Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-hydroxymercuribenzoate | 0.01 mM, 100% inhibition | Kitasatospora aureofaciens | |
iodoacetamide | 1 mM, 50% inhibition | Kitasatospora aureofaciens | |
Mn2+ | 1 mM, 60% inhibition | Kitasatospora aureofaciens | |
additional information | activity is not significantly affected by 1 mM ATP, ADP, AMP, Mg2+, Ca2+, Cu2+, Zn2+, and metabolites of the citric acid cycle | Kitasatospora aureofaciens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.42 | - |
2-Oxoisohexanoate | pH 10.4, temperature not specified in the publication | Kitasatospora aureofaciens | |
0.46 | - |
2-oxoisovalerate | pH 10.4, temperature not specified in the publication | Kitasatospora aureofaciens | |
0.75 | - |
L-valine | pH 10.4, temperature not specified in the publication | Kitasatospora aureofaciens | |
1.06 | - |
3-methyl-2-oxopentanoate | pH 10.4, temperature not specified in the publication | Kitasatospora aureofaciens | |
1.2 | - |
2-oxobutanoate | pH 10.4, temperature not specified in the publication | Kitasatospora aureofaciens | |
1.66 | - |
L-norvaline | pH 10.4, temperature not specified in the publication | Kitasatospora aureofaciens | |
1.72 | - |
L-isoleucine | pH 10.4, temperature not specified in the publication | Kitasatospora aureofaciens | |
1.85 | - |
L-leucine | pH 10.4, temperature not specified in the publication | Kitasatospora aureofaciens | |
4.35 | - |
L-2-aminobutanoate | pH 10.4, temperature not specified in the publication | Kitasatospora aureofaciens | |
17.2 | - |
pyruvate | pH 10.4, temperature not specified in the publication | Kitasatospora aureofaciens | |
22.5 | - |
L-norleucine | pH 10.4, temperature not specified in the publication | Kitasatospora aureofaciens | |
333 | - |
L-alanine | pH 10.4, temperature not specified in the publication | Kitasatospora aureofaciens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
41000 | - |
6 * 41000, SDS-PAGE | Kitasatospora aureofaciens |
240000 | - |
gel filtration | Kitasatospora aureofaciens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Kitasatospora aureofaciens | - |
- |
- |
Kitasatospora aureofaciens BS-5 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Kitasatospora aureofaciens |
Storage Stability | Organism |
---|---|
-80°C, presence of 20% glycerol, stable for at least 2 months | Kitasatospora aureofaciens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxobutanoate + NH3 + NADH + H+ | 116% of the activity with 2-oxoisovalerate | Kitasatospora aureofaciens | L-2-aminobutanoate + H2O + NAD+ | - |
r | |
2-oxoisohexanoate + NH3 + NADH + H+ | 26.5% of the activity with 2-oxoisovalerate | Kitasatospora aureofaciens | L-leucine + H2O + NAD+ | - |
r | |
2-oxoisovalerate + NH3 + NADH + H+ | - |
Kitasatospora aureofaciens | L-valine + H2O + NAD+ | - |
r | |
3-methyl-2-oxopentanoate + NH3 + NADH + H+ | 24.5% of the activity with 2-oxoisovalerate | Kitasatospora aureofaciens | L-isoleucine + H2O + NAD+ | - |
r | |
L-2-aminobutanoate + H2O + NAD+ | 125% of the activity with L-valine | Kitasatospora aureofaciens | 2-oxobutanoate + NH3 + NADH + H+ | - |
r | |
L-alanine + H2O + NAD+ | 4.5% of the activity with L-valine | Kitasatospora aureofaciens | pyruvate + NH3 + NADH + H+ | - |
r | |
L-isoleucine + H2O + NAD+ | 34% of the activity with L-valine | Kitasatospora aureofaciens | 3-methyl-2-oxopentanoate + NH3 + NADH + H+ | - |
r | |
L-leucine + H2O + NAD+ | 52.8% of the activity with L-valine | Kitasatospora aureofaciens | 2-oxoisohexanoate + NH3 + NADH + H+ | - |
r | |
L-norleucine + H2O + NAD+ | 18.5% of the activity with L-valine | Kitasatospora aureofaciens | 2-oxohexanoate + NH3 + NADH + H+ | - |
r | |
L-norvaline + H2O + NAD+ | 100% of the activity with L-valine | Kitasatospora aureofaciens | 2-oxovalerate + NH3 + NADH + H+ | - |
r | |
L-valine + H2O + NAD+ | - |
Kitasatospora aureofaciens | 3-methyl-2-oxobutanoate + NH3 + NADH + H+ | - |
r | |
additional information | no substrates: D-valine, glycine, L-threonine, L-serine, L-cysteine, L-glutamate, L-aspartate, L-asparagine, L-arginine, L-histidine, L-tryptophan, and DL-phenylalanine, and 2-ketoglutarate and oxalacetate | Kitasatospora aureofaciens | ? | - |
? | |
pyruvate + H2O + NAD+ | 20.8% of the activity with 2-oxoisovalerate | Kitasatospora aureofaciens | L-alanine + NH3 + NADH + H+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
hexamer | 6 * 41000, SDS-PAGE | Kitasatospora aureofaciens |
Synonyms | Comment | Organism |
---|---|---|
VDH2 | - |
Kitasatospora aureofaciens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
both reduxtive amination and oxidative deamination | Kitasatospora aureofaciens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
reductive amination | Kitasatospora aureofaciens |
10.4 | - |
oxidative deamination | Kitasatospora aureofaciens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
1,N6-etheno-NAD+ | 62% of the activity with NAD+ | Kitasatospora aureofaciens | |
deamino-NAD+ | 373% of the activity with NAD+ | Kitasatospora aureofaciens | |
additional information | no cofactor: 3-acetylpyridine-NAD+, thionicotinamide-NAD+, alpha-NAD+, 3-pyridinaldehyde-NAD+, deamido-NAD+ | Kitasatospora aureofaciens | |
NAD+ | - |
Kitasatospora aureofaciens | |
NADH | - |
Kitasatospora aureofaciens | |
nicotinamide guanine dinucleotide | 80% of the activity with NAD+ | Kitasatospora aureofaciens |