BRENDA - Enzyme Database
show all sequences of 1.4.1.23

Isolation and characterization of valine dehydrogenase from Streptomyces aureofaciens

Vancurova, I.; Vancura, A.; Volc, J.; Neuzil, J.; Flieger, M.; Basarova, G.; Behal, V.; J. Bacteriol. 170, 5192-5196 (1988) View publication on PubMedView publication on EuropePMC

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
Hg2+
HgCl2: 0.01 mM, 91% loss of activity
Kitasatospora aureofaciens
additional information
no inhibition by nucleotide mono-, di-, triphosphates or the free bases adenine, cytosine, thymine, guanine and uracil
Kitasatospora aureofaciens
p-chloromercuribenzoate
0.01 mM, 83% loss of activity
Kitasatospora aureofaciens
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.023
-
NADH
-
Kitasatospora aureofaciens
0.023
-
NAD+
pH 10.4, 30°C
Kitasatospora aureofaciens
0.1
-
NAD+
-
Kitasatospora aureofaciens
0.8
-
2-oxoisocaproate
-
Kitasatospora aureofaciens
0.8
-
2-Oxoisohexanoate
pH 10.4, 30°C
Kitasatospora aureofaciens
1.25
-
2-oxoisovalerate
-
Kitasatospora aureofaciens
1.3
-
2-oxoisovalerate
pH 10.4, 30°C
Kitasatospora aureofaciens
1.5
-
2-oxobutyrate
-
Kitasatospora aureofaciens
1.5
-
2-oxobutanoate
pH 10.4, 30°C
Kitasatospora aureofaciens
2.5
-
L-valine
-
Kitasatospora aureofaciens
2.5
-
L-valine
pH 10.4, 30°C
Kitasatospora aureofaciens
5
-
L-isoleucine
-
Kitasatospora aureofaciens
5
-
L-isoleucine
pH 10.4, 30°C
Kitasatospora aureofaciens
5.7
-
L-norvaline
-
Kitasatospora aureofaciens
5.7
-
L-norvaline
pH 10.4, 30°C
Kitasatospora aureofaciens
6.3
-
L-leucine
-
Kitasatospora aureofaciens
6.3
-
L-leucine
pH 10.4, 30°C
Kitasatospora aureofaciens
6.7
-
2-oxo-3-methylvalerate
-
Kitasatospora aureofaciens
6.7
-
3-methyl-2-oxopentanoate
pH 10.4, 30°C
Kitasatospora aureofaciens
14.8
-
L-2-aminobutyrate
-
Kitasatospora aureofaciens
14.8
-
L-2-aminobutanoate
pH 10.4, 30°C
Kitasatospora aureofaciens
15.3
-
pyruvate
-
Kitasatospora aureofaciens
15.3
-
pyruvate
pH 10.4, 30°C
Kitasatospora aureofaciens
15.6
-
L-norleucine
-
Kitasatospora aureofaciens
15.6
-
L-norleucine
pH 10.4, 30°C
Kitasatospora aureofaciens
18.2
-
NH4+
-
Kitasatospora aureofaciens
18.2
-
NH3
pH 10.4, 30°C
Kitasatospora aureofaciens
333
-
L-alanine
pH 10.4, 30°C
Kitasatospora aureofaciens
333.6
-
L-alanine
-
Kitasatospora aureofaciens
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
29000
-
4 * 29000, SDS-PAGE
Kitasatospora aureofaciens
116000
-
equilibrium ultracentrifugation
Kitasatospora aureofaciens
116000
118000
gel filtration and equilibrium ultracentrifugation
Kitasatospora aureofaciens
118000
-
gel filtration
Kitasatospora aureofaciens
Organism
Organism
UniProt
Commentary
Textmining
Kitasatospora aureofaciens
-
-
-
Kitasatospora aureofaciens
-
a UV mutant derived from the high-producing strain 84/25
-
Kitasatospora aureofaciens 50/137
-
a UV mutant derived from the high-producing strain 84/25
-
Purification (Commentary)
Purification (Commentary)
Organism
-
Kitasatospora aureofaciens
785fold purification
Kitasatospora aureofaciens
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
-
Kitasatospora aureofaciens
Storage Stability
Storage Stability
Organism
-25°C, 0.2 M Tris-HCl buffer, pH 7.4, 2 months, no loss of activity
Kitasatospora aureofaciens
4°C, 70% (NH4)2SO4 suspension, 3 weeks, no loss of activity
