Cloned (Comment) | Organism |
---|---|
gene gdhA, phylogenetic analysis, expression of His6-tagged GdhA in Escherichia coli, ectopic expression in the cytoplasm of transgenic Oryza sativa plants. The introduced gdhA, together with the endogenous glutamine synthetase, directly assimilates NH4 + absorbed from the roots. NADP(H)-GDH activities in the gdhA-transgenic lines are markedly higher than those in a control line | Aspergillus niger |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.041 | - |
NADP+ | pH 8.0, 30°C, recombinant enzyme | Aspergillus niger | |
0.34 | - |
NADPH | pH 8.0, 30°C, recombinant enzyme | Aspergillus niger | |
1.16 | - |
NH3 | pH 8.0, 30°C, recombinant enzyme | Aspergillus niger | |
3.97 | - |
2-oxoglutarate | pH 8.0, 30°C, recombinant enzyme | Aspergillus niger | |
15.15 | - |
L-glutamate | pH 8.0, 30°C, recombinant enzyme | Aspergillus niger |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | Ca2+ has no effects on the aminating activity of NADP(H)-GDH | Aspergillus niger |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamate + H2O + NADP+ | Aspergillus niger | - |
2-oxoglutarate + NH3 + NADPH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus niger | - |
gene gdhA | - |
Purification (Comment) | Organism |
---|---|
recombinant His6-tagged GdhA from Escherichia coli by nickel affinity chromatography | Aspergillus niger |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
root | - |
Aspergillus niger | - |
shoot | - |
Aspergillus niger | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamate + H2O + NADP+ | - |
Aspergillus niger | 2-oxoglutarate + NH3 + NADPH + H+ | - |
r |
Synonyms | Comment | Organism |
---|---|---|
GDH | - |
Aspergillus niger |
NADP(H)-dependent glutamate dehydrogenase | - |
Aspergillus niger |
NADP(H)-GDH | - |
Aspergillus niger |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Aspergillus niger |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Aspergillus niger |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Aspergillus niger | |
NADPH | - |
Aspergillus niger |
General Information | Comment | Organism |
---|---|---|
metabolism | both NADP(H)-GDH (gdhA) and glutamine synthetase play important roles in ammonium assimilation | Aspergillus niger |