Literature summary for 1.4.1.20 extracted from

Cooper, A.J.L.; Leung, L.K.H.; Asano, Y.
Enzymatic cycling assay for phenylpyruvate (1989), Anal. Biochem., 183, 210-214.

Search for more literature for 1.4.1.20

Application

Application	Comment	Organism
analysis	enzymatic cycling assays for the determination of L-Phe and phenylpyruvate. Assay 1 couples glutamine transferase K with L-phenylalanine dehydrogenase. Assay 2 combines phenylanine dehydrogenase, L-amino acid oxidase, and catalase	Sporosarcina ureae
analysis	enzymatic cycling assays for the determination of L-Phe and phenylpyruvate. Assay 1 couples glutamine transferase K with L-phenylalanine dehydrogenase. Assay 2 combines phenylanine dehydrogenase, L-amino acid oxidase, and catalase	Bacillus badius

Organism

Organism	UniProt	Comment	Textmining
Bacillus badius	-	-	-
Sporosarcina ureae	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
L-Phe + H2O + NAD+	-	Sporosarcina ureae	phenylpyruvate + NH3 + NADH	-	r
L-Phe + H2O + NAD+	-	Bacillus badius	phenylpyruvate + NH3 + NADH	-	r

Cofactor

Cofactor	Comment	Organism	Structure
NAD+	-	Sporosarcina ureae
NAD+	-	Bacillus badius
NADH	-	Sporosarcina ureae
NADH	-	Bacillus badius

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Release 2023.1 (January 2023)