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Literature summary for 1.4.1.2 extracted from

  • Camardella, L.; di Fraia, R.; Antignani, A.; Ciardiello, M.A.; di Prisco, G.; Coleman, J.K.; Buchon, L.; Guespin, J.; Russell, N.J.
    The antarctic Psychrobacter sp. TAD1 has two cold-active glutamate dehydrogenases with different cofactor specificities. Characterisation of the NAD+-dependent enzyme (2002), Comp. Biochem. Physiol. A, 131A, 559-567.
No PubMed abstract available
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KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.5
-
 NAD+ pH 8.0, 20°C Psychrobacter sp.
2.36
-
 2-oxoglutarate pH 8.0, 20°C Psychrobacter sp.
24.6
-
 NH3 pH 8.0, 20°C Psychrobacter sp.
28.6
-
 L-glutamate pH 8.0, 20°C Psychrobacter sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
160000
-
 2 * 160000, SDS-PAGE Psychrobacter sp.
300000
-
 gel filtration Psychrobacter sp.

Organism

Organism UniProt Comment Textmining
Psychrobacter sp.
-
-
-
Psychrobacter sp. TAD1
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Psychrobacter sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
248
-
-
 Psychrobacter sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-oxoglutarate + NADH + NH3
-
 Psychrobacter sp. L-glutamate + NAD+ + H2O
-
 r
2-oxoglutarate + NADH + NH3
-
 Psychrobacter sp. TAD1 L-glutamate + NAD+ + H2O
-
 r

Subunits

Subunits Comment Organism
dimer 2 * 160000, SDS-PAGE Psychrobacter sp.

Synonyms

Synonyms Comment Organism
NAD+-dependent GDH
-
 Psychrobacter sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
20
-
-
 Psychrobacter sp.

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
10 43 10°C: about 45% of maximal activity, 43°C: about 75% of maximal activity Psychrobacter sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
 about 45% loss of activity after 15 min, about 60% loss of activity after 30 min Psychrobacter sp.
40
-
 about 60% loss of activity after 5 min, about 80% loss of activity after 15 min Psychrobacter sp.

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Psychrobacter sp.
NADH
-
 Psychrobacter sp.