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Literature summary for 1.4.1.23 extracted from

  • Priestley, N.D.; Robinson, J.A.
    Purification and catalytic properties of L-valine dehydrogenase from Streptomyces cinnamonensis (1989), Biochem. J., 261, 853-861.
    View publication on PubMedView publication on EuropePMC

Inhibitors

Inhibitors Comment Organism Structure
Ag+ 0.1 mM, strong inhibition Streptomyces virginiae
Ca2+ 1 mM, partial inhibition Streptomyces virginiae
Cu2+ 0.1 mM, strong inhibition Streptomyces virginiae
Hg+ 0.1 mM, strong inhibition Streptomyces virginiae
Hg2+ 0.1 mM, strong inhibition Streptomyces virginiae
Mg2+ 1 mM, partial inhibition Streptomyces virginiae
additional information not inhibitory: EDTA, benzamidine, PMSF, all at 5 mM, or citrate, cobalt(II), azide, Li+ and Mn2+, at 1 mM Streptomyces virginiae
p-chloromercuribenzoate 0.1 mM, strong inhibition Streptomyces virginiae
Zn2+ 1 mM, partial inhibition Streptomyces virginiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.13
-
2-oxobutanoate pH 9.5, 21°C Streptomyces virginiae
0.48
-
2-oxovalerate pH 9.5, 21°C Streptomyces virginiae
0.5
-
2-Oxoisohexanoate pH 9.5, 21°C Streptomyces virginiae
0.81
-
2-oxoisovalerate pH 9.5, 21°C Streptomyces virginiae
0.81
-
2-Oxohexanoate pH 9.5, 21°C Streptomyces virginiae
1.3
-
L-valine pH 10.0, 21°C Streptomyces virginiae
3.2
-
L-norvaline pH 10.0, 21°C Streptomyces virginiae
4
-
L-leucine pH 10.0, 21°C Streptomyces virginiae
10.8
-
L-isoleucine pH 10.0, 21°C Streptomyces virginiae
12.3
-
pyruvate pH 9.5, 21°C Streptomyces virginiae
19.6
-
L-2-aminobutanoate pH 10.0, 21°C Streptomyces virginiae
30
-
L-norleucine pH 10.0, 21°C Streptomyces virginiae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
41000
-
2 * 41000, SDS-PAGE Streptomyces virginiae
88000
-
gel filtration Streptomyces virginiae

Organism

Organism UniProt Comment Textmining
Streptomyces virginiae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Streptomyces virginiae

Reaction

Reaction Comment Organism Reaction ID
L-valine + H2O + NAD+ = 3-methyl-2-oxobutanoate + NH3 + NADH + H+ the reductive amination proceeds through a sequential ordered ternary-binary mechanism. NADH binds to the enzyme first, followed by 2-oxoisovalerate and NH3, and valine is released first, followed by NAD+ Streptomyces virginiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-oxobutanoate + NH3 + NADH + H+
-
Streptomyces virginiae L-2-aminobutanoate + H2O + NAD+
-
r
2-oxohexanoate + NH3 + NADH + H+
-
Streptomyces virginiae L-norleucine + H2O + NAD+
-
r
2-oxoisohexanoate + NH3 + NADH + H+
-
Streptomyces virginiae L-leucine + H2O + NAD+
-
r
2-oxoisovalerate + NH3 + NADH + H+
-
Streptomyces virginiae L-valine + H2O + NAD+ the 4'-pro-S hydrogen atom of NADH is transferred to the substrate in the reductive-amination reaction r
2-oxovalerate + H2O + NAD+
-
Streptomyces virginiae L-norvaline + NH3 + NADH + H+
-
r
3-methyl-2-oxopentanoate + NH3 + NADH + H+
-
Streptomyces virginiae L-isoleucine + H2O + NAD+
-
r
L-2-aminobutanoate + H2O + NAD+
-
Streptomyces virginiae 2-oxobutanoate + NH3 + NADH + H+
-
r
L-alanine + H2O + NAD+
-
Streptomyces virginiae pyruvate + NH3 + NADH + H+
-
r
L-isoleucine + H2O + NAD+
-
Streptomyces virginiae 3-methyl-2-oxopentanoate + NH3 + NADH + H+
-
r
L-leucine + H2O + NAD+
-
Streptomyces virginiae 2-oxoisohexanoate + NH3 + NADH + H+
-
r
L-norleucine + H2O + NAD+
-
Streptomyces virginiae 2-oxohexanoate + NH3 + NADH + H+
-
r
L-norvaline + H2O + NAD+
-
Streptomyces virginiae 2-oxovalerate + NH3 + NADH + H+
-
r
L-valine + H2O + NAD+
-
Streptomyces virginiae 3-methyl-2-oxobutanoate + NH3 + NADH + H+
-
r
pyruvate + NH3 + NADH + H+
-
Streptomyces virginiae L-alanine + H2O + NAD+
-
r

