KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1 | - |
NADP+ | pH 8.0, 70°C | uncultured bacterium | |
0.3 | - |
L-glutamate | pH 8.0, 70°C | uncultured bacterium | |
0.4 | - |
NAD+ | pH 8.0, 70°C | uncultured bacterium |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40500 | - |
6 * 40500, SDS-PAGE | uncultured bacterium |
250500 | - |
gel filtration | uncultured bacterium |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamate + H2O + NAD+ | uncultured bacterium | - |
2-oxoglutarate + NH3 + NADH + H+ | - |
ir | |
L-glutamate + H2O + NADP+ | uncultured bacterium | - |
2-oxoglutarate + NH3 + NADPH + H+ | - |
ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
uncultured bacterium | - |
GWE1, a Gram-positive microaerophilic microorganism isolated from from the interior of a sterilization drying oven | - |
Purification (Comment) | Organism |
---|---|
native enzyme 22.7fold by anion exchange chromatography and gel filtration to homogeneity | uncultured bacterium |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
79.4 | - |
purified enzyme, pH 8.0, 70°C | uncultured bacterium |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamate + H2O + NAD+ | - |
uncultured bacterium | 2-oxoglutarate + NH3 + NADH + H+ | - |
ir | |
L-glutamate + H2O + NADP+ | - |
uncultured bacterium | 2-oxoglutarate + NH3 + NADPH + H+ | - |
ir | |
additional information | no activity in the amination reaction direction with NADH or NADPH, 2-oxoglutarate and ammonia | uncultured bacterium | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
hexamer | 6 * 40500, SDS-PAGE | uncultured bacterium |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
glutamate oxidation | uncultured bacterium |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | 100 | activity range, profile overview. Instability of the coenzyme at temperatures above 95°C | uncultured bacterium |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
100 | - |
purified enzyme, 70% activity remaining after over 5 h | uncultured bacterium |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
glutamate oxidation | uncultured bacterium |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.9 | 9 | activity range, profile overview | uncultured bacterium |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | the enzyme can use both NAD+ and NADP+ as electron acceptors, displaying more affinity for NADP+ than for NAD+. No activity with NADH or NADPH | uncultured bacterium | |
NAD+ | - |
uncultured bacterium | |
NADP+ | - |
uncultured bacterium |