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Literature summary for 1.4.1.4 extracted from

  • Sarada, K.V.; Rao, N.A.; Venkitasubramanian, T.A.
    Isolation and characterisation of glutamate dehydrogenase from Mycobacterium smegmatis CDC 46 (1980), Biochim. Biophys. Acta, 615, 299-308.
    View publication on PubMed
Search for more literature for 1.4.1.4

Activating Compound

Activating Compound Comment Organism Structure
Zn2+ activates up to 0.1 mM Mycolicibacterium smegmatis

Inhibitors

Inhibitors Comment Organism Structure
4-chloromercuribenzoate at 1 mM and 10 mM, 30% inhibition and 100% inhibition Mycolicibacterium smegmatis
Hg2+ at 1 mM, 10% inhibition Mycolicibacterium smegmatis
Zn2+ over 0.1 mM Mycolicibacterium smegmatis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
 alpha6, 6 * 40000, SDS-PAGE Mycolicibacterium smegmatis
245500
-
 gel filtration Mycolicibacterium smegmatis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-glutamate + NADP+ + H2O Mycolicibacterium smegmatis
-
 2-oxoglutarate + NADPH + NH3
-
 r
L-glutamate + NADP+ + H2O Mycolicibacterium smegmatis CDC46
-
 2-oxoglutarate + NADPH + NH3
-
 r

Organism

Organism UniProt Comment Textmining
Mycolicibacterium smegmatis
-
-
-
Mycolicibacterium smegmatis CDC46
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Mycolicibacterium smegmatis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
80.2
-
 reductive amination Mycolicibacterium smegmatis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-glutamate + NADP+ + H2O
-
 Mycolicibacterium smegmatis 2-oxoglutarate + NADPH + NH3
-
 r
L-glutamate + NADP+ + H2O
-
 Mycolicibacterium smegmatis CDC46 2-oxoglutarate + NADPH + NH3
-
 r

Subunits

Subunits Comment Organism
homohexamer alpha6, 6 * 40000, SDS-PAGE Mycolicibacterium smegmatis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
 reductive amination and oxidative deamination Mycolicibacterium smegmatis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 reductive amination Mycolicibacterium smegmatis
8.8 9.8 oxidative deamination Mycolicibacterium smegmatis

Cofactor

Cofactor Comment Organism Structure
NADP+
-
 Mycolicibacterium smegmatis
NADPH
-
 Mycolicibacterium smegmatis