Application | Comment | Organism |
---|---|---|
synthesis | continous production of L-Phe in an enzyme-membrane-reactor | Rhodococcus sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
L-Phe | - |
Rhodococcus sp. | |
NAD+ | - |
Rhodococcus sp. | |
NADH | - |
Rhodococcus sp. | |
phenylpyruvate | - |
Rhodococcus sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.13 | - |
NADH | - |
Rhodococcus sp. | |
0.16 | - |
phenylpyruvate | - |
Rhodococcus sp. | |
0.27 | - |
NAD+ | - |
Rhodococcus sp. | |
0.43 | - |
L-Met | - |
Rhodococcus sp. | |
0.75 | - |
L-Phe | - |
Rhodococcus sp. | |
2.1 | - |
2-oxo-4-methylthiobutanoate | - |
Rhodococcus sp. | |
2.4 | - |
p-hydroxyphenylpyruvate | - |
Rhodococcus sp. | |
3.1 | - |
L-Tyr | - |
Rhodococcus sp. | |
7.7 | - |
Indolepyruvate | - |
Rhodococcus sp. | |
11 | - |
L-Trp | - |
Rhodococcus sp. | |
387 | - |
NH4+ | - |
Rhodococcus sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodococcus sp. | - |
M4 | - |
Rhodococcus sp. M4 | - |
M4 | - |
Purification (Comment) | Organism |
---|---|
- |
Rhodococcus sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxo-4-methylthiobutanoate + NH3 + NADH | 33% of the activity with L-Phe | Rhodococcus sp. | L-Met + NAD+ + H2O | - |
? | |
4-hydroxyphenylpyruvate + NH3 + NADH | 5% of the activity with L-Phe | Rhodococcus sp. | L-Tyr + NAD+ + H2O | - |
? | |
4-hydroxyphenylpyruvate + NH3 + NADH | 5% of the activity with L-Phe | Rhodococcus sp. M4 | L-Tyr + NAD+ + H2O | - |
? | |
indolepyruvate + NH3 + NADH | 3% of the activity with L-Phe | Rhodococcus sp. | ? | - |
? | |
L-Met + H2O + NAD+ | 4% of the activity with L-Phe | Rhodococcus sp. | 2-oxo-4-methylthiobutanoate + NH3 + NADH | - |
? | |
L-Met + H2O + NAD+ | 4% of the activity with L-Phe | Rhodococcus sp. M4 | 2-oxo-4-methylthiobutanoate + NH3 + NADH | - |
? | |
L-Phe + H2O + NAD+ | - |
Rhodococcus sp. | phenylpyruvate + NH3 + NADH | - |
r | |
L-Phe + H2O + NAD+ | - |
Rhodococcus sp. M4 | phenylpyruvate + NH3 + NADH | - |
r | |
L-Trp + H2O + NAD+ | 2% of the activity with L-Phe | Rhodococcus sp. | indole-3-pyruvate + NH3 + NADH | - |
? | |
L-Trp + H2O + NAD+ | 2% of the activity with L-Phe | Rhodococcus sp. M4 | indole-3-pyruvate + NH3 + NADH | - |
? | |
L-Tyr + H2O + NAD+ | - |
Rhodococcus sp. | 4-hydroxyphenylpyruvate + NH3 + NADH | - |
? | |
L-Tyr + H2O + NAD+ | - |
Rhodococcus sp. M4 | 4-hydroxyphenylpyruvate + NH3 + NADH | - |
? |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 70 | 20°C: about 50% of maximal activity, 70°C: about 70% of maximal activity | Rhodococcus sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9.3 | - |
reductive amination | Rhodococcus sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Rhodococcus sp. | |
NADH | - |
Rhodococcus sp. |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.002 | - |
NADH | - |
Rhodococcus sp. | |
0.07 | - |
phenylpyruvate | - |
Rhodococcus sp. | |
1.27 | - |
NAD+ | - |
Rhodococcus sp. | |
7.34 | - |
phenylpyruvate | - |
Rhodococcus sp. | |
17.85 | - |
Phe | - |
Rhodococcus sp. |