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Literature summary for 1.4.1.19 extracted from

  • Ogura, R.; Wakamatsu, T.; Mutaguchi, Y.; Doi, K.; Ohshima, T.
    Biochemical characterization of an L-tryptophan dehydrogenase from the photoautotrophic cyanobacterium Nostoc punctiforme (2014), Enzyme Microb. Technol., 60, 40-46 .
    View publication on PubMed
Search for more literature for 1.4.1.19

Activating Compound

Activating Compound Comment Organism Structure
additional information not influenced by EDTA (10 mM), KCl (100 mM) or DMSO (1% (v/v)) Nostoc punctiforme

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Nostoc punctiforme

Inhibitors

Inhibitors Comment Organism Structure
(indol-3-yl)pyruvate substrate inhibition Nostoc punctiforme

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.014
-
 (indol-3-yl)pyruvate at pH 7.5 and 25°C Nostoc punctiforme
0.025
-
 NAD+ at pH 11.0 and 25°C Nostoc punctiforme
0.031
-
 L-tryptophan at pH 11.0 and 25°C Nostoc punctiforme
0.038
-
 NADH at pH 7.5 and 25°C Nostoc punctiforme
130
-
 NH3 at pH 7.5 and 25°C Nostoc punctiforme

Metals/Ions

Metals/Ions Comment Organism Structure
additional information the enzyme is not activated by metal ions Nostoc punctiforme

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
76100
-
 gel filtration Nostoc punctiforme

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(indol-3-yl)pyruvate + NH3 + NADH + H+ Nostoc punctiforme
-
 L-tryptophan + NAD+ + H2O
-
 r
(indol-3-yl)pyruvate + NH3 + NADH + H+ Nostoc punctiforme NIES-2108
-
 L-tryptophan + NAD+ + H2O
-
 r
L-tryptophan + NAD+ + H2O Nostoc punctiforme the enzyme is highly specific for the deamination of amino acid L-tryptophan (indol-3-yl)pyruvate + NH3 + NADH + H+
-
 r
L-tryptophan + NAD+ + H2O Nostoc punctiforme NIES-2108 the enzyme is highly specific for the deamination of amino acid L-tryptophan (indol-3-yl)pyruvate + NH3 + NADH + H+
-
 r

Organism

Organism UniProt Comment Textmining
Nostoc punctiforme
-
-
-
Nostoc punctiforme NIES-2108
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA agarose column chromatography Nostoc punctiforme

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(indol-3-yl)pyruvate + NH3 + NADH + H+
-
 Nostoc punctiforme L-tryptophan + NAD+ + H2O
-
 r
(indol-3-yl)pyruvate + NH3 + NADH + H+
-
 Nostoc punctiforme NIES-2108 L-tryptophan + NAD+ + H2O
-
 r
L-phenylalanine + NAD+ + H2O 23% activity compared to L-tryptophan Nostoc punctiforme phenylpyruvate + NH3 + NADH + H+
-
 ?
L-phenylalanine + NAD+ + H2O 23% activity compared to L-tryptophan Nostoc punctiforme NIES-2108 phenylpyruvate + NH3 + NADH + H+
-
 ?
L-tryptophan + NAD+ + H2O the enzyme is highly specific for the deamination of amino acid L-tryptophan Nostoc punctiforme (indol-3-yl)pyruvate + NH3 + NADH + H+
-
 r
L-tryptophan + NAD+ + H2O the enzyme is highly specific for the deamination of amino acid L-tryptophan Nostoc punctiforme NIES-2108 (indol-3-yl)pyruvate + NH3 + NADH + H+
-
 r
additional information the enzyme shows no activity with D-tryptophan, L-tyrosine, L-alanine, L-valine, L-leucine, L-isoleucine, L-proline, L-cystine, L-methionine, L-asparagine, L-glutamine, L-aspartic acid, L-glutamic acid, L-serine, L-threonine, L-histine, L-arginine, L-lysine, glcyine, phenylpyruvate, NADP+, and NADPH Nostoc punctiforme ?
-
 ?
additional information the enzyme shows no activity with D-tryptophan, L-tyrosine, L-alanine, L-valine, L-leucine, L-isoleucine, L-proline, L-cystine, L-methionine, L-asparagine, L-glutamine, L-aspartic acid, L-glutamic acid, L-serine, L-threonine, L-histine, L-arginine, L-lysine, glcyine, phenylpyruvate, NADP+, and NADPH Nostoc punctiforme NIES-2108 ?
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 39500, calculated from amino acid sequence Nostoc punctiforme

Synonyms

Synonyms Comment Organism
NAD+-dependent L-tryptophan dehydrogenase
-
 Nostoc punctiforme
TrpDH
-
 Nostoc punctiforme

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
 Nostoc punctiforme

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
35 55 more than 50% activity between 35 and 55°C Nostoc punctiforme

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40 50 the enzyme retains full activity after incubation for 10 min at temperatures up to 40°C. There is a complete loss of activity when the enzyme is incubated at temperatures above 50°C Nostoc punctiforme

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.2
-
 L-tryptophan at pH 11.0 and 25°C Nostoc punctiforme
4.3
-
 NAD+ at pH 11.0 and 25°C Nostoc punctiforme
16
-
 (indol-3-yl)pyruvate at pH 7.5 and 25°C Nostoc punctiforme
17
-
 NH3 at pH 7.5 and 25°C Nostoc punctiforme
21
-
 NADH at pH 7.5 and 25°C Nostoc punctiforme

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 for amination of 3-indolpyruvate Nostoc punctiforme
11
-
 for deamination of L-tryptophan Nostoc punctiforme

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 11.5 the enzyme retains more than 80% of its activity after incubation for 30 min at pH 5.0 to 11.5 Nostoc punctiforme

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Nostoc punctiforme
NADH
-
 Nostoc punctiforme

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.014
-
 (indol-3-yl)pyruvate at pH 11.0 and 25°C Nostoc punctiforme

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.13
-
 NH3 at pH 7.5 and 25°C Nostoc punctiforme
140
-
 L-tryptophan at pH 11.0 and 25°C Nostoc punctiforme
170
-
 NAD+ at pH 11.0 and 25°C Nostoc punctiforme
550
-
 NADH at pH 7.5 and 25°C Nostoc punctiforme
1100
-
 (indol-3-yl)pyruvate at pH 7.5 and 25°C Nostoc punctiforme