General Stability | Organism |
---|---|
glycerol, 20% v/v, protects against cold inactivation | Azospirillum brasilense |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
alanine | weak inhibition at pH 8.5, strong inhibition at pH 10.0 | Azospirillum brasilense | |
glutamate | weak inhibition at pH 8.5, strong inhibition at pH 10.0 | Azospirillum brasilense | |
histidine | weak inhibition at pH 8.5, strong inhibition at pH 10.0 | Azospirillum brasilense | |
lysine | weak inhibition at pH 8.5, strong inhibition at pH 10.0 | Azospirillum brasilense |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.004 | - |
NADP+ | NADP+-dependent activity | Azospirillum brasilense | |
0.013 | - |
NADPH | NADPH-dependent activity | Azospirillum brasilense | |
0.25 | - |
2-oxoglutarate | NADPH-dependent activity | Azospirillum brasilense | |
0.38 | - |
NH3 | biphasic Lineweaver-Burk plot suggests 2 Km values | Azospirillum brasilense | |
0.5 | - |
NADH | NADH-dependent activity | Azospirillum brasilense | |
5 | - |
2-oxoglutarate | NADH-dependent activity | Azospirillum brasilense | |
10 | - |
L-glutamate | NADP+-dependent activity | Azospirillum brasilense | |
66 | - |
NH3 | NADH-dependent activity | Azospirillum brasilense | |
100 | - |
NH3 | biphasic Lineweaver-Burk plot suggests 2 Km values | Azospirillum brasilense |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
48000 | - |
6 * 48000, SDS-PAGE | Azospirillum brasilense |
285000 | - |
gel filtration | Azospirillum brasilense |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Azospirillum brasilense | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Azospirillum brasilense |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
41.9 | - |
- |
Azospirillum brasilense |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxoglutarate + NH3 + NADH + H+ | - |
Azospirillum brasilense | L-glutamate + H2O + NAD+ | - |
r | |
2-oxoglutarate + NH3 + NADH + H+ | NH4Cl used in enzyme assay | Azospirillum brasilense | L-glutamate + H2O + NAD+ | - |
r | |
2-oxoglutarate + NH3 + NADPH + H+ | NH4Cl used in enzyme assay | Azospirillum brasilense | L-glutamate + H2O + NADP+ | - |
r | |
L-glutamate + H2O + NAD(P)+ | highly specific for 2-oxoglutarate and glutamate | Azospirillum brasilense | 2-oxoglutarate + NH3 + NAD(P)H | - |
r |
Subunits | Comment | Organism |
---|---|---|
hexamer | 6 * 48000, SDS-PAGE | Azospirillum brasilense |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
- |
- |
complete loss of activity after 5 h, 20% glycerol protects from inactivation | Azospirillum brasilense |
5 | - |
moderately stable above | Azospirillum brasilense |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.33 | - |
L-glutamate | cofactor NAD+, value below 200.0 | Azospirillum brasilense | |
61 | - |
glutamate | cofactor NADP+ | Azospirillum brasilense | |
183 | - |
2-oxoglutarate | cofactor NADH | Azospirillum brasilense | |
200 | - |
NADPH | NADPH-dependent activity | Azospirillum brasilense |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.4 | 10 | NADPH + 2-oxoglutarate + NH3, potassium phosphate buffer | Azospirillum brasilense |
8.5 | 8.6 | NADPH + NH3 + 2-oxoglutarate, Tris-HCl buffer | Azospirillum brasilense |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7 | 9 | room temperature, 20% v/v glycerol, stable, room temperature, 1% v/v glycerol, 25% loss of activity after 48 h | Azospirillum brasilense |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
1.1 | - |
glutamate | at pH 10.0 | Azospirillum brasilense | |
3.7 | - |
lysine | at pH 10.0 | Azospirillum brasilense | |
4.1 | - |
glutamate | at pH 8.5 | Azospirillum brasilense | |
6.1 | - |
histidine | at pH 10.0 | Azospirillum brasilense | |
6.2 | - |
alanine | at pH 10.0 | Azospirillum brasilense | |
9.3 | - |
lysine | at pH 8.5 | Azospirillum brasilense | |
23.2 | - |
alanine | at pH 8.5 | Azospirillum brasilense |