Kitasatospora aureofaciens
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
2-oxobutanoate + NH3 + NADH + H+
48.2% of the activity with 2-oxoisovalerate
391695
Kitasatospora aureofaciens
L-2-aminobutanoate + H2O + NAD+
-
-
-
r
2-oxoisohexanoate + NH3 + NADH + H+
38.1% of the activity with 2-oxoisovalerate
391695
Kitasatospora aureofaciens
L-leucine + H2O + NAD+
-
-
-
r
2-oxoisovalerate + NH3 + NADH + H+
-
391695
Kitasatospora aureofaciens
L-valine + H2O + NAD+
-
-
-
r
3-methyl-2-oxopentanoate + NH3 + NADH + H+
22.3% of the activity with 2-oxoisovalerate
391695
Kitasatospora aureofaciens
L-isoleucine + H2O + NAD+
-
-
-
r
L-2-aminobutanoate + H2O + NAD+
16.5% of the activity with L-valine
391695
Kitasatospora aureofaciens
2-oxobutanoate + NH3 + NADH + H+
-
-
-
r
L-2-aminobutyrate + H2O + NAD+
16.5% of the activity with L-valine
391695
Kitasatospora aureofaciens
2-oxobutyrate + NADH + NH3
reductive amination: 48.2% of the activity with 2-oxoisovalerate
391695
Kitasatospora aureofaciens
r
L-2-aminobutyrate + H2O + NAD+
L-2-aminobutyrate is identical with L-alpha-aminobutyrate
391695
Kitasatospora aureofaciens
2-oxobutyrate + NADH + NH3
reductive amination: 48.2% of the activity with 2-oxoisovalerate
391695
Kitasatospora aureofaciens
r
L-2-aminobutyrate + H2O + NAD+
16.5% of the activity with L-valine
391695
Kitasatospora aureofaciens 50/137
2-oxobutyrate + NADH + NH3
reductive amination: 48.2% of the activity with 2-oxoisovalerate
391695
Kitasatospora aureofaciens 50/137
r
L-alanine + H2O + NAD+
very poor substrate: 1.2% of the activity with L-valine
391695
Kitasatospora aureofaciens
pyruvate + NH3 + NADH
pyruvate is identical with alpha-ketopropanoate and 2-oxopropanoate
391695
Kitasatospora aureofaciens
r
L-alanine + H2O + NAD+
very poor substrate: 1.2% of the activity with L-valine
391695
Kitasatospora aureofaciens
pyruvate + NH3 + NADH
reductive amination: very poor substrate, 1.3% of the activity with 2-oxoisovalerate
391695
Kitasatospora aureofaciens
r
L-alanine + H2O + NAD+
1.9% of the activity with L-valine
391695
Kitasatospora aureofaciens
pyruvate + NH3 + NADH + H+
-
-
-
r
L-isoleucine + H2O + NAD+
46.5% of the activity with L-valine
391695
Kitasatospora aureofaciens
3-methyl-2-oxopentanoate + NADH + NH3
2-oxo-3-methylvalerate is identical with alpha-keto-beta-methylpentanoate
391695
Kitasatospora aureofaciens
r
L-isoleucine + H2O + NAD+
46.5% of the activity with L-valine
391695
Kitasatospora aureofaciens
3-methyl-2-oxopentanoate + NADH + NH3
reductive amination: 22.3% of the activity with 2-oxoisovalerate
391695
Kitasatospora aureofaciens
r
L-isoleucine + H2O + NAD+
46.5% of the activity with L-valine
391695
Kitasatospora aureofaciens
3-methyl-2-oxopentanoate + NH3 + NADH + H+
-
-
-
r
L-leucine + H2O + NAD+
36% of the activity with L-valine
391695
Kitasatospora aureofaciens
2-oxoisocaproate + NADH + NH3
2-oxoisocaproate is identical with 2-oxo-4-methylpentanoate, 2-oxoisohexanoate and alpha-ketoisocaproate
391695
Kitasatospora aureofaciens
r
L-leucine + H2O + NAD+
36% of the activity with L-valine
391695
Kitasatospora aureofaciens
2-oxoisocaproate + NADH + NH3
reductive amination: 38.1% of the activity with 2-oxoisovalerate
391695
Kitasatospora aureofaciens
r
L-leucine + H2O + NAD+
36% of the activity with L-valine
