Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.4.1.23 extracted from

  • Vancura, A.; Vancurova, I.; Volc, J.; Fussey, S.P.M.; Flieger, M.; Neuzil, J.; Marsalek, J.; Behal, V.
    Valine dehydrogenase from Streptomyces fradiae: purification and properties (1988), J. Gen. Microbiol., 134, 3213-3219.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.67
-
L-leucine pH 10.6, 30°C Streptomyces fradiae
0.8
-
2-oxoisovalerate pH 8.8, 30°C Streptomyces fradiae
0.82
-
2-Oxoisohexanoate pH 8.8, 30°C Streptomyces fradiae
1
-
2-oxobutanoate pH 8.8, 30°C Streptomyces fradiae
1
-
L-valine pH 10.6, 30°C Streptomyces fradiae
1.18
-
L-isoleucine pH 10.6, 30°C Streptomyces fradiae
1.33
-
L-norvaline pH 10.6, 30°C Streptomyces fradiae
1.94
-
2-oxovalerate pH 8.8, 30°C Streptomyces fradiae
2.23
-
2-Oxohexanoate pH 8.8, 30°C Streptomyces fradiae
3.07
-
L-2-aminobutanoate pH 10.6, 30°C Streptomyces fradiae
3.33
-
3-methyl-2-oxopentanoate pH 8.8, 30°C Streptomyces fradiae
6.68
-
L-norleucine pH 10.6, 30°C Streptomyces fradiae
25
-
pyruvate pH 8.8, 30°C Streptomyces fradiae
28.2
-
L-alanine pH 10.6, 30°C Streptomyces fradiae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
18000
-
12 * 18000, SDS-PAGE Streptomyces fradiae
215000 218000 gel filtration and equilibrium ultracentrifugation Streptomyces fradiae

Organism

Organism UniProt Comment Textmining
Streptomyces fradiae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Streptomyces fradiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-oxobutanoate + NH3 + NADH + H+ 78% of the activity with 2-oxoisovalerate Streptomyces fradiae L-2-aminobutanoate + H2O + NAD+
-
r
2-oxohexanoate + NH3 + NADH + H+ 43.8% of the activity with 2-oxoisovalerate Streptomyces fradiae L-norleucine + H2O + NAD+
-
r
2-oxoisohexanoate + NH3 + NADH + H+ 20% of the activity with 2-oxoisovalerate Streptomyces fradiae L-leucine + H2O + NAD+
-
r
2-oxoisovalerate + NH3 + NADH + H+
-
Streptomyces fradiae L-valine + H2O + NAD+
-
r
2-oxovalerate + H2O + NAD+ 79.5% of the activity with 2-oxoisovalerate Streptomyces fradiae L-norvaline + NH3 + NADH + H+
-
r
3-methyl-2-oxopentanoate + NH3 + NADH + H+ 23.2% of the activity with 2-oxoisovalerate Streptomyces fradiae L-isoleucine + H2O + NAD+
-
r
L-2-aminobutanoate + H2O + NAD+ 68.9% of the activity with L-valine Streptomyces fradiae 2-oxobutanoate + NH3 + NADH + H+
-
r
L-alanine + H2O + NAD+ 14.7% of the activity with L-valine Streptomyces fradiae pyruvate + NH3 + NADH + H+
-
r
L-isoleucine + H2O + NAD+ 29.4% of the activity with L-valine Streptomyces fradiae 3-methyl-2-oxopentanoate + NH3 + NADH + H+
-
r
L-leucine + H2O + NAD+ 24.5% of the activity with L-valine Streptomyces fradiae 2-oxoisohexanoate + NH3 + NADH + H+
-
r
L-norleucine + H2O + NAD+ 51.5% of the activity with L-valine Streptomyces fradiae 2-oxohexanoate + NH3 + NADH + H+
-
r
L-norvaline + H2O + NAD+ 98% of the activity with L-valine Streptomyces fradiae 2-oxovalerate + NH3 + NADH + H+
-
r
L-valine + H2O + NAD+
-
Streptomyces fradiae 3-methyl-2-oxobutanoate + NH3 + NADH + H+
-
r
additional information no substrates: D-valine, D-leucine, D-isoleucine, glycine, L-threonine, fialanine, L-serine, L-cysteine, L-methionine, L-glutamic acid, L-aspartic acid, L-asparagine, L-glutamine, L-lysine, L-phenylalanine, L-tyrosine, L-histidine and L-tryptophan as well as 2-oxoglutarate, oxalacetate, phenylpyruvate and glyoxalate Streptomyces fradiae ?
-
?
pyruvate + NH3 + NADH + H+ 31.6% of the activity with 2-oxoisovalerate Streptomyces fradiae L-alanine + H2O + NAD+
-
r

Subunits

Subunits Comment Organism
dodecamer 12 * 18000, SDS-PAGE Streptomyces fradiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.8
-
reductive amination Streptomyces fradiae
10.6
-
oxidative deamination Streptomyces fradiae

Cofactor

Cofactor Comment Organism Structure
1,N6-etheno-NAD+ 64.4% of the activity with NAD+ Streptomyces fradiae
3-acetylpyridine-NAD+ 64.5% of the activity with NAD+ Streptomyces fradiae
Alpha-NAD+ 3.6% of the activity with NAD+ Streptomyces fradiae
deamino-NAD+ 93.3% of the activity with NAD+ Streptomyces fradiae
additional information no cofactors: deamido-NAD+, 3-pyridinealdehyde-NAD+, thionicotinamide-NAD+ Streptomyces fradiae
NAD+
-
Streptomyces fradiae
NADH
-
Streptomyces fradiae
NADP+ 10.8% of the activity with NAD+ Streptomyces fradiae
nicotinamide guanine dinucleotide 92.9% of the activity with NAD+ Streptomyces fradiae

pI Value

Organism Comment pI Value Maximum pI Value
Streptomyces fradiae isoelectric focusing
-
4.7