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Literature summary for 1.4.1.4 extracted from

  • Lebbink, J.H.G.; Eggen, R.I.L.; Geerling, A.C.M.; Consalvi, V.; Chiaraluce, R.; Scandurra, R.; de Vos, W.M.
    Exchange of domains of glutamate dehydrogenase from the hyperthermophilic archaeon Pyrococcus furiosus and the mesophilic bacterium Clostridium difficile: Effects on catalysis, thermoactivity and stability (1995), Protein Eng., 8, 1287-1294.
    View publication on PubMed
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Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli, hybrid proteins containing the Pyrococcus furiosus glutamate and the Clostridium difficile cofactor binding domain with reduced substrate binding affinity Pyrococcus furiosus
expression in Escherichia coli, hybrid proteins containing the Pyrococcus furiosus glutamate and the Clostridium difficile cofactor binding domain with reduced substrate binding affinity Clostridioides difficile

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-glutamate + NADP+ + H2O Pyrococcus furiosus
-
 2-oxoglutarate + NADPH + NH3
-
 r
L-glutamate + NADP+ + H2O Clostridioides difficile
-
 2-oxoglutarate + NADPH + NH3
-
 r

Organism

Organism UniProt Comment Textmining
Clostridioides difficile
-
-
-
Pyrococcus furiosus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-glutamate + NADP+ + H2O
-
 Pyrococcus furiosus 2-oxoglutarate + NADPH + NH3
-
 r
L-glutamate + NADP+ + H2O
-
 Clostridioides difficile 2-oxoglutarate + NADPH + NH3
-
 r

Cofactor

Cofactor Comment Organism Structure
NADP+
-
 Pyrococcus furiosus
NADP+
-
 Clostridioides difficile
NADPH
-
 Pyrococcus furiosus
NADPH
-
 Clostridioides difficile