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Literature summary for 1.4.1.4 extracted from
- NADP-glutamate dehydrogenase activity in nonstarter lactic acid bacteria: effects of temperature, pH and NaCl on enzyme activity and expression (2010), J. Appl. Microbiol., 109, 1763-1774.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene gdh, quantitative real-time PCR expression analysis in different cheese cultures | Lactiplantibacillus plantarum |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | - |
Lactiplantibacillus plantarum | 5737 | - |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-glutamate + H2O + NADP+ | Lactiplantibacillus plantarum | - |
2-oxoglutarate + NH3 + NADPH + H+ | - |
r |  |
| L-glutamate + H2O + NADP+ | Lactiplantibacillus plantarum DPPMA49 | - |
2-oxoglutarate + NH3 + NADPH + H+ | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Lactiplantibacillus plantarum | Q2WCS9 | - |
- |
| Lactiplantibacillus plantarum DPPMA49 | Q2WCS9 | - |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| additional information | strain DPPMA49 shows the highest NADP-GDH activity under temperature, pH and NaCl values found during cheese ripening. Enzyme production conditions and activity profiles, overview | Lactiplantibacillus plantarum | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 5.64 | - |
strain DPPMA49, pH 8.0, 37°C, comparison to activity in diverse other DPPMA strains of Lactobacillus plantarum and to other Lactobacilli, overview | Lactiplantibacillus plantarum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-glutamate + H2O + NADP+ | - |
Lactiplantibacillus plantarum | 2-oxoglutarate + NH3 + NADPH + H+ | - |
r | |
| L-glutamate + H2O + NADP+ | - |
Lactiplantibacillus plantarum DPPMA49 | 2-oxoglutarate + NH3 + NADPH + H+ | - |
r | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| NADP-GDH | - |
Lactiplantibacillus plantarum |
| NADP-glutamate dehydrogenase | - |
Lactiplantibacillus plantarum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 13 | - |
strain DPPMA49 shows highest enzyme activity under condition 13°C, pH 5.5, NaCl 5.62% | Lactiplantibacillus plantarum |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
optimal enzyme production conditions and activity profile, overview | Lactiplantibacillus plantarum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
strain DPPMA49 shows highest enzyme activity under condition 13°C, pH 5.5, NaCl 5.62% | Lactiplantibacillus plantarum |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
optimal enzyme production conditions and activity profile, overview | Lactiplantibacillus plantarum |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADP+ | - |
Lactiplantibacillus plantarum | |
| NADPH | - |
Lactiplantibacillus plantarum | |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Lactiplantibacillus plantarum | GDH expression of strain DPPMA49 is downregulated by temperature below 13°C, expression analysis | down |
| Lactiplantibacillus plantarum | GDH expression of strain DPPMA49 is upregulated by NaCl at 1.87-5.62% | up |
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