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Literature summary for 1.4.1.20 extracted from

  • Brunhuber, N.M.W.; Thoden, J.B.; Blanchard, J.S.; Vanhooke, J.L.
    Rhodococcus L-Phenylalanine Dehydrogenase: Kinetics, Mechanism, and Structural Basis for Catalytic Specificity (2000), Biochemistry, 39, 9174-9187.
    View publication on PubMed
Search for more literature for 1.4.1.20

Inhibitors

Inhibitors Comment Organism Structure
D-Phe
-
 Rhodococcus sp.
L-Phe
-
 Rhodococcus sp.
L-Phenyllactate
-
 Rhodococcus sp.
NADH
-
 Rhodococcus sp.
NH4+
-
 Rhodococcus sp.
Phenylethylamine
-
 Rhodococcus sp.
phenylpropionate
-
 Rhodococcus sp.
phenylpyruvate
-
 Rhodococcus sp.
trans-cinnamate
-
 Rhodococcus sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
40
-
 oxidized beta-nicotinamide hypoxanthine dinucleotide
-
 Rhodococcus sp.
50
-
 3-pyridinealdehyde-NAD+
-
 Rhodococcus sp.
60
-
 thioNAD+
-
 Rhodococcus sp.
90
-
 NAD+
-
 Rhodococcus sp.
110
-
 3-acetylpyridine-NAD+
-
 Rhodococcus sp.
170
-
 oxidized beta-nicotinamide guanine dinucleotide
-
 Rhodococcus sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
39500
-
 4 * 39500, SDS-PAGE Rhodococcus sp.
150000
-
 gel filtration Rhodococcus sp.

Organism

Organism UniProt Comment Textmining
Rhodococcus sp.
-
-
-

Reaction

Reaction Comment Organism Reaction ID
L-phenylalanine + H2O + NAD+ = phenylpyruvate + NH3 + NADH + H+ kinetic mechanism is ordered with NAD+ binding prior to Phe and the products being released in the order of ammonia, phenylpyruvate, and NADH Rhodococcus sp.
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-Phe + H2O + NAD+
-
 Rhodococcus sp. phenylpyruvate + NH3 + NADH
-
 r
L-phenylalanine + H2O + 3-acetylpyridine-NAD+ 350% of the activity with NAD+ Rhodococcus sp. phenylpyruvate + NH3 + acetylpyridine-NADH
-
 ?
L-phenylalanine + H2O + 3-pyridinealdehyde-NAD+ 55% of the activity with NAD+ Rhodococcus sp. phenylpyruvate + NH3 + 3-pyridinealdehyde-NADH
-
 ?
L-phenylalanine + H2O + oxidized beta-nicotinamide guanine dinucleotide at 86% of the activity with NAD+ Rhodococcus sp. phenylpyruvate + NH3 + reduced beta-nicotinamide guanine dinucleotide
-
 ?
L-phenylalanine + H2O + oxidized beta-nicotinamide hypoxanthine dinucleotide at 86% of the activity with NAD+ Rhodococcus sp. phenylpyruvate + NH3 + reduced beta-nicotinamide hypoxanthine dinucleotide
-
 ?
L-phenylalanine + H2O + thio-NAD+ at 86% of the activity with NAD+ Rhodococcus sp. phenylpyruvate + NH3 + thio-NADH
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 39500, SDS-PAGE Rhodococcus sp.

Cofactor

Cofactor Comment Organism Structure
additional information no activity with NADH Rhodococcus sp.
NAD+
-
 Rhodococcus sp.
NADH
-
 Rhodococcus sp.