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Literature summary for 1.4.1.9 extracted from

  • Zink, M.W.; Sanwal, B.D.
    L-Leucine dehydrogenase (Bacillus cereus) (1970), Methods Enzymol., 17A, 799-802.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
PCMB
-
Bacillus cereus
Sulfide
-
Bacillus cereus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.12
-
NADH
-
Bacillus cereus
0.16
-
NAD+
-
Bacillus cereus
2
-
L-Val
-
Bacillus cereus
2.2
-
3-methyl-2-oxobutanoate
-
Bacillus cereus
5.2
-
L-Ile
-
Bacillus cereus
6.2
-
L-Leu
-
Bacillus cereus
13
-
NH4+
-
Bacillus cereus

Organism

Organism UniProt Comment Textmining
Bacillus cereus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Bacillus cereus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Bacillus cereus

Storage Stability

Storage Stability Organism
-20°C, 50% glycerol, stable for 1 year Bacillus cereus
-20°C, stable for over 1 month Bacillus cereus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-2-aminobutanoate + H2O + NAD+
-
Bacillus cereus 2-oxobutanoate + NH3 + NADH + H+
-
?
L-Ile + H2O + NAD+
-
Bacillus cereus 3-methyl-2-oxopentanoate + NH3 + NADH
-
r
L-Leu + H2O + NAD+
-
Bacillus cereus 4-methyl-2-oxopentanoate + NH3 + NADH
-
r
L-Val + H2O + NAD+
-
Bacillus cereus 3-methyl-2-oxobutanoate + NH3 + NADH
-
r

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
11.3
-
oxidative deamination Bacillus cereus

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Bacillus cereus
NADH
-
Bacillus cereus