KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.053 | - |
NADPH | reductive amination | Thermophilic bacillus | |
0.3 | - |
NADP+ | oxidative deamination | Thermophilic bacillus | |
1.1 | - |
L-glutamate | oxidative deamination | Thermophilic bacillus | |
1.3 | - |
2-oxoglutarate | reductive amination | Thermophilic bacillus | |
21 | - |
NH4+ | reductive amination | Thermophilic bacillus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamate + NADP+ + H2O | Thermophilic bacillus | - |
2-oxoglutarate + NADPH + NH3 | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermophilic bacillus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Thermophilic bacillus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
46.2 | - |
reductive amination at 55°C | Thermophilic bacillus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxoglutarate + NH4+ + NADPH | - |
Thermophilic bacillus | L-glutamate + NADP+ | - |
? | |
L-glutamate + NADP+ + H2O | - |
Thermophilic bacillus | 2-oxoglutarate + NADPH + NH3 | - |
r |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
after 300 min 60% activity | Thermophilic bacillus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
reductive amination | Thermophilic bacillus |
8.4 | - |
oxidative deamination | Thermophilic bacillus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4.7 | - |
inactivation | Thermophilic bacillus |
5.8 | 9 | highly thermostable | Thermophilic bacillus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Thermophilic bacillus | |
NADPH | - |
Thermophilic bacillus |