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Literature summary for 1.4.1.3 extracted from

  • Marchi, L.; Polverini, E.; Degola, F.; Baruffini, E.; Restivo, F.M.
    Glutamate dehydrogenase isoenzyme 3 (GDH3) of Arabidopsis thaliana is less thermostable than GDH1 and GDH2 isoenzymes (2014), Plant Physiol. Biochem., 83, 225-231 .
    View publication on PubMed
Search for more literature for 1.4.1.3

Cloned(Commentary)

Cloned (Comment) Organism
expression in Saccharomyces cerevisiae Arabidopsis thaliana

Crystallization (Commentary)

Crystallization (Comment) Organism
homology modeling of the hexameric structure of the GDH3 isoenzyme, it is highly symmetric and is formed by the assembly of two equivalent trimers with a three-fold symmetry Arabidopsis thaliana

Protein Variants

Protein Variants Comment Organism
I397V/I406L/S411A mutation to residues found in isoforms Gdh1 and Gdh2, increase in thermal stability Arabidopsis thaliana
I406L/S411A mutation to residues found in isoforms Gdh1 and Gdh2, increase in thermal stability Arabidopsis thaliana
S411A mutation to residue found in isoforms Gdh1 and Gdh2, increase in thermal stability Arabidopsis thaliana

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q38946
-
-
Arabidopsis thaliana Q43314
-
-
Arabidopsis thaliana Q9S7A0
-
-

Source Tissue

Source Tissue Comment Organism Textmining
root
-
 Arabidopsis thaliana
-

Subunits

Subunits Comment Organism
More gene products genes Gdh1, Gdh2 and Gdh3 are three different Gdh subunits that randomly associate to form a complex array of homo- and hetero-hexamers Arabidopsis thaliana

Synonyms

Synonyms Comment Organism
GDH1
-
 Arabidopsis thaliana
GDH2
-
 Arabidopsis thaliana
GDH3
-
 Arabidopsis thaliana

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
 20 min, the activity of the Gdh1/Gdh3 heterohexamers is completely inactivated at 70°C Arabidopsis thaliana
70
-
 20 min, the activity of the Gdh3 homohexamer and the Gdh1/Gdh3 heterohexamers is completely inactivated at 70°C Arabidopsis thaliana
75
-
 20 min, the Gdh1homohexamer and the Gdh2/Gdh1 heterohexamers remain active Arabidopsis thaliana
75
-
 20 min, the Gdh2 homohexamer and the Gdh2/Gdh1 heterohexamers remain active Arabidopsis thaliana