Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, expression of His-tagged enzyme in Escherichia coli | Bradyrhizobium japonicum |
DNA and amino acid sequence determination and analysis, expression of His-tagged enzyme in Escherichia coli | Rhodopseudomonas palustris |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.032 | - |
NADPH | pH 9.0, 28°C, recombinant enzyme | Bradyrhizobium japonicum | |
0.102 | - |
NADP+ | pH 9.8, 28°C, recombinant enzyme | Rhodopseudomonas palustris | |
0.12 | - |
NADP+ | pH 9.8, 28°C, recombinant enzyme | Bradyrhizobium japonicum | |
0.21 | - |
NADPH | pH 9.0, 28°C, recombinant enzyme | Rhodopseudomonas palustris | |
0.25 | - |
NADH | pH 9.0, 28°C, recombinant enzyme | Bradyrhizobium japonicum | |
4.3 | - |
NH3 | pH 9.0, 28°C, recombinant enzyme | Bradyrhizobium japonicum | |
4.5 | - |
NADH | pH 9.0, 28°C, recombinant enzyme | Rhodopseudomonas palustris | |
7 | - |
NAD+ | pH 9.8, 28°C, recombinant enzyme | Rhodopseudomonas palustris | |
9.2 | - |
oxaloacetate | pH 9.0, 28°C, recombinant enzyme | Rhodopseudomonas palustris | |
10 | - |
NAD+ | pH 9.8, 28°C, recombinant enzyme | Bradyrhizobium japonicum | |
11.3 | - |
NH3 | pH 9.0, 28°C, recombinant enzyme | Rhodopseudomonas palustris | |
13.4 | - |
L-aspartate | pH 9.8, 28°C, recombinant enzyme | Rhodopseudomonas palustris | |
21 | - |
oxaloacetate | pH 9.0, 28°C, recombinant enzyme | Bradyrhizobium japonicum | |
27 | - |
L-aspartate | pH 9.8, 28°C, recombinant enzyme | Bradyrhizobium japonicum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-aspartate + H2O + NAD(P)+ | Bradyrhizobium japonicum | - |
oxaloacetate + NH3 + NAD(P)H + H+ | - |
r | |
L-aspartate + H2O + NAD(P)+ | Rhodopseudomonas palustris | - |
oxaloacetate + NH3 + NAD(P)H + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bradyrhizobium japonicum | - |
- |
- |
Rhodopseudomonas palustris | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme 28fold from Escherichia coli by nickel affinity chromatography | Bradyrhizobium japonicum |
recombinant His-tagged enzyme 5fold from Escherichia coli by nickel affinity chromatography | Rhodopseudomonas palustris |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3.1 | - |
purified recombinant enzyme, with L-aspartate and NADP+, pH 9.8, 28°C | Rhodopseudomonas palustris |
4.2 | - |
purified recombinant enzyme, with L-aspartate and NADP+, pH 9.8, 28°C | Bradyrhizobium japonicum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-aspartate + H2O + NAD(P)+ | - |
Bradyrhizobium japonicum | oxaloacetate + NH3 + NAD(P)H + H+ | - |
r | |
L-aspartate + H2O + NAD(P)+ | - |
Rhodopseudomonas palustris | oxaloacetate + NH3 + NAD(P)H + H+ | - |
r | |
L-aspartate + H2O + NAD+ | - |
Bradyrhizobium japonicum | oxaloacetate + NH3 + NADH + H+ | - |
r | |
L-aspartate + H2O + NAD+ | - |
Rhodopseudomonas palustris | oxaloacetate + NH3 + NADH + H+ | - |
r | |
L-aspartate + H2O + NADP+ | - |
Bradyrhizobium japonicum | oxaloacetate + NH3 + NADPH + H+ | - |
r | |
L-aspartate + H2O + NADP+ | - |
Rhodopseudomonas palustris | oxaloacetate + NH3 + NADPH + H+ | - |
r | |
additional information | the enzyme catalyzes in vitro the reductive amination of oxaloacetate to L-aspartate by an order faster than the deamination reaction | Bradyrhizobium japonicum | ? | - |
? | |
additional information | the enzyme catalyzes in vitro the reductive amination of oxaloacetate to L-aspartate by an order faster than the deamination reaction | Rhodopseudomonas palustris | ? | - |
? | |
oxaloacetate + NH3 + NADH + H+ | - |
Bradyrhizobium japonicum | L-aspartate + H2O + NAD+ | - |
r | |
oxaloacetate + NH3 + NADH + H+ | - |
Rhodopseudomonas palustris | L-aspartate + H2O + NAD+ | - |
r | |
oxaloacetate + NH3 + NADPH + H+ | - |
Bradyrhizobium japonicum | L-aspartate + H2O + NADP+ | - |
r | |
oxaloacetate + NH3 + NADPH + H+ | - |
