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Literature summary for 1.4.1.20 extracted from

  • Wendel, U.; Hummel, W.; Langenbeck, U.
    Monitoring of phenylketonuria: a colorimetric method for the determination of plasma phenylalanine using L-phenylalanine dehydrogenase (1989), Anal. Biochem., 180, 91-94.
    View publication on PubMed
Search for more literature for 1.4.1.20

Application

Application Comment Organism
analysis monitoring of phenylketonuria, colorimetric method for the determination of plasma phenylalanine using L-phenylalanine dehydrogenase Rhodococcus sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.75
-
 L-Phe
-
 Rhodococcus sp.
3.1
-
 L-Tyr
-
 Rhodococcus sp.

Organism

Organism UniProt Comment Textmining
Rhodococcus sp.
-
 M4
-
Rhodococcus sp. M4
-
 M4
-

Source Tissue

Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-Phe + H2O + NAD+ the equilibrium favors L-Phe formation Rhodococcus sp. phenylpyruvate + NH3 + NADH
-
 r
L-Phe + H2O + NAD+ the equilibrium favors L-Phe formation Rhodococcus sp. M4 phenylpyruvate + NH3 + NADH
-
 r
L-Tyr + H2O + NAD+
-
 Rhodococcus sp. 4-hydroxyphenylpyruvate + NH3 + NADH
-
 ?
L-Tyr + H2O + NAD+
-
 Rhodococcus sp. M4 4-hydroxyphenylpyruvate + NH3 + NADH
-
 ?

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Rhodococcus sp.
NADH
-
 Rhodococcus sp.