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Literature summary for 1.4.1.4 extracted from
- Glutamate dehydrogenases from hyperthermophiles (2001), Methods Enzymol., 331, 26-41.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli BL21 | Thermococcus litoralis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00056 | - |
NH3 | recombinant enzyme, reductive amination | Thermococcus litoralis |  |
| 0.00065 | - |
NH3 | wild-type enzyme, reductive amination | Thermococcus litoralis | |
| 0.0098 | - |
NADP+ | recombinant enzyme, oxidative deamination | Thermococcus litoralis | |
| 0.0102 | - |
NADP+ | wild-type enzyme, oxidative deamination | Thermococcus litoralis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 45000 | - |
alpha6, 6 * 45000, SDS-PAGE, expressed in Escherichia coli | Thermococcus litoralis |
| 47000 | - |
x * 47000, gel filtration | Thermococcus litoralis |
| 47169 | - |
x * 47169, amino acid analysis | Thermococcus litoralis |
| 270000 | - |
gel filtration, expressed in Escherichia coli | Thermococcus litoralis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-glutamate + NADP+ + H2O | Thermococcus litoralis | - |
2-oxoglutarate + NADPH + NH3 | - |
r | |
| additional information | Thermococcus litoralis | glutamate dehydrogenase represents an enzymatic link between major catabolic and biosynthetic pathways via the tricarboxylic acid cycle intermediate 2-oxoglutarate | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermococcus litoralis | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Thermococcus litoralis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 194.3 | - |
oxidative deamination at 75°C | Thermococcus litoralis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-glutamate + NADP+ + H2O | - |
Thermococcus litoralis | 2-oxoglutarate + NADPH + NH3 | - |
r | |
| additional information | glutamate dehydrogenase represents an enzymatic link between major catabolic and biosynthetic pathways via the tricarboxylic acid cycle intermediate 2-oxoglutarate | Thermococcus litoralis | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 47000, gel filtration | Thermococcus litoralis |
| ? | x * 47169, amino acid analysis | Thermococcus litoralis |
| homohexamer | alpha6, 6 * 45000, SDS-PAGE, expressed in Escherichia coli | Thermococcus litoralis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 85 | - |
wild-type enzyme retains 100% activity after 3 h of incubation | Thermococcus litoralis |
| 85 | - |
recombinant enzyme retains 90% activity after 3 h of incubation | Thermococcus litoralis |
| 98 | - |
recombinant enzyme retains 50% activity after 1.5 h of incubation | Thermococcus litoralis |
| 98 | - |
wild-type enzyme retains 50% activity after 6.4 h of incubation | Thermococcus litoralis |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADP+ | - |
Thermococcus litoralis | |
| NADPH | - |
Thermococcus litoralis | |
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Release 2023.1 (January 2023)
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