Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Pyrococcus furiosus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
46000 | - |
6 * 46000, recombinant enzyme, SDS-PAGE | Pyrococcus furiosus |
270000 | - |
recombinant enzyme, only hexameric form is enzymatically active, gel filtration | Pyrococcus furiosus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus furiosus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native and recombinant enzyme | Pyrococcus furiosus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
411 | - |
native enzyme at 100°C | Pyrococcus furiosus |
419 | - |
recombinant enzyme at 75°C | Pyrococcus furiosus |
423 | - |
recombinant enzyme at 90°C | Pyrococcus furiosus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamate + H2O + NAD(P)+ | - |
Pyrococcus furiosus | 2-oxoglutarate + NH3 + NAD(P)H | - |
r |
Subunits | Comment | Organism |
---|---|---|
hexamer | 6 * 46000, recombinant enzyme, SDS-PAGE | Pyrococcus furiosus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
85 | - |
native and recombinant enzyme | Pyrococcus furiosus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 90 | - |
Pyrococcus furiosus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
100 | - |
native enzyme, half-life at 100°C: 10.5 h, recombinant enzyme, half-life at 75°C: 7h, at 90°C: 8.1 h | Pyrococcus furiosus |