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Literature summary for 1.4.1.4 extracted from

  • Abiko, T.; Wakayama, M.; Kawakami, A.; Obara, M.; Kisaka, H.; Miwa, T.; Aoki, N.; Ohsugi, R.
    Changes in nitrogen assimilation, metabolism, and growth in transgenic rice plants expressing a fungal NADP(H)-dependent glutamate dehydrogenase (gdhA) (2010), Planta, 232, 299-311.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene gdhA, phylogenetic analysis, expression of His6-tagged GdhA in Escherichia coli, ectopic expression in the cytoplasm of transgenic Oryza sativa plants. The introduced gdhA, together with the endogenous glutamine synthetase, directly assimilates NH4 + absorbed from the roots. NADP(H)-GDH activities in the gdhA-transgenic lines are markedly higher than those in a control line Aspergillus niger

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.041
-
NADP+ pH 8.0, 30°C, recombinant enzyme Aspergillus niger
0.34
-
NADPH pH 8.0, 30°C, recombinant enzyme Aspergillus niger
1.16
-
NH3 pH 8.0, 30°C, recombinant enzyme Aspergillus niger
3.97
-
2-oxoglutarate pH 8.0, 30°C, recombinant enzyme Aspergillus niger
15.15
-
L-glutamate pH 8.0, 30°C, recombinant enzyme Aspergillus niger

Metals/Ions

Metals/Ions Comment Organism Structure
additional information Ca2+ has no effects on the aminating activity of NADP(H)-GDH Aspergillus niger

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-glutamate + H2O + NADP+ Aspergillus niger
-
2-oxoglutarate + NH3 + NADPH + H+
-
r

Organism

Organism UniProt Comment Textmining
Aspergillus niger
-
gene gdhA
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His6-tagged GdhA from Escherichia coli by nickel affinity chromatography Aspergillus niger

Source Tissue

Source Tissue Comment Organism Textmining
root
-
Aspergillus niger
-
shoot
-
Aspergillus niger
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-glutamate + H2O + NADP+
-
Aspergillus niger 2-oxoglutarate + NH3 + NADPH + H+
-
r

Synonyms

Synonyms Comment Organism
GDH
-
Aspergillus niger
NADP(H)-dependent glutamate dehydrogenase
-
Aspergillus niger
NADP(H)-GDH
-
Aspergillus niger

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Aspergillus niger

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Aspergillus niger

Cofactor

Cofactor Comment Organism Structure
NADP+
-
Aspergillus niger
NADPH
-
Aspergillus niger

General Information

General Information Comment Organism
metabolism both NADP(H)-GDH (gdhA) and glutamine synthetase play important roles in ammonium assimilation Aspergillus niger