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Literature summary for 1.4.1.21 extracted from
- Novel NADPH-dependent L-aspartate dehydrogenases from the mesophilic nitrogen-fixing bacteria Rhodopseudomonas palustris and Bradyrhizobium japonicum (2013), Appl. Biochem. Microbiol., 49, 136-143.
No PubMed abstract available
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| DNA and amino acid sequence determination and analysis, expression of His-tagged enzyme in Escherichia coli | Bradyrhizobium japonicum |
| DNA and amino acid sequence determination and analysis, expression of His-tagged enzyme in Escherichia coli | Rhodopseudomonas palustris |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.032 | - |
NADPH | pH 9.0, 28°C, recombinant enzyme | Bradyrhizobium japonicum |  |
| 0.102 | - |
NADP+ | pH 9.8, 28°C, recombinant enzyme | Rhodopseudomonas palustris | |
| 0.12 | - |
NADP+ | pH 9.8, 28°C, recombinant enzyme | Bradyrhizobium japonicum | |
| 0.21 | - |
NADPH | pH 9.0, 28°C, recombinant enzyme | Rhodopseudomonas palustris | |
| 0.25 | - |
NADH | pH 9.0, 28°C, recombinant enzyme | Bradyrhizobium japonicum | |
| 4.3 | - |
NH3 | pH 9.0, 28°C, recombinant enzyme | Bradyrhizobium japonicum | |
| 4.5 | - |
NADH | pH 9.0, 28°C, recombinant enzyme | Rhodopseudomonas palustris | |
| 7 | - |
NAD+ | pH 9.8, 28°C, recombinant enzyme | Rhodopseudomonas palustris | |
| 9.2 | - |
oxaloacetate | pH 9.0, 28°C, recombinant enzyme | Rhodopseudomonas palustris | |
| 10 | - |
NAD+ | pH 9.8, 28°C, recombinant enzyme | Bradyrhizobium japonicum | |
| 11.3 | - |
NH3 | pH 9.0, 28°C, recombinant enzyme | Rhodopseudomonas palustris | |
| 13.4 | - |
L-aspartate | pH 9.8, 28°C, recombinant enzyme | Rhodopseudomonas palustris | |
| 21 | - |
oxaloacetate | pH 9.0, 28°C, recombinant enzyme | Bradyrhizobium japonicum | |
| 27 | - |
L-aspartate | pH 9.8, 28°C, recombinant enzyme | Bradyrhizobium japonicum | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-aspartate + H2O + NAD(P)+ | Bradyrhizobium japonicum | - |
oxaloacetate + NH3 + NAD(P)H + H+ | - |
r | |
| L-aspartate + H2O + NAD(P)+ | Rhodopseudomonas palustris | - |
oxaloacetate + NH3 + NAD(P)H + H+ | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bradyrhizobium japonicum | - |
- |
- |
| Rhodopseudomonas palustris | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged enzyme 28fold from Escherichia coli by nickel affinity chromatography | Bradyrhizobium japonicum |
| recombinant His-tagged enzyme 5fold from Escherichia coli by nickel affinity chromatography | Rhodopseudomonas palustris |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 3.1 | - |
purified recombinant enzyme, with L-aspartate and NADP+, pH 9.8, 28°C | Rhodopseudomonas palustris |
| 4.2 | - |
purified recombinant enzyme, with L-aspartate and NADP+, pH 9.8, 28°C | Bradyrhizobium japonicum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-aspartate + H2O + NAD(P)+ | - |
Bradyrhizobium japonicum | oxaloacetate + NH3 + NAD(P)H + H+ | - |
r | |
| L-aspartate + H2O + NAD(P)+ | - |
Rhodopseudomonas palustris | oxaloacetate + NH3 + NAD(P)H + H+ | - |
r | |
| L-aspartate + H2O + NAD+ | - |
Bradyrhizobium japonicum | oxaloacetate + NH3 + NADH + H+ | - |
r | |
| L-aspartate + H2O + NAD+ | - |
Rhodopseudomonas palustris | oxaloacetate + NH3 + NADH + H+ | - |
r | |
| L-aspartate + H2O + NADP+ | - |
Bradyrhizobium japonicum | oxaloacetate + NH3 + NADPH + H+ | - |
r | |
| L-aspartate + H2O + NADP+ | - |
Rhodopseudomonas palustris | oxaloacetate + NH3 + NADPH + H+ | - |
r | |
| additional information | the enzyme catalyzes in vitro the reductive amination of oxaloacetate to L-aspartate by an order faster than the deamination reaction | Bradyrhizobium japonicum | ? | - |
? | |
| additional information | the enzyme catalyzes in vitro the reductive amination of oxaloacetate to L-aspartate by an order faster than the deamination reaction | Rhodopseudomonas palustris | ? | - |
? | |
| oxaloacetate + NH3 + NADH + H+ | - |
Bradyrhizobium japonicum | L-aspartate + H2O + NAD+ | - |
r | |
| oxaloacetate + NH3 + NADH + H+ | - |
