobutanoate
-
Bacillus cereus
6.2
-
L-Leu
-
Bacillus cereus
13
-
NH4+
-
Bacillus cereus
Purification (Commentary) (protein specific)
Commentary
Organism
-
Bacillus cereus
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
additional information
-
-
Bacillus cereus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
L-Leu + H2O + NAD+
the equilibrium favors synthesis of L-Leu
349659
Bacillus cereus
4-methyl-2-oxopentanoate + NH3 + NADH
-
349659
Bacillus cereus
r
L-Val + H2O + NAD+
-
349659
Bacillus cereus
3-methyl-2-oxobutanoate + NH3 + NADH
-
-
-
-
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
11.3
-
oxidative deamination
Bacillus cereus
Other publictions for EC 1.4.1.9
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [░C]
Temperature Range [░C]
Temperature Stability [░C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [░C] (protein specific)
Temperature Range [░C] (protein specific)
Temperature Stability [░C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
742376
Zhang
-
Engineering bi-functional enz ...
Bacillus cereus, Bacillus cereus ATCC 10987
Eng. Life Sci.
17
989-996
2017
-
1
1
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8
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2
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1
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1
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1
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1
1
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8
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1
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2
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1
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1
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-
-
-
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741652
Lu
The development of leucine de ...
Bacillus cereus
Appl. Biochem. Biotechnol.
180
1180-1195
2016
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1
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4
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1
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4
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1
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4
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1
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-
-
4
-
-
-
-
-
-
-
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4
4
741659
Xu
Semi-rational engineering of ...
Bacillus cereus
Appl. Biochem. Biotechnol.
182
898-909
2016
-
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1
7
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4
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1
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4
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2
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1
7
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4
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4
-
2
-
-
-
-
4
-
-
-
-
-
-
-
-
4
4
741718
Zhu
Directed evolution of leucine ...
Lysinibacillus sphaericus
Appl. Microbiol. Biotechnol.
100
5805-5813
2016
-
1
-
-
1
-
-
4
-
-
-
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2
-
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1
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4
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1
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1
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4
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-
1
-
-
-
-
4
-
-
-
-
-
-
-
-
4
4
742176
Zhu
Expression, purification and ...
Laceyella sacchari
Biotechnol. Lett.
38
855-861
2016
-
-
-
-
-
-
2
2
-
-
-
-
-
3
-
-
-
-
-
-
4
-
4
2
1
-
2
-
1
1
1
-
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-
-
-
-
-
-
-
2
-
2
-
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-
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-
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4
-
4
2
1
-
2
-
1
1
1
-
-
-
-
-
-
-
742375
Jiang
-
A cold-adapted leucine dehydr ...
Alcanivorax dieselolei
Eng. Life Sci.
16
283-289
2016
-
-
-
-
-
-
-
2
-
-
-
-
-
1
-
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1
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1
1
1
1
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2
1
-
1
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2
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1
-
1
1
1
1
-
2
1
-
1
-
-
-
-
-
2
2
741837
Liu
-
Efficient synthesis of L-tert ...
Lysinibacillus sphaericus, Lysinibacillus sphaericus CGMCC 1.1677
Biochem. Eng. J.
91
204-209
2014
-
1
1
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
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2
-
1
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1
1
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-
2
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1
-
-
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-
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-
-
-
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742169
Tao
A one-pot system for producti ...
Bacillus cereus, Bacillus cereus DSM 31
Biotechnol. Lett.
36
835-841
2014
-
1
1
-
-
-
-
-
-
-
-
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5
-
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-
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1
1
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-
743093
Li
-
Stereoselective synthesis of ...
Exiguobacterium sibiricum
J. Mol. Catal. B
105
11-17
2014
-
1
1
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4
4
-
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1
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8
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2
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1
1
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4
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4
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8
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2
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742711
Mahdizadehdehosta
Isolation and characterizatio ...
Citrobacter freundii, Citrobacter freundii JK-91
Iran. J. Microbiol.
5
278-284
2013
-
-
-
-
-
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1
1
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1
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4
-
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1
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1
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4
1
1
1
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1
1
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3
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3
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1
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1
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1
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1
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1
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4
1
1
1
-
-
1
1
-
-
-
-
-
-
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-
725737
Zhao
-
A psychrophilic leucine dehydr ...
Sporosarcina psychrophila, Sporosarcina psychrophila DSM 3
J. Mol. Catal. B
83
65-72
2012
-
-
-
-
-
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2
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-
2
2
-
2
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1
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2
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2
1
1
1
4
1
2
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1
2
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2
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2
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2
2
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1
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2
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2
1
1
1
4
1
2
-
1
-
-
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-
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-
-
726091
Parker
-
Preparation of (S)-1-cycloprop ...
Thermoactinomyces intermedius
Org. Process Res. Dev.
