Literature summary for 1.4.1.2 extracted from

Veronese, F.M.; Nyc, J.F.; Degani, Y.; Brown, D.M.; Smith, E.L.
Nicotinamide adenine dinucleotide-specific glutamate dehydrogenase of Neurospora. I. Purification and molecular properties (1974), J. Biol. Chem., 249, 7922-7928.

Search for more literature for 1.4.1.2

General Stability

General Stability	Organism
DTT stabilizes	Neurospora crassa
isophthalate stabilizes	Neurospora crassa

Inhibitors

Inhibitors	Comment	Organism	Structure
isophthalate	-	Neurospora crassa

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
116000	-	4 * 116000, sedimentation equilibrium analysis after treatment with 6 M guanidine HCl and 0.5% mercaptoethanol	Neurospora crassa
480000	-	sedimentation equilibrium analysis	Neurospora crassa

Organism

Organism	UniProt	Comment	Textmining
Neurospora crassa	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Neurospora crassa

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
additional information	-	-	Neurospora crassa

Storage Stability

Storage Stability	Organism
-20°C, gradual loss of activity	Neurospora crassa
4°C, suspension of 50% saturated ammonium sulfate, 0.1 mM DTT, 25 mM glutamate, stable for months	Neurospora crassa

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
L-glutamate + H2O + NAD+	-	Neurospora crassa	2-oxoglutarate + NH3 + NADH + H+	-	?

Subunits

Subunits	Comment	Organism
tetramer	4 * 116000, sedimentation equilibrium analysis after treatment with 6 M guanidine HCl and 0.5% mercaptoethanol	Neurospora crassa

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
50	-	6 min, complete loss of activity	Neurospora crassa

Cofactor

Cofactor	Comment	Organism	Structure
NAD+	-	Neurospora crassa
NADH	-	Neurospora crassa

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)