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Literature summary for 1.4.1.9 extracted from

  • Sanwal, B.D.; Zink, M.W.
    L-Leucine dehydrogenase of Bacillus cereus (1961), Arch. Biochem. Biophys., 94, 430-435.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
PCMB reversed by L-Cys Bacillus cereus
Sulfide Na2S Bacillus cereus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.1
-
NADH
-
Bacillus cereus
0.16
-
NAD+
-
Bacillus cereus
2.2
-
3-methyl-2-oxobutanoate
-
Bacillus cereus
6.2
-
L-Leu
-
Bacillus cereus
13
-
NH4+
-
Bacillus cereus

Organism

Organism UniProt Comment Textmining
Bacillus cereus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Bacillus cereus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Bacillus cereus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-Leu + H2O + NAD+ the equilibrium favors synthesis of L-Leu Bacillus cereus 4-methyl-2-oxopentanoate + NH3 + NADH
-
r
L-Val + H2O + NAD+
-
Bacillus cereus 3-methyl-2-oxobutanoate + NH3 + NADH
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
11.3
-
oxidative deamination Bacillus cereus

Cofactor

Cofactor Comment Organism Structure
additional information no activity with NADPH Bacillus cereus
additional information no activity with NADP+ Bacillus cereus
NAD+
-
Bacillus cereus
NADH
-
Bacillus cereus