391695
Kitasatospora aureofaciens
2-oxoisohexanoate + NH3 + NADH + H+
-
-
-
r
L-norleucine + H2O + NAD+
10.5% of the activity with L-valine
391695
Kitasatospora aureofaciens
2-oxocaproate + NADH + NH3
-
-
-
r
L-norleucine + H2O + NAD+
10.5% of the activity with L-valine
391695
Kitasatospora aureofaciens
2-oxohexanoate + NH3 + NADH + H+
-
-
-
r
L-norvaline + H2O + NAD+
43% of the activity with L-valine
391695
Kitasatospora aureofaciens
2-oxovalerate + NH3 + NADH
-
-
-
r
L-norvaline + H2O + NAD+
43% of the activity with L-valine
391695
Kitasatospora aureofaciens 50/137
2-oxovalerate + NH3 + NADH
-
-
-
r
L-norvaline + H2O + NAD+
43.0% of the activity with L-valine
391695
Kitasatospora aureofaciens
2-oxovalerate + NH3 + NADH + H+
-
-
-
r
L-valine + H2O + NAD+
-
391695
Kitasatospora aureofaciens
3-methyl-2-oxobutanoate + NH3 + NADH + H+
-
-
-
r
L-valine + NAD(P)+ + H2O
NAD+
391695
Kitasatospora aureofaciens
2-oxoisovalerate + NH3 + NAD(P)H
NADH
391695
Kitasatospora aureofaciens
r
L-valine + NAD(P)+ + H2O
NAD+
391695
Kitasatospora aureofaciens
2-oxoisovalerate + NH3 + NAD(P)H
2-oxoisovalerate is identical with 3-methyl-2-oxobutanoate and alpha-ketoisovalerate
391695
Kitasatospora aureofaciens
r
L-valine + NAD(P)+ + H2O
NAD+
391695
Kitasatospora aureofaciens
2-oxoisovalerate + NH3 + NAD(P)H
highest activity with 2-oxoisovalerate
391695
Kitasatospora aureofaciens
r
L-valine + NAD(P)+ + H2O
NAD+
391695
Kitasatospora aureofaciens 50/137
2-oxoisovalerate + NH3 + NAD(P)H
NADH
391695
Kitasatospora aureofaciens 50/137
r
L-valine + NAD(P)+ + H2O
NAD+
391695
Kitasatospora aureofaciens 50/137
2-oxoisovalerate + NH3 + NAD(P)H
2-oxoisovalerate is identical with 3-methyl-2-oxobutanoate and alpha-ketoisovalerate
391695
Kitasatospora aureofaciens 50/137
r
L-valine + NAD(P)+ + H2O
NAD+
391695
Kitasatospora aureofaciens 50/137
2-oxoisovalerate + NH3 + NAD(P)H
highest activity with 2-oxoisovalerate
391695
Kitasatospora aureofaciens 50/137
r
pyruvate + H2O + NAD+
1.3% of the activity with 2-oxoisovalerate
391695
Kitasatospora aureofaciens
L-alanine + NH3 + NADH + H+
-
-
-
r
Subunits
Subunits
Commentary
Organism
tetramer
4 * 29000, SDS-PAGE
Kitasatospora aureofaciens
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
assay at
Kitasatospora aureofaciens
65
-
-
Kitasatospora aureofaciens
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9
-
reductive amination
Kitasatospora aureofaciens
9
-
reductive amination of 2-oxoisovalerate, Tris-HCl buffer
Kitasatospora aureofaciens
10.7
-
oxidative deamination
Kitasatospora aureofaciens
10.7
-
oxidative deamination of L-valine, glycine-KCl-KOH buffer
Kitasatospora aureofaciens
Cofactor
Cofactor
Commentary
Organism
Structure
1,N6-etheno-NAD+
58.4% of the activity with NAD+
Kitasatospora aureofaciens
3-acetylpyridine-NAD+
100% of the activity with NAD+
Kitasatospora aureofaciens
3-pyridinealdehyde-NAD+
37.4% of the activity with NAD+
Kitasatospora aureofaciens
deamino-NAD+
83.7% of the activity with NAD+
Kitasatospora aureofaciens
additional information
no cofactor: NADP+
Kitasatospora aureofaciens
NAD+
-
Kitasatospora aureofaciens
NAD+
NAD+ required, no use of NADP+
Kitasatospora aureofaciens
NADH
-
Kitasatospora aureofaciens
NADP+
only 8.4% of the activity with NAD+
Kitasatospora aureofaciens
