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Literature summary for 1.4.1.3 extracted from

  • Yang, S.J.; Cho, E.H.; Choi, M.M.; Lee, H.J.; Huh, J.W.; Choi, S.Y.; Cho, S.W.
    Critical role of the cysteine 323 residue in the catalytic activity of human glutamate dehydrogenase isozymes (2005), Mol. Cell, 19, 97-103.
    View publication on PubMed

Application

Application Comment Organism
additional information C323 plays an important role in catalysis by human GDH isozymes Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
mutant proteins expressed in Escherichia coli as soluble proteins Homo sapiens

Protein Variants

Protein Variants Comment Organism
C323G decreased turnover rate of both isoenzymes as compared to wild-type Homo sapiens
C323L decreased turnover rate of both isoenzymes as compared to wild-type Homo sapiens
C323M decreased turnover rate of both isoenzymes as compared to wild-type Homo sapiens
C323R decreased turnover rate of both isoenzymes as compared to wild-type Homo sapiens
C323Y decreased turnover rate of both isoenzymes as compared to wild-type Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
p-chloromercuribenzoic acid progressive decrease in enzyme activity of both isoenzymes, inhibition is not affected by addition of GTP or ADP Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.081
-
NADH hGDH1, wild-type Homo sapiens
0.086
-
NADH hGDH2, wild-type Homo sapiens
0.117
-
NADH hGDH1, mutant C323L Homo sapiens
0.119
-
NADH hGDH1, mutant C323G Homo sapiens
0.119
-
NADH hGDH1, mutant C323M Homo sapiens
0.119
-
NADH hGDH2, mutant C323M Homo sapiens
0.121
-
NADH hGDH2, mutant C323L Homo sapiens
0.122
-
NADH hGDH1, mutant C323R Homo sapiens
0.125
-
NADH hGDH2, mutant C323R Homo sapiens
0.128
-
NADH hGDH2, mutant C323G Homo sapiens
0.129
-
NADH hGDH1, mutant C323Y Homo sapiens
0.138
-
NADH hGDH2, mutant C323Y Homo sapiens
1.25
-
2-oxoglutarate hGDH1, wild-type Homo sapiens
1.34
-
2-oxoglutarate hGDH1, mutant C323L Homo sapiens
1.36
-
2-oxoglutarate hGDH1, mutant C323Y Homo sapiens
1.38
-
2-oxoglutarate hGDH1, mutant C323M Homo sapiens
1.39
-
2-oxoglutarate hGDH1, mutant C323R Homo sapiens
1.39
-
2-oxoglutarate hGDH2, wild-type Homo sapiens
1.4
-
2-oxoglutarate hGDH1, mutant C323G Homo sapiens
1.41
-
2-oxoglutarate hGDH2, mutant C323Y Homo sapiens
1.45
-
2-oxoglutarate hGDH2, mutant C323L Homo sapiens
1.45
-
2-oxoglutarate hGDH2, mutant C323M Homo sapiens
1.5
-
2-oxoglutarate hGDH2, mutant C323R Homo sapiens
1.5 2 2-oxoglutarate hGDH2, mutant C323G Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
to homogeneity Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-oxoglutarate + NH3 + NADH
-
Homo sapiens L-glutamate + H2O + NAD+
-
r

Synonyms

Synonyms Comment Organism
glutamate dehydrogenase
-
Homo sapiens
hGDH1
-
Homo sapiens
hGDH2
-
Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
14
-
2-oxoglutarate hGDH1, mutant C323Y Homo sapiens
15
-
2-oxoglutarate hGDH1, mutant C323R Homo sapiens
17
-
2-oxoglutarate hGDH1, mutant C323G Homo sapiens
17
-
2-oxoglutarate hGDH1, mutant C323L Homo sapiens
17
-
2-oxoglutarate hGDH2, mutant C323Y Homo sapiens
19
-
2-oxoglutarate hGDH1, mutant C323M Homo sapiens
19
-
2-oxoglutarate hGDH2, mutant C323M Homo sapiens
19
-
2-oxoglutarate hGDH2, mutant C323R Homo sapiens
20
-
2-oxoglutarate hGDH2, mutant C323L Homo sapiens
21
-
2-oxoglutarate hGDH2, mutant C323G Homo sapiens
104
-
2-oxoglutarate hGDH1, wild-type Homo sapiens
130
-
2-oxoglutarate hGDH2, wild-type Homo sapiens

Cofactor

Cofactor Comment Organism Structure
NADH
-
Homo sapiens