Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-oxoisovalerate | 65 mM, 50% loss of specific activity | Priestia megaterium | |
CoCl2 | - |
Priestia megaterium | |
Cu2+ | CuSO4: less inhibition compared with HgCl2 and CoCl2 | Priestia megaterium | |
Hg2+ | - |
Priestia megaterium | |
L-valine | 10 mM, about 50% loss of specific activity | Priestia megaterium | |
Mn2+ | MnCl2: less inhibition compared with HgCl2 and CoCl2 | Priestia megaterium |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.05 | - |
NADH | - |
Priestia megaterium | |
1.5 | - |
2-oxoisovalerate | - |
Priestia megaterium | |
153 | - |
NH4+ | - |
Priestia megaterium |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Priestia megaterium | - |
strain ATCC 39118 | - |
Purification (Comment) | Organism |
---|---|
- |
Priestia megaterium |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
7.8 | - |
- |
Priestia megaterium |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-2-aminobutyrate + H2O + NAD+ | 9% of the activity with L-leucine | Priestia megaterium | 2-oxobutyrate + NADH + NH3 | reductive amination: 28.1% of the activity with 2-oxoisovalerate | ? | |
L-2-aminobutyrate + H2O + NAD+ | 9% of the activity with L-leucine | Priestia megaterium | 2-oxobutyrate + NADH + NH3 | reductive amination: 28.1% of the activity with 2-oxoisovalerate | r | |
L-2-aminobutyrate + H2O + NAD+ | 9% of the activity with L-leucine | Priestia megaterium | 2-oxobutyrate + NADH + NH3 | 2-oxobutyrate is identical with alpha-ketobutyrate | ? | |
L-2-aminobutyrate + H2O + NAD+ | 9% of the activity with L-leucine | Priestia megaterium | 2-oxobutyrate + NADH + NH3 | 2-oxobutyrate is identical with alpha-ketobutyrate | r | |
L-2-aminobutyrate + H2O + NAD+ | L-2-aminobutyrate is identical with L-alpha-aminobutyrate | Priestia megaterium | 2-oxobutyrate + NADH + NH3 | reductive amination: 28.1% of the activity with 2-oxoisovalerate | ? | |
L-2-aminobutyrate + H2O + NAD+ | L-2-aminobutyrate is identical with L-alpha-aminobutyrate | Priestia megaterium | 2-oxobutyrate + NADH + NH3 | reductive amination: 28.1% of the activity with 2-oxoisovalerate | r | |
L-2-aminobutyrate + H2O + NAD+ | L-2-aminobutyrate is identical with L-alpha-aminobutyrate | Priestia megaterium | 2-oxobutyrate + NADH + NH3 | 2-oxobutyrate is identical with alpha-ketobutyrate | ? | |
L-2-aminobutyrate + H2O + NAD+ | L-2-aminobutyrate is identical with L-alpha-aminobutyrate | Priestia megaterium | 2-oxobutyrate + NADH + NH3 | 2-oxobutyrate is identical with alpha-ketobutyrate | r | |
L-alanine + H2O + NAD+ | 3% of the activity with L-valine | Priestia megaterium | pyruvate + NH3 + NADH | reductive amination: 2% of the activity with 2-oxoisovalerate | r | |
L-alanine + H2O + NAD+ | 3% of the activity with L-valine | Priestia megaterium | pyruvate + NH3 + NADH | pyruvate is identical with alpha-ketopropanoate and 2-oxopropanoate | r | |
L-isoleucine + H2O + NAD+ | 61.3% of the activity with L-leucine | Priestia megaterium | 3-methyl-2-oxopentanoate + NADH + NH3 | 2-oxo-3-methylvalerate is identical with alpha-keto-beta-methylpentanoate | r | |
L-isoleucine + H2O + NAD+ | 61.3% of the activity with L-leucine | Priestia megaterium | 3-methyl-2-oxopentanoate + NADH + NH3 | reductive amination: 21.9% of the activity with 2-oxoisovalerate | r | |
L-leucine + H2O + NAD+ | preferred substrate | Priestia megaterium | 2-oxoisocaproate + NADH + NH3 | 2-oxoisocaproate is identical with 2-oxo-4-methylpentanoate, 2-oxoisohexanoate and alpha-ketoisocaproate | r | |
L-leucine + H2O + NAD+ | preferred substrate | Priestia megaterium | 2-oxoisocaproate + NADH + NH3 | reductive amination: 21.1% of the activity with 2-oxoisovalerate | r | |
L-norleucine + H2O + NAD+ | 4.7% of the activity with L-valine | Priestia megaterium | 2-oxocaproate + NADH + NH3 | reductive amination: 9.7% of the activity with 2-oxoisovalerate | r | |
L-norleucine + H2O + NAD+ | 4.7% of the activity with L-valine | Priestia megaterium | 2-oxocaproate + NADH + NH3 | 2-oxocaproate is identical with 2-oxohexanoate and alpha-keto-n-hexanoate | r | |
L-norvaline + H2O + NAD+ | 47.2% of the activity with L-leucine | Priestia megaterium | 2-oxovalerate + NH3 + NADH | reductive amination: 37% of the activity with 2-oxoisovalerate | r | |
L-norvaline + H2O + NAD+ | 47.2% of the activity with L-leucine | Priestia megaterium | 2-oxovalerate + NH3 + NADH | 2-oxovalerate is identical with 2-oxopentanoate and alpha-ketovalerate | r | |
L-valine + NAD(P)+ + H2O | NAD+ | Priestia megaterium | 2-oxoisovalerate + NH3 + NAD(P)H | NADH | r | |
L-valine + NAD(P)+ + H2O | NAD+ | Priestia megaterium | 2-oxoisovalerate + NH3 + NAD(P)H | 2-oxoisovalerate is identical with 3-methyl-2-oxobutanoate and alpha-ketoisovalerate | r | |
L-valine + NAD(P)+ + H2O | NAD+ | Priestia megaterium | 2-oxoisovalerate + NH3 + NAD(P)H | reductive amination: preferred substrate | r | |
L-valine + NAD(P)+ + H2O | 88.9% of the activity with L-leucine | Priestia megaterium | 2-oxoisovalerate + NH3 + NAD(P)H | NADH | r | |
L-valine + NAD(P)+ + H2O | 88.9% of the activity with L-leucine | Priestia megaterium | 2-oxoisovalerate + NH3 + NAD(P)H | 2-oxoisovalerate is identical with 3-methyl-2-oxobutanoate and alpha-ketoisovalerate | r | |
L-valine + NAD(P)+ + H2O | 88.9% of the activity with L-leucine | Priestia megaterium | 2-oxoisovalerate + NH3 + NAD(P)H | reductive amination: preferred substrate | r | |
additional information | no activity with the D-isomers of the substrates | Priestia megaterium | additional information | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
47 | - |
- |
Priestia megaterium |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
0.75 M ammonia buffer pH 8.5, half-life: 12 days | Priestia megaterium |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
reductive amination | Priestia megaterium |
10.5 | - |
oxidative deamination of L-valine | Priestia megaterium |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Priestia megaterium | |
NADH | NADH could not be replaced with NADPH for the reductive amination | Priestia megaterium |