nicotinamide guanine dinucleotide
72.3% of the activity with NAD+
Kitasatospora aureofaciens
pI Value
Organism
Commentary
pI Value Maximum
pI Value
Kitasatospora aureofaciens
isoelectric focusing
-
5.1
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
1,N6-etheno-NAD+
58.4% of the activity with NAD+
Kitasatospora aureofaciens
3-acetylpyridine-NAD+
100% of the activity with NAD+
Kitasatospora aureofaciens
3-pyridinealdehyde-NAD+
37.4% of the activity with NAD+
Kitasatospora aureofaciens
deamino-NAD+
83.7% of the activity with NAD+
Kitasatospora aureofaciens
additional information
no cofactor: NADP+
Kitasatospora aureofaciens
NAD+
-
Kitasatospora aureofaciens
NAD+
NAD+ required, no use of NADP+
Kitasatospora aureofaciens
NADH
-
Kitasatospora aureofaciens
NADP+
only 8.4% of the activity with NAD+
Kitasatospora aureofaciens
nicotinamide guanine dinucleotide
72.3% of the activity with NAD+
Kitasatospora aureofaciens
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Hg2+
HgCl2: 0.01 mM, 91% loss of activity
Kitasatospora aureofaciens
additional information
no inhibition by nucleotide mono-, di-, triphosphates or the free bases adenine, cytosine, thymine, guanine and uracil
Kitasatospora aureofaciens
p-chloromercuribenzoate
0.01 mM, 83% loss of activity
Kitasatospora aureofaciens
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.023
-
NADH
-
Kitasatospora aureofaciens
0.023
-
NAD+
pH 10.4, 30°C
Kitasatospora aureofaciens
0.1
-
NAD+
-
Kitasatospora aureofaciens
0.8
-
2-oxoisocaproate
-
Kitasatospora aureofaciens
0.8
-
2-Oxoisohexanoate
pH 10.4, 30°C
Kitasatospora aureofaciens
1.25
-
2-oxoisovalerate
-
Kitasatospora aureofaciens
1.3
-
2-oxoisovalerate
pH 10.4, 30°C
Kitasatospora aureofaciens
1.5
-
2-oxobutyrate
-
Kitasatospora aureofaciens
1.5
-
2-oxobutanoate
pH 10.4, 30°C
Kitasatospora aureofaciens
2.5
-
L-valine
-
Kitasatospora aureofaciens
2.5
-
L-valine
pH 10.4, 30°C
Kitasatospora aureofaciens
5
-
L-isoleucine
-
Kitasatospora aureofaciens
5
-
L-isoleucine
pH 10.4, 30°C
Kitasatospora aureofaciens
5.7
-
L-norvaline
-
Kitasatospora aureofaciens
5.7
-
L-norvaline
pH 10.4, 30°C
Kitasatospora aureofaciens
6.3
-
L-leucine
-
Kitasatospora aureofaciens
6.3
-
L-leucine
pH 10.4, 30°C
Kitasatospora aureofaciens
6.7
-
2-oxo-3-methylvalerate
-
Kitasatospora aureofaciens
6.7
-
3-methyl-2-oxopentanoate
pH 10.4, 30°C
Kitasatospora aureofaciens
14.8
-
L-2-aminobutyrate
-
Kitasatospora aureofaciens
14.8
-
L-2-aminobutanoate
pH 10.4, 30°C
Kitasatospora aureofaciens
15.3
-
pyruvate
-
Kitasatospora aureofaciens
15.3
-
pyruvate
pH 10.4, 30°C
Kitasatospora aureofaciens
15.6
-
L-norleucine
-
Kitasatospora aureofaciens
15.6
-
L-norleucine
pH 10.4, 30°C
Kitasatospora aureofaciens
18.2
-
NH4+
-
Kitasatospora aureofaciens
18.2
-
NH3
pH 10.4, 30°C
Kitasatospora aureofaciens
333
-
L-alanine
pH 10.4, 30°C
Kitasatospora aureofaciens
333.6
-
L-alanine
-
Kitasatospora aureofaciens
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
29000
-
4 * 29000, SDS-PAGE
Kitasatospora aureofaciens
116000
-
equilibrium ultracentrifugation
Kitasatospora aureofaciens
116000
118000
gel filtration and equilibrium ultracentrifugation
Kitasatospora aureofaciens
118000
-
gel filtration
Kitasatospora aureofaciens
Purification (Commentary) (protein specific)
Commentary
Organism
-
