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Literature summary for 1.4.1.21 extracted from

  • Wu, W.; Zhang, Y.; Liu, D.; Chen, Z.
    Efficient mining of natural NADH-utilizing dehydrogenases enables systematic cofactor engineering of lysine synthesis pathway of Corynebacterium glutamicum (2019), Metab. Eng., 52, 77-86 .
    View publication on PubMed
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Application

Application Comment Organism
synthesis individual overexpression of ASPDH, aspartate-semialdehyde dehydrogenase from Tistrella mobilis, dihydrodipicolinate reductase from Escherichia coli, and diaminopimelate dehydrogenase from Pseudothermotoga thermarum in Corynebacterium glutamicum LC298, a basic lysine producer, increases the production of lysine by 30.7%, 32.4%, 17.4%, and 36.8%, respectively. The highest increase of lysine production (30.7%) is observed for a triple-mutant strain (27.7 g/L, 0.35 g/g glucose) expressing ASPDH, aspartate-semialdehyde dehydrogenase from Tistrella mobilis, dihydrodipicolinate reductase from Escherichia coli. A quadruple-mutant strain expressing all of the four NADH-utilizing enzymes allows high lysine production (24.1 g/l, 0.30 g/g glucose) almost independent of the oxidative pentose phosphate pathway Pseudomonas aeruginosa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.042
-
 NADH pH 8.2, 37°C Pseudomonas aeruginosa
0.057
-
 NADPH pH 8.2, 37°C Pseudomonas aeruginosa

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
oxaloacetate + NH3 + NADH + H+
-
 Pseudomonas aeruginosa L-aspartate + H2O + NAD+
-
 ?
oxaloacetate + NH3 + NADPH + H+
-
 Pseudomonas aeruginosa L-aspartate + H2O + NADP+
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
68.4
-
 NADH pH 8.2, 37°C Pseudomonas aeruginosa
80.2
-
 NADPH pH 8.2, 37°C Pseudomonas aeruginosa

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1407
-
 NADPH pH 8.2, 37°C Pseudomonas aeruginosa
1629
-
 NADH pH 8.2, 37°C Pseudomonas aeruginosa