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Literature summary for 1.4.1.19 extracted from

  • Wakamatsu, T.; Sakuraba, H.; Kitamura, M.; Hakumai, Y.; Fukui, K.; Ohnishi, K.; Ashiuchi, M.; Ohshima, T.
    Structural insights into l-tryptophan dehydrogenase from a photoautotrophic Cyanobacterium, Nostoc punctiforme (2017), Appl. Environ. Microbiol., 83, e02710 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.4.1.19

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Rosetta-gami2(DE3)pLysS cells Nostoc punctiforme

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method, using 0.1 M HEPES-NaOH (pH 7.5), 14% (w/v) polyethylene glycol 8000, and 8% (v/v) ethylene glycol Nostoc punctiforme

Protein Variants

Protein Variants Comment Organism
A69L the mutant shows severely reduced catalytic efficiency compared to the wild type enzyme Nostoc punctiforme
A69M the mutant shows severely reduced catalytic efficiency compared to the wild type enzyme Nostoc punctiforme
I296A the mutant shows reduced catalytic efficiency compared to the wild type enzyme Nostoc punctiforme
L288M the mutant shows severely reduced catalytic efficiency compared to the wild type enzyme Nostoc punctiforme
L59F/D168G/A234D/I296N the mutant exhibits higher stability and specific activity than the wild type enzyme Nostoc punctiforme
M295A the mutant shows reduced catalytic efficiency compared to the wild type enzyme Nostoc punctiforme
M40L the mutant shows reduced catalytic efficiency compared to the wild type enzyme Nostoc punctiforme
M65A the mutant shows severely reduced catalytic efficiency compared to the wild type enzyme Nostoc punctiforme
V132A the mutant shows reduced catalytic efficiency compared to the wild type enzyme Nostoc punctiforme
V133A the mutant shows reduced catalytic efficiency compared to the wild type enzyme Nostoc punctiforme
V291A the mutant shows reduced catalytic efficiency compared to the wild type enzyme Nostoc punctiforme
Y292F the mutant shows increased catalytic efficiency compared to the wild type enzyme Nostoc punctiforme
Y292H the mutant shows reduced catalytic efficiency compared to the wild type enzyme Nostoc punctiforme
Y292W the mutant shows reduced catalytic efficiency compared to the wild type enzyme Nostoc punctiforme

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.017
-
 L-tryptophan mutant enzyme V132A, at pH 11.0 and 25°C Nostoc punctiforme
0.019
-
 L-tryptophan mutant enzyme V133A, at pH 11.0 and 25°C Nostoc punctiforme
0.021
-
 L-tryptophan mutant enzyme Y292H, at pH 11.0 and 25°C Nostoc punctiforme
0.03
-
 L-tryptophan mutant enzyme Y292W, at pH 11.0 and 25°C Nostoc punctiforme
0.039
-
 L-tryptophan mutant enzyme Y292F, at pH 11.0 and 25°C Nostoc punctiforme
0.045
-
 L-tryptophan wild type enzyme, at pH 11.0 and 25°C Nostoc punctiforme
0.047
-
 L-tryptophan mutant enzyme M295A, at pH 11.0 and 25°C Nostoc punctiforme
0.047
-
 L-tryptophan mutant enzyme M40L, at pH 11.0 and 25°C Nostoc punctiforme
0.047
-
 L-tryptophan mutant enzyme V291A, at pH 11.0 and 25°C Nostoc punctiforme
0.059
-
 L-tryptophan mutant enzyme I296A, at pH 11.0 and 25°C Nostoc punctiforme
0.2
-
 L-tryptophan mutant enzyme M65A, at pH 11.0 and 25°C Nostoc punctiforme
0.52
-
 L-tryptophan mutant enzyme L288M, at pH 11.0 and 25°C Nostoc punctiforme
0.82
-
 L-tryptophan mutant enzyme A69M, at pH 11.0 and 25°C Nostoc punctiforme
1.1
-
 L-tryptophan mutant enzyme A69L, at pH 11.0 and 25°C Nostoc punctiforme

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-tryptophan + NAD+ + H2O Nostoc punctiforme
-
 (indol-3-yl)pyruvate + NH3 + NADH + H+
-
 r
L-tryptophan + NAD+ + H2O Nostoc punctiforme NIES-2108
-
 (indol-3-yl)pyruvate + NH3 + NADH + H+
-
 r