16
464-469
2012
-
1
1
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-
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-
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2
-
1
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1
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3
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1
1
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2
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1
1
2
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2
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1
-
3
-
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1
1
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693504
Chiriac
-
Stereoselective synthesis of L ...
Geobacillus stearothermophilus
J. Labelled Compd. Radiopharm.
51
171-174
2008
-
1
1
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1
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5
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1
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1
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1
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1
1
1
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5
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1
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1
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695691
Li
Cloning, protein sequence clar ...
Lysinibacillus sphaericus, Lysinibacillus sphaericus ATCC 4525
Appl. Biochem. Biotechnol.
158
343-351
2008
-
-
1
-
-
1
-
7
-
-
-
-
-
4
-
-
1
-
-
-
2
1
19
-
-
-
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7
-
-
-
1
-
-
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1
1
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-
1
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7
-
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-
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1
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2
1
19
-
-
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7
-
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-
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656565
Katoh
-
Purification and characterizat ...
Natrialba magadii, Natrialba magadii MS-3
J. Mol. Catal. B
23
231-238
2003
-
-
-
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1
3
7
-
4
2
-
-
2
-
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1
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-
1
1
16
1
1
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2
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1
2
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2
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2
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1
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3
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7
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4
2
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1
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1
1
16
1
1
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2
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1
2
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656566
Katoh
-
Cloning and sequencing of the ...
Lysinibacillus sphaericus
J. Mol. Catal. B
23
239-247
2003
-
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1
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1
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6
-
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3
-
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1
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1
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1
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1
2
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2
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2
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2
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1
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1
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6
-
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3
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1
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1
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1
2
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2
-
2
-
2
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656569
Kataoka
-
Alteration of substrate specif ...
Geobacillus stearothermophilus
J. Mol. Catal. B
23
299-309
2003
-
-
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2
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31
-
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1
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1
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13
-
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25
-
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2
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2
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2
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31
-
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1
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13
-
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25
-
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-
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349692
Muranova
Crystallization and preliminar ...
Lysinibacillus sphaericus, Thermoactinomyces intermedius
Acta Crystallogr. Sect. D
58
1059-1062
2002
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-
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1
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3
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1
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2
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349694
Oikawa
Fragmentary form of thermostab ...
Geobacillus stearothermophilus
Biochem. Biophys. Res. Commun.
280
1177-1182
2001
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1
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3
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1
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1
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1
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1
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349684
Ansorge
Production of recombinant L-le ...
Bacillus cereus
Biotechnol. Bioeng.
68
557-562
2000
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1
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4
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1
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1
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2
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1
2
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1
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1
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349686
Galkin
Construction of a new leucine ...
Thermoactinomyces intermedius
Protein Eng.
10
687-690
1997
-
-
-
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3
-
-
2
-
-
-
-
-
2
-
-
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-
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-
-
2
-
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-
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2
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3
-
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3
-
3
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2
-
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2
-
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2
-
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349698
Morishita
Kinetic assay of serum and uri ...
Bacillus subtilis
Clin. Chem.
43
1932-11936
1997
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1
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2
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1
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2
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1
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2
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-
1
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349689
Kiba
-
Postcolumn co-immobilized leuc ...
Geobacillus stearothermophilus
J. Chromatogr. A
724
354-357
1996
-
1
-
-
-
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-
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1
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349682
Nagata
Gene cloning, purification, an ...
Bacillus licheniformis
Appl. Microbiol. Biotechnol.
44
432-438
1995
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1
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1
7
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2
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3
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1
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7
1
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3
-
3
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3
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1
3
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1
-
7
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2
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1
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7
1
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3
-
3
-
-
-
-
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-
349688
Baker
A role for quaternary structur ...
Lysinibacillus sphaericus
Structure
3
693-705
1995
-
-
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2
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1
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349647
Kataoka
Construction and characterizat ...
Geobacillus stearothermophilus
J. Biochem.
116
931-936
1994
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-
1
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1
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1
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1
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2
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2
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1
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1
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2
1
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2
-
1
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-
349685
Ohshima
The purification, characteriza ...
Thermoactinomyces intermedius
Eur. J. Biochem.
222
305-312
1994
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1
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13
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2
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1
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2
1
6
1
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3
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5
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2
2
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1
2
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13
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2
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1
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2
1
6
1
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3
-
5
-
2
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-
349687
Sekimoto
Role of the conserved glycyl r ...
Geobacillus stearothermophilus
J. Biochem.
116
176-182
1994
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3
1
2
20
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2
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1
1
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1
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4
1
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2
3
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2
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3
1
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2
3
20
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1
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1
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4
1
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-
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-
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349690
Sekimoto
Involvement of conserved lysin ...