Kitasatospora aureofaciens
785fold purification
Kitasatospora aureofaciens
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
-
Kitasatospora aureofaciens
Storage Stability (protein specific)
Storage Stability
Organism
-25°C, 0.2 M Tris-HCl buffer, pH 7.4, 2 months, no loss of activity
Kitasatospora aureofaciens
4°C, 70% (NH4)2SO4 suspension, 3 weeks, no loss of activity
Kitasatospora aureofaciens
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
2-oxobutanoate + NH3 + NADH + H+
48.2% of the activity with 2-oxoisovalerate
391695
Kitasatospora aureofaciens
L-2-aminobutanoate + H2O + NAD+
-
-
-
r
2-oxoisohexanoate + NH3 + NADH + H+
38.1% of the activity with 2-oxoisovalerate
391695
Kitasatospora aureofaciens
L-leucine + H2O + NAD+
-
-
-
r
2-oxoisovalerate + NH3 + NADH + H+
-
391695
Kitasatospora aureofaciens
L-valine + H2O + NAD+
-
-
-
r
3-methyl-2-oxopentanoate + NH3 + NADH + H+
22.3% of the activity with 2-oxoisovalerate
391695
Kitasatospora aureofaciens
L-isoleucine + H2O + NAD+
-
-
-
r
L-2-aminobutanoate + H2O + NAD+
16.5% of the activity with L-valine
391695
Kitasatospora aureofaciens
2-oxobutanoate + NH3 + NADH + H+
-
-
-
r
L-2-aminobutyrate + H2O + NAD+
16.5% of the activity with L-valine
391695
Kitasatospora aureofaciens
2-oxobutyrate + NADH + NH3
reductive amination: 48.2% of the activity with 2-oxoisovalerate
391695
Kitasatospora aureofaciens
r
L-2-aminobutyrate + H2O + NAD+
L-2-aminobutyrate is identical with L-alpha-aminobutyrate
391695
Kitasatospora aureofaciens
2-oxobutyrate + NADH + NH3
reductive amination: 48.2% of the activity with 2-oxoisovalerate
391695
Kitasatospora aureofaciens
r
L-2-aminobutyrate + H2O + NAD+
16.5% of the activity with L-valine
391695
Kitasatospora aureofaciens 50/137
2-oxobutyrate + NADH + NH3
reductive amination: 48.2% of the activity with 2-oxoisovalerate
391695
Kitasatospora aureofaciens 50/137
r
L-alanine + H2O + NAD+
very poor substrate: 1.2% of the activity with L-valine
391695
Kitasatospora aureofaciens
pyruvate + NH3 + NADH
pyruvate is identical with alpha-ketopropanoate and 2-oxopropanoate
391695
Kitasatospora aureofaciens
r
L-alanine + H2O + NAD+
very poor substrate: 1.2% of the activity with L-valine
391695
Kitasatospora aureofaciens
pyruvate + NH3 + NADH
reductive amination: very poor substrate, 1.3% of the activity with 2-oxoisovalerate
391695
Kitasatospora aureofaciens
r
L-alanine + H2O + NAD+
1.9% of the activity with L-valine
391695
Kitasatospora aureofaciens
pyruvate + NH3 + NADH + H+
-
-
-
r
L-isoleucine + H2O + NAD+
46.5% of the activity with L-valine
391695
Kitasatospora aureofaciens
3-methyl-2-oxopentanoate + NADH + NH3
2-oxo-3-methylvalerate is identical with alpha-keto-beta-methylpentanoate
391695
Kitasatospora aureofaciens
r
L-isoleucine + H2O + NAD+
46.5% of the activity with L-valine
391695
Kitasatospora aureofaciens
3-methyl-2-oxopentanoate + NADH + NH3
reductive amination: 22.3% of the activity with 2-oxoisovalerate
391695
Kitasatospora aureofaciens
r
L-isoleucine + H2O + NAD+
46.5% of the activity with L-valine
391695
Kitasatospora aureofaciens
3-methyl-2-oxopentanoate + NH3 + NADH + H+
-
-
-
r
L-leucine + H2O + NAD+
36% of the activity with L-valine
391695
Kitasatospora aureofaciens
2-oxoisocaproate + NADH + NH3
2-oxoisocaproate is identical with 2-oxo-4-methylpentanoate, 2-oxoisohexanoate and alpha-ketoisocaproate
