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Literature summary for 1.4.1.2 extracted from

  • Jahns, T.
    Unusually stable NAD-specific glutamate dehydrogenase from the alkaliphile Amphibacillus xylanus (1996), Antonie van Leeuwenhoek, 70, 89-95.
    View publication on PubMed

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
6 * 45000, SDS-PAGE Amphibacillus xylanus
260000
-
gel filtration Amphibacillus xylanus

Organism

Organism UniProt Comment Textmining
Amphibacillus xylanus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Amphibacillus xylanus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
822
-
-
Amphibacillus xylanus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-oxoglutarate + NH3 + NADH
-
Amphibacillus xylanus L-glutamate + H2O + NAD+
-
r
L-glutamate + H2O + NAD+
-
Amphibacillus xylanus 2-oxoglutarate + NH3 + NADH + H+
-
r

Subunits

Subunits Comment Organism
hexamer 6 * 45000, SDS-PAGE Amphibacillus xylanus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
85
-
120 min, 50% activity loss Amphibacillus xylanus

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Amphibacillus xylanus