Geobacillus stearothermophilus
J. Biol. Chem.
269
7262-7266
1994
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2
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1
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1
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1
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2
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2
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12
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1
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1
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1
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349693
Turnbull
Crystallization and quaternary ...
Lysinibacillus sphaericus
J. Mol. Biol.
236
663-665
1994
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1
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1
-
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349691
Sekimoto
Evidence for lysine 80 as gene ...
Geobacillus stearothermophilus
J. Biol. Chem.
268
27039-27045
1993
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-
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3
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2
16
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2
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1
-
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1
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3
1
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1
2
3
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-
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-
2
-
3
-
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2
3
16
-
-
-
-
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1
-
-
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-
1
-
-
-
3
1
-
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1
-
-
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-
-
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658257
Bradshaw
-
Enzyme-catalyzed asymetric syn ...
Lysinibacillus sphaericus
Bioorg. Chem.
19
29-39
1991
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1
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1
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1
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1
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1
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1
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-
1
-
1
-
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-
1
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349681
Nagata
-
Gene cloning, purification, an ...
Bacillus sp. (in: Bacteria)
J. Ferment. Bioeng.
69
199-203
1990
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3
1
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2
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2
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1
3
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1
3
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2
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-
2
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1
-
3
1
-
-
2
-
2
-
1
-
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-
-
-
349695
Gu
Production of essential L-bran ...
Bacillus sp. (in: Bacteria)
Biotechnol. Bioeng.
36
263-269
1990
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1
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3
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1
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1
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1
-
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-
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349696
Gu
Conversion of ammonia or urea ...
Bacillus sp. (in: Bacteria)
Biotechnol. Appl. Biochem.
12
227-236
1990
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1
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1
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1
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2
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-
1
-
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349664
Kaerst
-
Purification and characterizat ...
Bacillus caldolyticus
J. Gen. Microbiol.
135
1305-1313
1989
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3
18
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3
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2
1
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1
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1
1
9
1
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1
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1
2
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2
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3
-
18
-
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3
-
2
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1
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1
1
9
1
-
-
1
-
-
-
1
-
-
-
-
-
-
-
349670
Schustolla
-
Gene cloning and sequence dete ...
Bacillus cereus
DECHEMA Biotechnol. Conf.
3
1097-1101
1989
-
-
-
-
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1
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1
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1
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2
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2
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1
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1
-
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-
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-
-
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-
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349674
Oka
Overproduction of thermostable ...
Geobacillus stearothermophilus
Biotechnol. Appl. Biochem.
11
307-311
1989
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1
-
-
-
-
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3
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1
-
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1
1
1
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2
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1
2
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-
-
1
2
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-
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1
-
-
1
1
1
-
-
-
2
-
-
-
1
-
-
-
-
-
-
-
349676
Esaki
-
Enantioselective synthesis of ...
Geobacillus stearothermophilus
Biotechnol. Appl. Biochem.
11
312-317
1989
-
1
-
-
-
-
-
1
-
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1
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2
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1
1
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2
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1
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2
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1
-
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-
-
-
2
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
349678
Kiba
-
Flow-injection determination o ...
Bacillus cereus, Bacillus sp. (in: Bacteria)
Anal. Chim. Acta
218
161-166
1989
-
1
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2
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2
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1
2
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1
1
1
-
1
1
-
4
-
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1
-
4
-
-
-
-
2
-
-
-
-
-
-
-
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-
-
1
2
-
1
1
1
-
1
1
-
-
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-
-
-
-
-
349699
Kiba
High-performance liquid chroma ...
Bacillus subtilis
J. Chromatogr.
497
236-242
1989
-
1
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-
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-
1
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2
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1
-
2
-
-
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-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
349663
Livesey
Isolation and characterization ...
Bacillus subtilis
Methods Enzymol.
166
282-288
1988
-
-
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-
-
-
3
9
-
-
-
-
-
2
-
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1
-
-
-
1
-
6
-
-
-
-
-
2
-
-
2
1
-
-
-
-
-
2
-
-
-
-
3
1
9
-
-
-
-
-
-
-
1
-
-
1
-
6
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
349669
Nagata
Gene cloning and sequence dete ...
Geobacillus stearothermophilus
Biochemistry
27
9056-9062
1988
-
-
1
-
-
-
-
-
-
-
1
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-
3
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1
-
-
-
1
-
1
1
-
-
-
-
-
-
-
2
-
-
-
-
-
1
2
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
1
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
349697
Livesey
Determination of branched-chai ...
Bacillus subtilis, Lysinibacillus sphaericus
Methods Enzymol.
166
3-10
1988
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2
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-
-
-
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2
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-
-
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6
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-
4
-
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2
-
4
-
-
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-
-
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
349680
Shimoi
-
Leucine dehydrogenase of a the ...
Moorella thermoacetica
Agric. Biol. Chem.