Rhodopseudomonas palustris | L-aspartate + H2O + NADP+ | - |
r |
Synonyms | Comment | Organism |
---|---|---|
L-aspartate dehydrogenase | - |
Bradyrhizobium japonicum |
L-aspartate dehydrogenase | - |
Rhodopseudomonas palustris |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
28 | - |
assay at | Bradyrhizobium japonicum |
28 | - |
assay at | Rhodopseudomonas palustris |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.59 | - |
NAD+ | pH 9.8, 28°C, recombinant enzyme | Rhodopseudomonas palustris | |
2.33 | - |
NADP+ | pH 9.8, 28°C, recombinant enzyme | Rhodopseudomonas palustris | |
2.86 | - |
L-aspartate | pH 9.8, 28°C, recombinant enzyme | Rhodopseudomonas palustris | |
3.3 | - |
NAD+ | pH 9.8, 28°C, recombinant enzyme | Bradyrhizobium japonicum | |
5.2 | - |
NADH | pH 9.0, 28°C, recombinant enzyme | Rhodopseudomonas palustris | |
6.2 | - |
NADP+ | pH 9.8, 28°C, recombinant enzyme | Bradyrhizobium japonicum | |
7.3 | - |
L-aspartate | pH 9.8, 28°C, recombinant enzyme | Bradyrhizobium japonicum | |
10.3 | - |
NH3 | pH 9.0, 28°C, recombinant enzyme | Rhodopseudomonas palustris | |
14.2 | - |
oxaloacetate | pH 9.0, 28°C, recombinant enzyme | Rhodopseudomonas palustris | |
19 | - |
NADPH | pH 9.0, 28°C, recombinant enzyme | Rhodopseudomonas palustris | |
24.7 | - |
NH3 | pH 9.0, 28°C, recombinant enzyme | Bradyrhizobium japonicum | |
26.2 | - |
NADH | pH 9.0, 28°C, recombinant enzyme | Bradyrhizobium japonicum | |
41 | - |
NADPH | pH 9.0, 28°C, recombinant enzyme | Bradyrhizobium japonicum | |
48 | - |
oxaloacetate | pH 9.0, 28°C, recombinant enzyme | Bradyrhizobium japonicum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | 9 | amination | Bradyrhizobium japonicum |
8 | 9 | amination | Rhodopseudomonas palustris |
9.8 | - |
deamination | Bradyrhizobium japonicum |
9.8 | - |
deamination | Rhodopseudomonas palustris |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Bradyrhizobium japonicum | |
NAD+ | - |
Rhodopseudomonas palustris | |
NADH | - |
Bradyrhizobium japonicum | |
NADH | - |
Rhodopseudomonas palustris | |
NADP+ | - |
Bradyrhizobium japonicum | |
NADP+ | - |
Rhodopseudomonas palustris | |
NADPH | - |
Bradyrhizobium japonicum | |
NADPH | - |
Rhodopseudomonas palustris |
General Information | Comment | Organism |
---|---|---|
physiological function | the amination activity of the enzyme may be important for the fixation of inorganic nitrogen | Bradyrhizobium japonicum |
physiological function | the amination activity of the enzyme may be important for the fixation of inorganic nitrogen | Rhodopseudomonas palustris |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.21 | - |
L-aspartate | pH 9.8, 28°C, recombinant enzyme | Rhodopseudomonas palustris | |
0.23 | - |
NAD+ | pH 9.8, 28°C, recombinant enzyme | Rhodopseudomonas palustris | |
0.28 | - |
L-aspartate | pH 9.8, 28°C, recombinant enzyme | Bradyrhizobium japonicum | |
0.33 | - |
NAD+ | pH 9.8, 28°C, recombinant enzyme | Bradyrhizobium japonicum | |
0.91 | - |
NH3 | pH 9.0, 28°C, recombinant enzyme | Rhodopseudomonas palustris | |
1.1 | - |
NADH | pH 9.0, 28°C, recombinant enzyme | Rhodopseudomonas palustris | |
1.5 | - |
oxaloacetate | pH 9.0, 28°C, recombinant enzyme | Rhodopseudomonas palustris | |
2.3 | - |
oxaloacetate | pH 9.0, 28°C, recombinant enzyme | Bradyrhizobium japonicum | |
5.7 | - |
NH3 | pH 9.0, 28°C, recombinant enzyme | Bradyrhizobium japonicum | |
23 | - |
NADP+ | pH 9.8, 28°C, recombinant enzyme | Rhodopseudomonas palustris | |
53 | - |
NADP+ | pH 9.8, 28°C, recombinant enzyme | Bradyrhizobium japonicum | |
90 | - |
NADPH | pH 9.0, 28°C, recombinant enzyme | Rhodopseudomonas palustris | |
100 | - |
NADH | pH 9.0, 28°C, recombinant enzyme | Bradyrhizobium japonicum | |
1300 | - |
NADPH | pH 9.0, 28°C, recombinant enzyme | Bradyrhizobium japonicum |