Organism

Organism UniProt Comment Textmining
Nostoc punctiforme W8CV45
-
-
Nostoc punctiforme NIES-2108 W8CV45
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Nostoc punctiforme

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-tryptophan + NAD+ + H2O
-
 Nostoc punctiforme (indol-3-yl)pyruvate + NH3 + NADH + H+
-
 r
L-tryptophan + NAD+ + H2O
-
 Nostoc punctiforme NIES-2108 (indol-3-yl)pyruvate + NH3 + NADH + H+
-
 r

Subunits

Subunits Comment Organism
homodimer 2 * 40000, SDS-PAGE Nostoc punctiforme

Synonyms

Synonyms Comment Organism
L-Trp dehydrogenase
-
 Nostoc punctiforme
TrpDH
-
 Nostoc punctiforme

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.39
-
 L-tryptophan mutant enzyme L288M, at pH 11.0 and 25°C Nostoc punctiforme
1.1
-
 L-tryptophan mutant enzyme M65A, at pH 11.0 and 25°C Nostoc punctiforme
1.2
-
 L-tryptophan mutant enzyme A69L, at pH 11.0 and 25°C Nostoc punctiforme
1.2
-
 L-tryptophan mutant enzyme A69M, at pH 11.0 and 25°C Nostoc punctiforme
2.6
-
 L-tryptophan mutant enzyme V133A, at pH 11.0 and 25°C Nostoc punctiforme
2.9
-
 L-tryptophan mutant enzyme V291A, at pH 11.0 and 25°C Nostoc punctiforme
3.5
-
 L-tryptophan mutant enzyme Y292H, at pH 11.0 and 25°C Nostoc punctiforme
4.6
-
 L-tryptophan mutant enzyme V132A, at pH 11.0 and 25°C Nostoc punctiforme
8
-
 L-tryptophan mutant enzyme M295A, at pH 11.0 and 25°C Nostoc punctiforme
8.5
-
 L-tryptophan mutant enzyme Y292W, at pH 11.0 and 25°C Nostoc punctiforme
10
-
 L-tryptophan mutant enzyme I296A, at pH 11.0 and 25°C Nostoc punctiforme
12
-
 L-tryptophan mutant enzyme M40L, at pH 11.0 and 25°C Nostoc punctiforme
20
-
 L-tryptophan wild type enzyme, at pH 11.0 and 25°C Nostoc punctiforme
21
-
 L-tryptophan mutant enzyme Y292F, at pH 11.0 and 25°C Nostoc punctiforme

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Nostoc punctiforme

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.75
-
 L-tryptophan mutant enzyme L288M, at pH 11.0 and 25°C Nostoc punctiforme
1.1
-
 L-tryptophan mutant enzyme A69L, at pH 11.0 and 25°C Nostoc punctiforme
1.5
-
 L-tryptophan mutant enzyme A69M, at pH 11.0 and 25°C Nostoc punctiforme
5.5
-
 L-tryptophan mutant enzyme M65A, at pH 11.0 and 25°C Nostoc punctiforme
62
-
 L-tryptophan mutant enzyme V291A, at pH 11.0 and 25°C Nostoc punctiforme
140
-
 L-tryptophan mutant enzyme V133A, at pH 11.0 and 25°C Nostoc punctiforme
170
-
 L-tryptophan mutant enzyme I296A, at pH 11.0 and 25°C Nostoc punctiforme
170
-
 L-tryptophan mutant enzyme M295A, at pH 11.0 and 25°C Nostoc punctiforme
170
-
 L-tryptophan mutant enzyme Y292H, at pH 11.0 and 25°C Nostoc punctiforme
260
-
 L-tryptophan mutant enzyme M40L, at pH 11.0 and 25°C Nostoc punctiforme
270
-
 L-tryptophan mutant enzyme V132A, at pH 11.0 and 25°C Nostoc punctiforme
280
-
 L-tryptophan mutant enzyme Y292W, at pH 11.0 and 25°C Nostoc punctiforme
440
-
 L-tryptophan wild type enzyme, at pH 11.0 and 25°C Nostoc punctiforme
540
-
 L-tryptophan mutant enzyme Y292F, at pH 11.0 and 25°C Nostoc punctiforme