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Literature summary for 1.4.1.16 extracted from
- Identification and functional characterization of NAD(P)+ -dependent meso-diaminopimelate dehydrogenase from Numidum massiliense (2020), MicrobiologyOpen, 9, e1059 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Numidum massiliense |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.302 | - |
NADP+ | pH 10.5, temperature not specified in the publication | Numidum massiliense |  |
| 3.34 | - |
meso-2,6-diaminoheptanedioate | pH 10.5, temperature not specified in the publication | Numidum massiliense | |
| 890 | - |
NAD+ | pH 10.5, temperature not specified in the publication | Numidum massiliense | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Numidum massiliense | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant protein | Numidum massiliense |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 701 | - |
pH 10.5, temperature not specified in the publication | Numidum massiliense |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3-methyl-2-oxobutanoate + NH3 + NADPH + H+ | - |
Numidum massiliense | ? | - |
? | |
| 4-methyl-2-oxopentanoic acid + NH3 + NADPH + H+ | - |
Numidum massiliense | ? | - |
? | |
| meso-2,6-diaminoheptanedioate + H2O + NAD+ | - |
Numidum massiliense | L-2-amino-6-oxoheptanedioate + NH3 + NADH + H+ | - |
? | |
| meso-2,6-diaminoheptanedioate + H2O + NADP+ | - |
Numidum massiliense | L-2-amino-6-oxoheptanedioate + NH3 + NADPH + H+ | - |
? | |
| pyruvate + NH3 + NADPH + H+ | - |
Numidum massiliense | D-alanine + NADP+ | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
30 min, complete loss of activity | Numidum massiliense |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 638 | - |
meso-2,6-diaminoheptanedioate | pH 10.5, temperature not specified in the publication | Numidum massiliense | |
| 792 | - |
NADP+ | pH 10.5, temperature not specified in the publication | Numidum massiliense | |
| 8933 | - |
NAD+ | pH 10.5, temperature not specified in the publication | Numidum massiliense | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9 | - |
optimum for reductive amination, 50°C | Numidum massiliense |
| 10.5 | - |
optimum for oxidative deamination, 50°C | Numidum massiliense |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 13 | more than 85% of maximum activity | Numidum massiliense |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | NADP+ is 2.5fold preferred over NAD+ | Numidum massiliense | |
| NADP+ | NADP+ is 2.5fold preferred over NAD+ | Numidum massiliense | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 192 | - |
meso-2,6-diaminoheptanedioate | pH 10.5, temperature not specified in the publication | Numidum massiliense | |
| 1003 | - |
NAD+ | pH 10.5, temperature not specified in the publication | Numidum massiliense | |
| 2617 | - |
NADP+ | pH 10.5, temperature not specified in the publication | Numidum massiliense | |
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