391695
Kitasatospora aureofaciens
r
L-leucine + H2O + NAD+
36% of the activity with L-valine
391695
Kitasatospora aureofaciens
2-oxoisocaproate + NADH + NH3
reductive amination: 38.1% of the activity with 2-oxoisovalerate
391695
Kitasatospora aureofaciens
r
L-leucine + H2O + NAD+
36% of the activity with L-valine
391695
Kitasatospora aureofaciens
2-oxoisohexanoate + NH3 + NADH + H+
-
-
-
r
L-norleucine + H2O + NAD+
10.5% of the activity with L-valine
391695
Kitasatospora aureofaciens
2-oxocaproate + NADH + NH3
-
-
-
r
L-norleucine + H2O + NAD+
10.5% of the activity with L-valine
391695
Kitasatospora aureofaciens
2-oxohexanoate + NH3 + NADH + H+
-
-
-
r
L-norvaline + H2O + NAD+
43% of the activity with L-valine
391695
Kitasatospora aureofaciens
2-oxovalerate + NH3 + NADH
-
-
-
r
L-norvaline + H2O + NAD+
43% of the activity with L-valine
391695
Kitasatospora aureofaciens 50/137
2-oxovalerate + NH3 + NADH
-
-
-
r
L-norvaline + H2O + NAD+
43.0% of the activity with L-valine
391695
Kitasatospora aureofaciens
2-oxovalerate + NH3 + NADH + H+
-
-
-
r
L-valine + H2O + NAD+
-
391695
Kitasatospora aureofaciens
3-methyl-2-oxobutanoate + NH3 + NADH + H+
-
-
-
r
L-valine + NAD(P)+ + H2O
NAD+
391695
Kitasatospora aureofaciens
2-oxoisovalerate + NH3 + NAD(P)H
NADH
391695
Kitasatospora aureofaciens
r
L-valine + NAD(P)+ + H2O
NAD+
391695
Kitasatospora aureofaciens
2-oxoisovalerate + NH3 + NAD(P)H
2-oxoisovalerate is identical with 3-methyl-2-oxobutanoate and alpha-ketoisovalerate
391695
Kitasatospora aureofaciens
r
L-valine + NAD(P)+ + H2O
NAD+
391695
Kitasatospora aureofaciens
2-oxoisovalerate + NH3 + NAD(P)H
highest activity with 2-oxoisovalerate
391695
Kitasatospora aureofaciens
r
L-valine + NAD(P)+ + H2O
NAD+
391695
Kitasatospora aureofaciens 50/137
2-oxoisovalerate + NH3 + NAD(P)H
NADH
391695
Kitasatospora aureofaciens 50/137
r
L-valine + NAD(P)+ + H2O
NAD+
391695
Kitasatospora aureofaciens 50/137
2-oxoisovalerate + NH3 + NAD(P)H
2-oxoisovalerate is identical with 3-methyl-2-oxobutanoate and alpha-ketoisovalerate
391695
Kitasatospora aureofaciens 50/137
r
L-valine + NAD(P)+ + H2O
NAD+
391695
Kitasatospora aureofaciens 50/137
2-oxoisovalerate + NH3 + NAD(P)H
highest activity with 2-oxoisovalerate
391695
Kitasatospora aureofaciens 50/137
r
pyruvate + H2O + NAD+
1.3% of the activity with 2-oxoisovalerate
391695
Kitasatospora aureofaciens
L-alanine + NH3 + NADH + H+
-
-
-
r
Subunits (protein specific)
Subunits
Commentary
Organism
tetramer
4 * 29000, SDS-PAGE
Kitasatospora aureofaciens
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
assay at
Kitasatospora aureofaciens
65
-
-
Kitasatospora aureofaciens
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9
-
reductive amination
Kitasatospora aureofaciens
9
-
reductive amination of 2-oxoisovalerate, Tris-HCl buffer
Kitasatospora aureofaciens
10.7
-
oxidative deamination
Kitasatospora aureofaciens
10.7
-
oxidative deamination of L-valine, glycine-KCl-KOH buffer
Kitasatospora aureofaciens
pI Value (protein specific)
Organism
Commentary
pI Value Maximum
pI Value
Kitasatospora aureofaciens
isoelectric focusing
-
5.1
Other publictions for EC 1.4.1.23
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
391702
Nguyen
Purification and characterizat ...
