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Literature summary for 1.4.1.4 extracted from

  • Aalen, N.; Steen, I.H.; Birkeland, N.K.; Lien, T.
    Purification and properties of an extremely thermostable NADP+-specific glutamate dehydrogenase from Archaeoglobus fulgidus (1997), Arch. Microbiol., 168, 536-539.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
KCl at 0.2 M three to fourfold stimulation of activity Archaeoglobus fulgidus
NaCl at 0.2 M three to fourfold stimulation of activity Archaeoglobus fulgidus

General Stability

General Stability Organism
K2HPO4 enhances the thermostability optimally at 1 mM concentration Archaeoglobus fulgidus

Inhibitors

Inhibitors Comment Organism Structure
O2 inactivation Archaeoglobus fulgidus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.02
-
NADPH reductive amination at 60°C Archaeoglobus fulgidus
0.06
-
NADP+ oxidative deamination at 60°C Archaeoglobus fulgidus
0.5
-
2-oxoglutarate reductive amination at 60°C Archaeoglobus fulgidus
3.9
-
L-glutamate oxidative deamination at 60°C Archaeoglobus fulgidus
4
-
NH3 reductive amination at 60°C Archaeoglobus fulgidus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
263000
-
gel filtration Archaeoglobus fulgidus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-glutamate + NADP+ + H2O Archaeoglobus fulgidus
-
2-oxoglutarate + NADPH + NH3
-
r
L-glutamate + NADP+ + H2O Archaeoglobus fulgidus 7324
-
2-oxoglutarate + NADPH + NH3
-
r

Organism

Organism UniProt Comment Textmining
Archaeoglobus fulgidus
-
-
-
Archaeoglobus fulgidus 7324
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Archaeoglobus fulgidus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-oxoglutarate + NH3 + NADH + H+ NH4Cl used in enzyme assay Archaeoglobus fulgidus L-glutamate + H2O + NAD+
-
r
2-oxoglutarate + NH3 + NADH + H+ NH4Cl used in enzyme assay Archaeoglobus fulgidus 7324 L-glutamate + H2O + NAD+
-
r
L-glutamate + NADP+ + H2O
-
Archaeoglobus fulgidus 2-oxoglutarate + NADPH + NH3
-
r
L-glutamate + NADP+ + H2O
-
Archaeoglobus fulgidus 7324 2-oxoglutarate + NADPH + NH3
-
r

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
95
-
reductive amination Archaeoglobus fulgidus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
100
-
50% activity after 140 min Archaeoglobus fulgidus
102
-
50% activity after 55 min Archaeoglobus fulgidus
105
-
50% activity after 20 min Archaeoglobus fulgidus
107
-
50% activity after 10 min Archaeoglobus fulgidus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
oxidative deamination at 60°C Archaeoglobus fulgidus
8.4
-
reductive amination at 60°C Archaeoglobus fulgidus

Cofactor

Cofactor Comment Organism Structure
NADP+
-
Archaeoglobus fulgidus
NADPH
-
Archaeoglobus fulgidus