Rhodopseudomonas palustris | L-aspartate + H2O + NAD+ | - |
r | |
| oxaloacetate + NH3 + NADPH + H+ | - |
Bradyrhizobium japonicum | L-aspartate + H2O + NADP+ | - |
r | |
| oxaloacetate + NH3 + NADPH + H+ | - |
Rhodopseudomonas palustris | L-aspartate + H2O + NADP+ | - |
r | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| L-aspartate dehydrogenase | - |
Bradyrhizobium japonicum |
| L-aspartate dehydrogenase | - |
Rhodopseudomonas palustris |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 28 | - |
assay at | Bradyrhizobium japonicum |
| 28 | - |
assay at | Rhodopseudomonas palustris |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.59 | - |
NAD+ | pH 9.8, 28°C, recombinant enzyme | Rhodopseudomonas palustris | |
| 2.33 | - |
NADP+ | pH 9.8, 28°C, recombinant enzyme | Rhodopseudomonas palustris | |
| 2.86 | - |
L-aspartate | pH 9.8, 28°C, recombinant enzyme | Rhodopseudomonas palustris | |
| 3.3 | - |
NAD+ | pH 9.8, 28°C, recombinant enzyme | Bradyrhizobium japonicum | |
| 5.2 | - |
NADH | pH 9.0, 28°C, recombinant enzyme | Rhodopseudomonas palustris | |
| 6.2 | - |
NADP+ | pH 9.8, 28°C, recombinant enzyme | Bradyrhizobium japonicum | |
| 7.3 | - |
L-aspartate | pH 9.8, 28°C, recombinant enzyme | Bradyrhizobium japonicum | |
| 10.3 | - |
NH3 | pH 9.0, 28°C, recombinant enzyme | Rhodopseudomonas palustris | |
| 14.2 | - |
oxaloacetate | pH 9.0, 28°C, recombinant enzyme | Rhodopseudomonas palustris | |
| 19 | - |
NADPH | pH 9.0, 28°C, recombinant enzyme | Rhodopseudomonas palustris | |
| 24.7 | - |
NH3 | pH 9.0, 28°C, recombinant enzyme | Bradyrhizobium japonicum | |
| 26.2 | - |
NADH | pH 9.0, 28°C, recombinant enzyme | Bradyrhizobium japonicum | |
| 41 | - |
NADPH | pH 9.0, 28°C, recombinant enzyme | Bradyrhizobium japonicum | |
| 48 | - |
oxaloacetate | pH 9.0, 28°C, recombinant enzyme | Bradyrhizobium japonicum | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | 9 | amination | Bradyrhizobium japonicum |
| 8 | 9 | amination | Rhodopseudomonas palustris |
| 9.8 | - |
deamination | Bradyrhizobium japonicum |
| 9.8 | - |
deamination | Rhodopseudomonas palustris |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Bradyrhizobium japonicum | |
| NAD+ | - |
Rhodopseudomonas palustris | |
| NADH | - |
Bradyrhizobium japonicum | |
| NADH | - |
Rhodopseudomonas palustris | |
| NADP+ | - |
Bradyrhizobium japonicum | |
| NADP+ | - |
Rhodopseudomonas palustris | |
| NADPH | - |
Bradyrhizobium japonicum | |
| NADPH | - |
Rhodopseudomonas palustris | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | the amination activity of the enzyme may be important for the fixation of inorganic nitrogen | Bradyrhizobium japonicum |
| physiological function | the amination activity of the enzyme may be important for the fixation of inorganic nitrogen | Rhodopseudomonas palustris |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.21 | - |
L-aspartate | pH 9.8, 28°C, recombinant enzyme | Rhodopseudomonas palustris | |
| 0.23 | - |
NAD+ | pH 9.8, 28°C, recombinant enzyme | Rhodopseudomonas palustris | |
| 0.28 | - |
L-aspartate | pH 9.8, 28°C, recombinant enzyme | Bradyrhizobium japonicum | |
| 0.33 | - |
NAD+ | pH 9.8, 28°C, recombinant enzyme | Bradyrhizobium japonicum | |
| 0.91 | - |
NH3 | pH 9.0, 28°C, recombinant enzyme | Rhodopseudomonas palustris | |
| 1.1 | - |
NADH | pH 9.0, 28°C, recombinant enzyme | Rhodopseudomonas palustris | |
| 1.5 | - |
oxaloacetate | pH 9.0, 28°C, recombinant enzyme | Rhodopseudomonas palustris | |
| 2.3 | - |
oxaloacetate | pH 9.0, 28°C, recombinant enzyme | Bradyrhizobium japonicum | |
| 5.7 | - |
NH3 | pH 9.0, 28°C, recombinant enzyme | Bradyrhizobium japonicum | |
| 23 | - |
NADP+ | pH 9.8, 28°C, recombinant enzyme | Rhodopseudomonas palustris | |
| 53 | - |
NADP+ | pH 9.8, 28°C, recombinant enzyme | Bradyrhizobium japonicum | |
| 90 | - |
NADPH | pH 9.0, 28°C, recombinant enzyme | Rhodopseudomonas palustris | |
| 100 | - |
NADH | pH 9.0, 28°C, recombinant enzyme | Bradyrhizobium japonicum | |
| 1300 | - |
NADPH | pH 9.0, 28°C, recombinant enzyme | Bradyrhizobium japonicum | |
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