Kitasatospora aureofaciens, Kitasatospora aureofaciens BS-5
Biochim. Biophys. Acta
1251
186-190
1995
-
-
-
-
-
-
4
12
-
-
2
-
-
3
-
-
1
-
-
-
-
1
13
1
1
1
-
-
-
2
-
-
6
-
-
-
-
-
-
6
-
-
-
-
4
-
12
-
-
2
-
-
-
-
1
-
-
-
1
13
1
1
-
-
-
2
-
-
-
-
-
-
-
-
-
391703
Nguyen
-
The tylosin producer, Streptom ...
Streptomyces fradiae
Microbiology
141
1139-1145
1995
-
-
-
-
-
-
-
5
-
-
2
-
-
1
-
-
1
-
-
-
-
2
13
1
1
1
-
-
-
2
-
-
6
-
-
-
-
-
-
6
-
-
-
-
-
-
5
-
-
2
-
-
-
-
1
-
-
-
2
13
1
1
-
-
-
2
-
-
-
2
-
-
2
-
-
717267
Ohshima
Valine dehydrogenase from a no ...
Alcaligenes faecalis
Biochim. Biophys. Acta
1162
221-226
1993
-
-
-
-
-
-
7
-
-
-
2
-
-
1
-
-
1
-
-
-
-
-
12
1
-
-
-
4
-
2
-
1
7
-
-
-
-
-
-
7
-
-
-
-
7
-
-
-
-
2
-
-
-
-
1
-
-
-
-
12
1
-
-
4
-
2
-
1
-
-
-
-
-
-
-
391693
Navarrete
Purification of an inducible L ...
Streptomyces coelicolor, Streptomyces coelicolor A3(2)
J. Gen. Microbiol.
136
273-281
1990
-
-
-
-
-
-
1
-
-
-
2
-
-
43
-
-
1
1
-
-
2
-
5
1
1
-
-
-
-
2
-
-
3
-
-
-
-
-
-
3
-
-
-
-
1
-
-
-
-
2
-
-
-
-
1
-
-
2
-
5
1
-
-
-
-
2
-
-
-
2
-
-
2
-
-
391694
Priestley
Purification and catalytic pro ...
Streptomyces cinnamonensis
Biochem. J.
261
853-861
1989
-
-
-
-
-
-
9
12
-
-
2
-
-
1
-
-
1
1
-
-
-
-
14
1
-
-
-
-
12
2
-
2
3
-
1
-
-
-
-
3
-
-
-
-
9
-
12
-
-
2
-
-
-
-
1
-
-
-
-
14
1
-
-
-
12
2
-
2
1
-
-
-
-
12
12
391690
Vancura
Valine dehydrogenase from Stre ...
Streptomyces fradiae
J. Gen. Microbiol.
134
3213-3219
1988
-
-
-
-
-
-
-
14
-
-
2
-
-
1
-
-
1
-
-
-
-
-
15
1
-
-
-
-
-
2
-
-
9
-
1
-
-
-
-
9
-
-
-
-
-
-
14
-
-
2
-
-
-
-
1
-
-
-
-
15
1
-
-
-
-
2
-
-
1
-
-
-
-
-
-
391695
Vancurova
Isolation and characterization ...
Kitasatospora aureofaciens, Kitasatospora aureofaciens 50/137
J. Bacteriol.
170
5192-5196
1988
-
-
-
-
-
-
3
29
-
-
4
-
-
3
-
-
2
-
-
-
1
2
30
1
-
2
-
-
-
4
-
-
10
-
1
-
-
-
-
10
-
-
-
-
3
-
29
-
-
4
-
-
-
-
2
-
-
1
2
30
1
2
-
-
-
4
-
-
1
-
-
-
-
-
-