Application | Comment | Organism |
---|---|---|
additional information | C323 plays an important role in catalysis by human GDH isozymes | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
mutant proteins expressed in Escherichia coli as soluble proteins | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
C323G | decreased turnover rate of both isoenzymes as compared to wild-type | Homo sapiens |
C323L | decreased turnover rate of both isoenzymes as compared to wild-type | Homo sapiens |
C323M | decreased turnover rate of both isoenzymes as compared to wild-type | Homo sapiens |
C323R | decreased turnover rate of both isoenzymes as compared to wild-type | Homo sapiens |
C323Y | decreased turnover rate of both isoenzymes as compared to wild-type | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
p-chloromercuribenzoic acid | progressive decrease in enzyme activity of both isoenzymes, inhibition is not affected by addition of GTP or ADP | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.081 | - |
NADH | hGDH1, wild-type | Homo sapiens | |
0.086 | - |
NADH | hGDH2, wild-type | Homo sapiens | |
0.117 | - |
NADH | hGDH1, mutant C323L | Homo sapiens | |
0.119 | - |
NADH | hGDH1, mutant C323G | Homo sapiens | |
0.119 | - |
NADH | hGDH1, mutant C323M | Homo sapiens | |
0.119 | - |
NADH | hGDH2, mutant C323M | Homo sapiens | |
0.121 | - |
NADH | hGDH2, mutant C323L | Homo sapiens | |
0.122 | - |
NADH | hGDH1, mutant C323R | Homo sapiens | |
0.125 | - |
NADH | hGDH2, mutant C323R | Homo sapiens | |
0.128 | - |
NADH | hGDH2, mutant C323G | Homo sapiens | |
0.129 | - |
NADH | hGDH1, mutant C323Y | Homo sapiens | |
0.138 | - |
NADH | hGDH2, mutant C323Y | Homo sapiens | |
1.25 | - |
2-oxoglutarate | hGDH1, wild-type | Homo sapiens | |
1.34 | - |
2-oxoglutarate | hGDH1, mutant C323L | Homo sapiens | |
1.36 | - |
2-oxoglutarate | hGDH1, mutant C323Y | Homo sapiens | |
1.38 | - |
2-oxoglutarate | hGDH1, mutant C323M | Homo sapiens | |
1.39 | - |
2-oxoglutarate | hGDH1, mutant C323R | Homo sapiens | |
1.39 | - |
2-oxoglutarate | hGDH2, wild-type | Homo sapiens | |
1.4 | - |
2-oxoglutarate | hGDH1, mutant C323G | Homo sapiens | |
1.41 | - |
2-oxoglutarate | hGDH2, mutant C323Y | Homo sapiens | |
1.45 | - |
2-oxoglutarate | hGDH2, mutant C323L | Homo sapiens | |
1.45 | - |
2-oxoglutarate | hGDH2, mutant C323M | Homo sapiens | |
1.5 | - |
2-oxoglutarate | hGDH2, mutant C323R | Homo sapiens | |
1.5 | 2 | 2-oxoglutarate | hGDH2, mutant C323G | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
to homogeneity | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxoglutarate + NH3 + NADH | - |
Homo sapiens | L-glutamate + H2O + NAD+ | - |
r |
Synonyms | Comment | Organism |
---|---|---|
glutamate dehydrogenase | - |
Homo sapiens |
hGDH1 | - |
Homo sapiens |
hGDH2 | - |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
14 | - |
2-oxoglutarate | hGDH1, mutant C323Y | Homo sapiens | |
15 | - |
2-oxoglutarate | hGDH1, mutant C323R | Homo sapiens | |
17 | - |
2-oxoglutarate | hGDH1, mutant C323G | Homo sapiens | |
17 | - |
2-oxoglutarate | hGDH1, mutant C323L | Homo sapiens | |
17 | - |
2-oxoglutarate | hGDH2, mutant C323Y | Homo sapiens | |
19 | - |
2-oxoglutarate | hGDH1, mutant C323M | Homo sapiens | |
19 | - |
2-oxoglutarate | hGDH2, mutant C323M | Homo sapiens | |
19 | - |
2-oxoglutarate | hGDH2, mutant C323R | Homo sapiens | |
20 | - |
2-oxoglutarate | hGDH2, mutant C323L | Homo sapiens | |
21 | - |
2-oxoglutarate | hGDH2, mutant C323G | Homo sapiens | |
104 | - |
2-oxoglutarate | hGDH1, wild-type | Homo sapiens | |
130 | - |
2-oxoglutarate | hGDH2, wild-type | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Homo sapiens |