KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.36 | - |
NADH | tumor C-58 cell culture | Nicotiana tabacum | |
1.39 | - |
NADH | tumor T-24 cell culture | Nicotiana tabacum | |
1.46 | - |
NADH | normal cell culture | Nicotiana tabacum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-tryptophan + NAD(P)+ | Nicotiana tabacum | primary enzyme of indolylpyruvate pathway leading to indol-3-yl-acetic acid in plants, metabolism of indole compounds in plants | indol-3-yl-pyruvate + NH3 + NAD(P)H | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Nicotiana tabacum | - |
normal and crown-gall tumor tissue cultures, tissue transformed by Agrobacterium tumefaciens | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
tissue culture | normal and crown-gall tumor tissue cultures, tissue transformed by Agrobacterium tumefaciens | Nicotiana tabacum | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.87 | - |
cofactor NADH, activity in normal BV-N strain | Nicotiana tabacum |
1.22 | - |
cofactor NADPH, activity in normal BV-N strain | Nicotiana tabacum |
1.24 | - |
cofactor NADH or NADPH, activity in tumor tobacco tissue strains T-24 | Nicotiana tabacum |
1.57 | - |
cofactor NADH, activity in tumor tobacco tissue strain C-58 | Nicotiana tabacum |
1.59 | - |
cofactor NADPH, activity in tumor tobacco tissue strain C-58 | Nicotiana tabacum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(indol-3-yl)pyruvate + NH3 + NADH + H+ | - |
Nicotiana tabacum | L-tryptophan + NAD+ + H2O | - |
r | |
L-tryptophan + NAD(P)+ | - |
Nicotiana tabacum | indol-3-yl-pyruvate + NH3 + NAD(P)H | - |
r | |
L-tryptophan + NAD(P)+ | primary enzyme of indolylpyruvate pathway leading to indol-3-yl-acetic acid in plants, metabolism of indole compounds in plants | Nicotiana tabacum | indol-3-yl-pyruvate + NH3 + NAD(P)H | - |
r |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
assay at room temperature | Nicotiana tabacum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD(P)+ | - |
Nicotiana tabacum | |
NAD(P)H | normal Nicotiana tabacum cells: higher specific activity with NADPH than with NADH, tumor cells of Nicotiana tabacum: no difference in specific activity with NADPH and NADH | Nicotiana tabacum |