Literature summary for 1.4.1.20 extracted from

Seah, S.Y.; Britton, K.L.; Rice, D.W.; Asano, Y.; Engel, P.C.
Kinetic analysis of phenylalanine dehydrogenase mutants designed for aliphatic amino acid dehydrogenase activity with guidance from homology-based modelling (2003), Eur. J. Biochem., 270, 4628-4634.

Search for more literature for 1.4.1.20

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Lysinibacillus sphaericus

Protein Variants

Protein Variants	Comment	Organism
G124A	12% of wild-type kcat with L-phenylalanine	Lysinibacillus sphaericus
G124A/L307V	7.5% of wild-type kcat with L-phenylalanine	Lysinibacillus sphaericus
L307V	26% of wild-type kcat with L-phenylalanine	Lysinibacillus sphaericus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
39000	-	10 * 39000, SDS_PAGE	Lysinibacillus sphaericus
380000	-	gel filtration	Lysinibacillus sphaericus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
L-phenylalanine + H2O + NAD+	Lysinibacillus sphaericus	-	phenylpyruvate + NH3 + NADH	-	r

Organism

Organism	UniProt	Comment	Textmining
Lysinibacillus sphaericus	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant PheDH	Lysinibacillus sphaericus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
L-isoleucine + H2O + NAD+	-	Lysinibacillus sphaericus	3-methyl-2-oxopentanoate + NH3 + NADH	-	r
L-leucine + H2O + NAD+	-	Lysinibacillus sphaericus	3,3-dimethyl-2-oxo-butanoate + NH3 + NADH	-	r
L-methionine + H2O + NAD+	-	Lysinibacillus sphaericus	(4-methylsulfanyl)-2-oxobutanoate + NH3 + NADH	-	r
L-norleucine + H2O + NAD+	-	Lysinibacillus sphaericus	2-oxohexanoate + NH3 + NADH	-	r
L-norvaline + H2O + NAD+	-	Lysinibacillus sphaericus	2-oxopentanoate + NH3 + NADH	-	r
L-phenylalanine + H2O + NAD+	-	Lysinibacillus sphaericus	phenylpyruvate + NH3 + NADH	-	r
L-tyrosine + H2O + NAD+	-	Lysinibacillus sphaericus	(4-hydroxyphenyl)pyruvate + NH3 + NADH	-	r
L-valine + H2O + NAD+	-	Lysinibacillus sphaericus	3-methyl-2-oxobutanoate + NH3 + NADH	-	r

Subunits

Subunits	Comment	Organism
decamer	10 * 39000, SDS_PAGE	Lysinibacillus sphaericus

Synonyms

Synonyms	Comment	Organism
PheDH	-	Lysinibacillus sphaericus

Cofactor

Cofactor	Comment	Organism	Structure
NAD+	-	Lysinibacillus sphaericus
NADH	-	Lysinibacillus sphaericus

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Release 2023.1 (January 2023)