51
3375-3381
1987
-
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1
-
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11
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3
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1
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1
-
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1
1
7
1
-
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3
-
1
-
1
3
-
-
-
-
-
1
3
-
-
-
-
-
-
11
-
-
3
-
-
-
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1
-
-
1
1
7
1
-
-
3
-
1
-
1
-
-
-
-
-
-
-
349554
Ohshima
-
Screening of thermostable leuc ...
Bacillus sp. (in: Bacteria), Lysinibacillus sphaericus
Biotechnol. Lett.
7
871-876
1985
-
-
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2
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2
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4
-
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4
-
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-
4
-
-
-
-
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-
-
-
-
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-
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-
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-
-
-
-
-
2
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
349661
Schuette
-
L-Leucine dehydrogenase from B ...
Bacillus cereus
Appl. Microbiol. Biotechnol.
22
306-317
1985
-
-
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1
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3
16
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2
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1
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1
-
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-
1
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7
1
-
-
-
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2
-
1
3
-
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-
-
-
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3
1
-
-
-
3
-
16
-
-
2
-
-
-
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1
-
-
1
-
7
1
-
-
-
-
2
-
1
-
-
-
-
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349662
Ohshima
-
Purification and characterizat ...
Geobacillus stearothermophilus
Arch. Microbiol.
141
407-411
1985
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-
5
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2
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1
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1
-
-
-
1
1
4
1
1
1
2
-
3
-
1
3
-
-
-
-
-
-
3
-
-
-
-
5
-
-
-
-
2
-
-
-
-
1
-
-
1
1
4
1
1
1
2
-
3
-
1
-
-
-
-
-
-
-
349673
Ohshima
-
Modification of leucine dehydr ...
Lysinibacillus sphaericus
Agric. Biol. Chem.
48
349-354
1984
-
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-
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1
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2
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-
-
-
-
2
-
-
2
-
-
-
-
-
-
2
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
349668
Hummel
-
Leucine dehydrogenase from Bac ...
Lysinibacillus sphaericus
Eur. J. Appl. Microbiol. Biotechnol.
12
22-27
1981
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
1
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
349679
Warth
Heat stability of Bacillus cer ...
Bacillus cereus
J. Bacteriol.
143
27-34
1980
-
-
-
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2
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-
2
-
-
1
-
-
-
2
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-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
1
-
-
-
2
-
-
-
-
-
-
-
-
-
-
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349671
Obermeier
-
Experiments on the role of leu ...
Bacillus subtilis, Bacillus subtilis 168M
FEMS Microbiol. Lett.
5
81-83
1979
-
-
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2
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-
2
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4
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-
-
-
-
-
2
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-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
2
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
349665
Ohshima
Properties of crystalline leuc ...
Bacillus cereus, Bacillus megaterium, Bacillus pumilus, Bacillus subtilis, Brevibacillus brevis, Lysinibacillus sphaericus
J. Biol. Chem.
253
5719-5725
1978
-
-
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1
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16
14
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2
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7
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1
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1
-
28
1
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-
-
-
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-
12
6
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12
1
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-
16
6
14
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-
2
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-
-
-
1
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-
1
-
28
1
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-
349667
Ohshima
-
Leucine dehydrogenase of bacil ...
Lysinibacillus sphaericus
Agric. Biol. Chem.
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1739-1743
1978
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2
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1
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1
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2
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2
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2
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-
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-
1
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349666
Obermeier
Some physiological functions o ...
Bacillus subtilis
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59-63
1976
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2
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2
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2
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2
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1
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2
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349672
Oki
Antitumor activities of bacter ...
Lysinibacillus sphaericus
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33
286-288
1973
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1
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2
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2
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2
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2
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349675
Soda
Crystalline L-leucine dehydrog ...
Lysinibacillus sphaericus
Biochem. Biophys. Res. Commun.
44
931-935
1971
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2
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1
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1
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1
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5
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3
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3
1
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2
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1
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1
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5
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349658
Zink
-
L-Leucine dehydrogenase (Bacil ...
Bacillus cereus
Methods Enzymol.
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799-802
1970
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2
7
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1
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1
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1
2
4
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1
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2
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2
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2
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7
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1
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1
2
4
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1
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349660
Zink
-
The distribution and substrate ...
Bacillus cereus, Bacillus megaterium, Bacillus mycoides, Bacillus niger, Bacillus pumilus, Bacillus subtilis, Lysinibacillus sphaericus
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72-77
1962
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14
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7
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1
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4
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19
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1
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14
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14
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14
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1
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4
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19
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1
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349659
Sanwal
L-Leucine dehydrogenase of Bac ...
Bacillus cereus
Arch. Biochem. Biophys.
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1961
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5
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2
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1
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1
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2
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1
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3
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3
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2
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5
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1
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1
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2
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1
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