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Literature summary for 1.4.1.23 extracted from

  • Nguyen, L.T.; Nguyen, K.T.; Kopecky, J.; Nova, P.; Novotna, J.; Behal, V.
    Purification and characterization of a novel valine dehydrogenase from Streptomyces aureofaciens (1995), Biochim. Biophys. Acta, 1251, 186-190.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
4-hydroxymercuribenzoate 0.01 mM, 100% inhibition Kitasatospora aureofaciens
iodoacetamide 1 mM, 50% inhibition Kitasatospora aureofaciens
Mn2+ 1 mM, 60% inhibition Kitasatospora aureofaciens
additional information activity is not significantly affected by 1 mM ATP, ADP, AMP, Mg2+, Ca2+, Cu2+, Zn2+, and metabolites of the citric acid cycle Kitasatospora aureofaciens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.42
-
2-Oxoisohexanoate pH 10.4, temperature not specified in the publication Kitasatospora aureofaciens
0.46
-
2-oxoisovalerate pH 10.4, temperature not specified in the publication Kitasatospora aureofaciens
0.75
-
L-valine pH 10.4, temperature not specified in the publication Kitasatospora aureofaciens
1.06
-
3-methyl-2-oxopentanoate pH 10.4, temperature not specified in the publication Kitasatospora aureofaciens
1.2
-
2-oxobutanoate pH 10.4, temperature not specified in the publication Kitasatospora aureofaciens
1.66
-
L-norvaline pH 10.4, temperature not specified in the publication Kitasatospora aureofaciens
1.72
-
L-isoleucine pH 10.4, temperature not specified in the publication Kitasatospora aureofaciens
1.85
-
L-leucine pH 10.4, temperature not specified in the publication Kitasatospora aureofaciens
4.35
-
L-2-aminobutanoate pH 10.4, temperature not specified in the publication Kitasatospora aureofaciens
17.2
-
pyruvate pH 10.4, temperature not specified in the publication Kitasatospora aureofaciens
22.5
-
L-norleucine pH 10.4, temperature not specified in the publication Kitasatospora aureofaciens
333
-
L-alanine pH 10.4, temperature not specified in the publication Kitasatospora aureofaciens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
41000
-
6 * 41000, SDS-PAGE Kitasatospora aureofaciens
240000
-
gel filtration Kitasatospora aureofaciens

Organism

Organism UniProt Comment Textmining
Kitasatospora aureofaciens
-
-
-
Kitasatospora aureofaciens BS-5
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Kitasatospora aureofaciens

Storage Stability

Storage Stability Organism
-80°C, presence of 20% glycerol, stable for at least 2 months Kitasatospora aureofaciens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-oxobutanoate + NH3 + NADH + H+ 116% of the activity with 2-oxoisovalerate Kitasatospora aureofaciens L-2-aminobutanoate + H2O + NAD+
-
r
2-oxoisohexanoate + NH3 + NADH + H+ 26.5% of the activity with 2-oxoisovalerate Kitasatospora aureofaciens L-leucine + H2O + NAD+
-
r
2-oxoisovalerate + NH3 + NADH + H+
-
Kitasatospora aureofaciens L-valine + H2O + NAD+
-
r
3-methyl-2-oxopentanoate + NH3 + NADH + H+ 24.5% of the activity with 2-oxoisovalerate Kitasatospora aureofaciens L-isoleucine + H2O + NAD+
-
r
L-2-aminobutanoate + H2O + NAD+ 125% of the activity with L-valine Kitasatospora aureofaciens 2-oxobutanoate + NH3 + NADH + H+
-
r
L-alanine + H2O + NAD+ 4.5% of the activity with L-valine Kitasatospora aureofaciens pyruvate + NH3 + NADH + H+
-
r
L-isoleucine + H2O + NAD+ 34% of the activity with L-valine Kitasatospora aureofaciens 3-methyl-2-oxopentanoate + NH3 + NADH + H+
-
r
L-leucine + H2O + NAD+ 52.8% of the activity with L-valine Kitasatospora aureofaciens 2-oxoisohexanoate + NH3 + NADH + H+
-
r
L-norleucine + H2O + NAD+ 18.5% of the activity with L-valine Kitasatospora aureofaciens 2-oxohexanoate + NH3 + NADH + H+
-
r
L-norvaline + H2O + NAD+ 100% of the activity with L-valine Kitasatospora aureofaciens 2-oxovalerate + NH3 + NADH + H+
-
r
L-valine + H2O + NAD+
-
Kitasatospora aureofaciens 3-methyl-2-oxobutanoate + NH3 + NADH + H+
-
r
additional information no substrates: D-valine, glycine, L-threonine, L-serine, L-cysteine, L-glutamate, L-aspartate, L-asparagine, L-arginine, L-histidine, L-tryptophan, and DL-phenylalanine, and 2-ketoglutarate and oxalacetate Kitasatospora aureofaciens ?
-
?
pyruvate + H2O + NAD+ 20.8% of the activity with 2-oxoisovalerate Kitasatospora aureofaciens L-alanine + NH3 + NADH + H+
-
r

Subunits

Subunits Comment Organism
hexamer 6 * 41000, SDS-PAGE Kitasatospora aureofaciens

Synonyms

Synonyms Comment Organism
VDH2
-
Kitasatospora aureofaciens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
65
-
both reduxtive amination and oxidative deamination Kitasatospora aureofaciens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
reductive amination Kitasatospora aureofaciens
10.4
-
oxidative deamination Kitasatospora aureofaciens

Cofactor

Cofactor Comment Organism Structure
1,N6-etheno-NAD+ 62% of the activity with NAD+ Kitasatospora aureofaciens
deamino-NAD+ 373% of the activity with NAD+ Kitasatospora aureofaciens
additional information no cofactor: 3-acetylpyridine-NAD+, thionicotinamide-NAD+, alpha-NAD+, 3-pyridinaldehyde-NAD+, deamido-NAD+ Kitasatospora aureofaciens
NAD+
-
Kitasatospora aureofaciens
NADH
-
Kitasatospora aureofaciens
nicotinamide guanine dinucleotide 80% of the activity with NAD+ Kitasatospora aureofaciens