Activating Compound | Comment | Organism | Structure |
---|---|---|---|
D-arginine | 212% activity in the presence of 10 mM D-arginine | Janthinobacterium lividum | |
L-arginine | 936% activity in the presence of 10 mM L-arginine | Janthinobacterium lividum | |
L-asparagine | 118% activity in the presence of 10 mM L-asparagine | Janthinobacterium lividum | |
L-aspartate | 1735% activity in the presence of 10 mM L-aspartate | Janthinobacterium lividum | |
L-histidine | 155% activity in the presence of 10 mM L-histidine | Janthinobacterium lividum | |
L-lysine | 161% activity in the presence of 10 mM L-lysine | Janthinobacterium lividum | |
L-methionine | 206% activity in the presence of 10 mM L-methionine | Janthinobacterium lividum | |
L-tryptophan | 235% activity in the presence of 10 mM L-tryptophan | Janthinobacterium lividum | |
Zn2+ | 224% activity in the presence of 1 mM Zn2+ | Janthinobacterium lividum |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli Top10 cells | Janthinobacterium lividum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
citrate | 67% activity in the presence of 10 mM citrate | Janthinobacterium lividum | |
Co2+ | 82% activity in the presence of 1 mM Co2+ | Janthinobacterium lividum | |
D-asparagine | 93% activity in the presence of 10 mM D-asparagine | Janthinobacterium lividum | |
D-Aspartate | 90% activity in the presence of 10 mM D-aspartate | Janthinobacterium lividum | |
D-glutamate | 94% activity in the presence of 10 mM D-glutamate | Janthinobacterium lividum | |
D-glutamine | 95% activity in the presence of 10 mM D-glutamine | Janthinobacterium lividum | |
fumarate | 32% activity in the presence of 10 mM fumarate | Janthinobacterium lividum | |
Hg2+ | no activity in the presence of 1 mM Hg2+ | Janthinobacterium lividum | |
isocitrate | 49% activity in the presence of 10 mM isocitrate | Janthinobacterium lividum | |
L-glutamate | substrate inhibition at L-glutamate concentrations above 20 mM | Janthinobacterium lividum | |
L-glutamine | 89% activity in the presence of 10 mM L-glutamine | Janthinobacterium lividum | |
L-ornithine | 83% activity in the presence of 10 mM L-ornithine | Janthinobacterium lividum | |
malate | 65% activity in the presence of 10 mM malate | Janthinobacterium lividum | |
Mg2+ | 64% activity in the presence of 1 mM Mg2+ | Janthinobacterium lividum | |
Mn2+ | 52% activity in the presence of 1 mM Mn2+ | Janthinobacterium lividum | |
additional information | not inhibited by NAD+ | Janthinobacterium lividum | |
Ni2+ | 77% activity in the presence of 1 mM Ni2+ | Janthinobacterium lividum | |
succinate | 36% activity in the presence of 10 mM succinate | Janthinobacterium lividum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.1 | - |
NAD+ | in the presence of 20 mM L-glutamate, in 100 mM glycine/NaOH (pH 9.5), at 25°C | Janthinobacterium lividum | |
7.1 | - |
L-glutamate | in the presence of 5 mM NAD+, in 100 mM glycine/NaOH (pH 9.5), at 25°C | Janthinobacterium lividum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
169360 | - |
6 * 169360, deduced from amino acid sequence | Janthinobacterium lividum |
170000 | - |
6 * 170000, SDS-PAGE | Janthinobacterium lividum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Janthinobacterium lividum | A5LH94 | fragment | - |
Janthinobacterium lividum UTB1302 | A5LH94 | fragment | - |
Purification (Comment) | Organism |
---|---|
DEAE-Toyopearl column chromatography, butyl-Toyopearl column chromatography, and Superdex 200 gel filtration | Janthinobacterium lividum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.62 | - |
recombinant enzyme from crude cell extract, at 25°C | Janthinobacterium lividum |
3.1 | - |
purified recombinant enzyme, at 25°C | Janthinobacterium lividum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamate + H2O + NAD+ | - |
Janthinobacterium lividum | 2-oxoglutarate + NH3 + NADH | - |
? | |
L-glutamate + H2O + NAD+ | - |
Janthinobacterium lividum UTB1302 | 2-oxoglutarate + NH3 + NADH | - |
? | |
additional information | inert reaction with L-glutamine, L-aspartate, L-alanine, L-leucine, L-valine, L-lysine, L-2-aminobutyrate, L-methionine, L-ornithine, L-phenylalanine, L-arginine, L-tryptophan, L-methionine, L-histidine, D-glutamate, and D-aspartate, the enzyme is not active with NADP+ | Janthinobacterium lividum | ? | - |
? | |
additional information | inert reaction with L-glutamine, L-aspartate, L-alanine, L-leucine, L-valine, L-lysine, L-2-aminobutyrate, L-methionine, L-ornithine, L-phenylalanine, L-arginine, L-tryptophan, L-methionine, L-histidine, D-glutamate, and D-aspartate, the enzyme is not active with NADP+ | Janthinobacterium lividum UTB1302 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homohexamer | 6 * 169360, deduced from amino acid sequence | Janthinobacterium lividum |
homohexamer | 6 * 170000, SDS-PAGE | Janthinobacterium lividum |
Synonyms | Comment | Organism |
---|---|---|
NAD-dependent L-glutamate dehydrogenase | - |
Janthinobacterium lividum |
NAD-GDH | - |
Janthinobacterium lividum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
in glycine-NaOH buffer at pH 9.0 | Janthinobacterium lividum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 50 | the enzyme is stable for 10 min at temperatures up to 40°C but is completely inactivated by incubation for 10 min at 50°C | Janthinobacterium lividum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
in glycine-NaOH buffer at 40°C | Janthinobacterium lividum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | dependent | Janthinobacterium lividum |