Subunits

Subunits Comment Organism
dimer 2 * 41000, SDS-PAGE Streptomyces virginiae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.8
-
L-isoleucine pH 10.0, 21°C Streptomyces virginiae
11.9
-
L-leucine pH 10.0, 21°C Streptomyces virginiae
17.9
-
L-norleucine pH 10.0, 21°C Streptomyces virginiae
18
-
L-norvaline pH 10.0, 21°C Streptomyces virginiae
28.3
-
L-valine pH 10.0, 21°C Streptomyces virginiae
71.9
-
2-Oxoisohexanoate pH 9.5, 21°C Streptomyces virginiae
99
-
2-oxobutanoate pH 9.5, 21°C Streptomyces virginiae
104
-
L-2-aminobutanoate pH 10.0, 21°C Streptomyces virginiae
107
-
2-Oxohexanoate pH 9.5, 21°C Streptomyces virginiae
114
-
pyruvate pH 9.5, 21°C Streptomyces virginiae
164
-
2-oxovalerate pH 9.5, 21°C Streptomyces virginiae
226
-
2-oxoisovalerate pH 9.5, 21°C Streptomyces virginiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.5
-
reductive amination Streptomyces virginiae
10
-
oxidative deamination Streptomyces virginiae

pH Stability

pH Stability pH Stability Maximum Comment Organism
7
-
dramatic decrease in stability below Streptomyces virginiae
7.4
-
most stable at Streptomyces virginiae

Cofactor

Cofactor Comment Organism Structure
additional information no cofactor: NADP+ Streptomyces virginiae
NAD+
-
Streptomyces virginiae
NADH
-
Streptomyces virginiae

pI Value

Organism Comment pI Value Maximum pI Value
Streptomyces virginiae isoelectric focusing
-
6.2

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.53
-
L-isoleucine pH 10.0, 21°C Streptomyces virginiae
0.6
-
L-norleucine pH 10.0, 21°C Streptomyces virginiae
3
-
L-leucine pH 10.0, 21°C Streptomyces virginiae
5.3
-
L-2-aminobutanoate pH 10.0, 21°C Streptomyces virginiae
5.6
-
L-norvaline pH 10.0, 21°C Streptomyces virginiae
9.3
-
pyruvate pH 9.5, 21°C Streptomyces virginiae
21.8
-
L-valine pH 10.0, 21°C Streptomyces virginiae
131
-
2-Oxohexanoate pH 9.5, 21°C Streptomyces virginiae
143
-
2-Oxoisohexanoate pH 9.5, 21°C Streptomyces virginiae
279
-
2-oxoisovalerate pH 9.5, 21°C Streptomyces virginiae
340
-
2-oxovalerate pH 9.5, 21°C Streptomyces virginiae
740
-
2-oxobutanoate pH 9.5, 21°C Streptomyces virginiae