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Literature summary for 1.4.1.2 extracted from
- Steady-state kinetics of glutamate dehydrogenase from Pisum sativum L. mitochondria (1977), Arch. Biochem. Biophys., 182, 614-625.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| alpha-ketoglutarate | - |
Pisum sativum |  |
| Ca2+ | activation of reductive amination; slight inhibition of oxidative deamination | Pisum sativum | |
| NAD+ | - |
Pisum sativum | |
| NADH | - |
Pisum sativum | |
| NH4+ | - |
Pisum sativum | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| mitochondrion | - |
Pisum sativum | 5739 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | activation of reductive amination | Pisum sativum | |
| Ca2+ | slight inhibition of oxidative deamination | Pisum sativum | |
| Mn2+ | activation of reductive amination | Pisum sativum | |
| Zn2+ | activation of reductive amination | Pisum sativum | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pisum sativum | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Pisum sativum |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| L-glutamate + H2O + NAD+ = 2-oxoglutarate + NH3 + NADH + H+ | partially random mechanism | Pisum sativum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| stem | - |
Pisum sativum | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 54 | - |
- |
Pisum sativum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-glutamate + H2O + NAD+ | - |
Pisum sativum | 2-oxoglutarate + NH3 + NADH + H+ | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
reductive amination | Pisum sativum |
| 9.2 | - |
oxidative deamination | Pisum sativum |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 8.8 | about 50% of activity maximum at pH 7.0 and 8.8, reductive amination | Pisum sativum |
| 8.4 | 10.1 | 50% of activity maximum at pH 8.4 and 10.1, oxidative deamination | Pisum sativum |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Pisum sativum | |
| NADH | - |
Pisum sativum | |
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Release 2023.1 (January 2023)
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