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Literature summary for 1.4.1.16 extracted from
- Insight into the highly conserved and differentiated cofactor-binding sites of meso-diaminopimelate dehydrogenase StDAPDH (2019), J. Chem. Inf. Model., 59, 2331-2338 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Symbiobacterium thermophilum |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| K159R | mutation decreases the kcat/KM value with meso-DAP, the catalytic efficiency toward pyruvic acid increases by 24% | Symbiobacterium thermophilum |
| P69G | mutation results in decrease of kcat/KM with meso-DAP and pyruvic acid | Symbiobacterium thermophilum |
| S90T | mutation results in decrease of kcat/KM with meso-DAP and pyruvic acid | Symbiobacterium thermophilum |
| T70S | catalytic ability of T70S does not change much | Symbiobacterium thermophilum |
| V14L | mutation results in decrease of kcat/KM with meso-DAP and pyruvic acid | Symbiobacterium thermophilum |
| V156D | mutation results in decrease of kcat/KM with meso-DAP and pyruvic acid, almost complete loss of catalytic ability | Symbiobacterium thermophilum |
| V68M | mutation decreases the kcat/KM value with meso-DAP, the catalytic efficiency toward pyruvic acid does not change | Symbiobacterium thermophilum |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.18 | - |
meso-2,6-diaminoheptanedioate | mutant T70S, pH not specified in the publication, temperature not specified in the publication | Symbiobacterium thermophilum |  |
| 0.21 | - |
meso-2,6-diaminoheptanedioate | mutant K159R, pH not specified in the publication, temperature not specified in the publication | Symbiobacterium thermophilum | |
| 0.27 | - |
meso-2,6-diaminoheptanedioate | wild-type, pH not specified in the publication, temperature not specified in the publication | Symbiobacterium thermophilum | |
| 0.27 | - |
meso-2,6-diaminoheptanedioate | mutant S90T, pH not specified in the publication, temperature not specified in the publication | Symbiobacterium thermophilum | |
| 0.43 | - |
meso-2,6-diaminoheptanedioate | mutant P69G, pH not specified in the publication, temperature not specified in the publication | Symbiobacterium thermophilum | |
| 0.52 | - |
meso-2,6-diaminoheptanedioate | mutant V68M, pH not specified in the publication, temperature not specified in the publication | Symbiobacterium thermophilum | |
| 1.02 | - |
meso-2,6-diaminoheptanedioate | mutant V14L, pH not specified in the publication, temperature not specified in the publication | Symbiobacterium thermophilum | |
| 1.37 | - |
meso-2,6-diaminoheptanedioate | mutant V156D, pH not specified in the publication, temperature not specified in the publication | Symbiobacterium thermophilum | |
| 7.33 | - |
pyruvate | mutant V68M, pH not specified in the publication, temperature not specified in the publication | Symbiobacterium thermophilum | |
| 7.58 | - |
pyruvate | mutant K159R, pH not specified in the publication, temperature not specified in the publication | Symbiobacterium thermophilum | |
| 8.86 | - |
pyruvate | wild-type, pH not specified in the publication, temperature not specified in the publication | Symbiobacterium thermophilum | |
| 10.08 | - |
pyruvate | mutant V14L, pH not specified in the publication, temperature not specified in the publication | Symbiobacterium thermophilum | |
| 10.71 | - |
pyruvate | mutant T70S, pH not specified in the publication, temperature not specified in the publication | Symbiobacterium thermophilum | |
| 20.9 | - |
pyruvate | mutant S90T, pH not specified in the publication, temperature not specified in the publication | Symbiobacterium thermophilum | |
| 25.83 | - |
pyruvate | mutant P69G, pH not specified in the publication, temperature not specified in the publication | Symbiobacterium thermophilum | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Symbiobacterium thermophilum | Q67PI3 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| meso-2,6-diaminoheptanedioate + H2O + NADP+ | - |
Symbiobacterium thermophilum | L-2-amino-6-oxoheptanedioate + NH3 + NADPH + H+ | - |
? | |
| additional information | enzyme is able to catalyze reductive amination | Symbiobacterium thermophilum | ? | - |
- |
|
| pyruvate + NH3 + NADPH + H+ | - |
Symbiobacterium thermophilum | D-alanine + NADP+ | - |
? | |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.58 | - |
meso-2,6-diaminoheptanedioate | mutant V156D, pH not specified in the publication, temperature not specified in the publication | Symbiobacterium thermophilum | |
| 1.63 | - |
pyruvate | mutant V14L, pH not specified in the publication, temperature not specified in the publication | Symbiobacterium thermophilum | |
| 4.57 | - |
pyruvate | mutant P69G, pH not specified in the publication, temperature not specified in the publication | Symbiobacterium thermophilum | |
| 6.34 | - |
pyruvate | mutant V68M, pH not specified in the publication, temperature not specified in the publication | Symbiobacterium thermophilum | |
| 7.61 | - |
pyruvate | wild-type, pH not specified in the publication, temperature not specified in the publication | Symbiobacterium thermophilum | |
| 8.07 | - |
pyruvate | mutant K159R, pH not specified in the publication, temperature not specified in the publication | Symbiobacterium thermophilum | |
| 8.3 | - |
pyruvate | mutant T70S, pH not specified in the publication, temperature not specified in the publication | Symbiobacterium thermophilum | |
| 8.81 | - |
meso-2,6-diaminoheptanedioate | mutant S90T, pH not specified in the publication, temperature not specified in the publication | Symbiobacterium thermophilum | |
| 15.52 | - |
pyruvate | mutant S90T, pH not specified in the publication, temperature not specified in the publication | Symbiobacterium thermophilum | |
| 23.86 | - |
meso-2,6-diaminoheptanedioate | mutant T70S, pH not specified in the publication, temperature not specified in the publication | Symbiobacterium thermophilum | |
| 24.5 | - |
meso-2,6-diaminoheptanedioate | mutant K159R, pH not specified in the publication, temperature not specified in the publication | Symbiobacterium thermophilum | |
| 36.05 | - |
meso-2,6-diaminoheptanedioate | mutant V68M, pH not specified in the publication, temperature not specified in the publication | Symbiobacterium thermophilum | |
| 36.27 | - |
meso-2,6-diaminoheptanedioate | mutant V14L, pH not specified in the publication, temperature not specified in the publication | Symbiobacterium thermophilum | |
| 39.8 | - |
meso-2,6-diaminoheptanedioate | wild-type, pH not specified in the publication, temperature not specified in the publication | Symbiobacterium thermophilum | |
| 48.79 | - |
meso-2,6-diaminoheptanedioate | mutant P69G, pH not specified in the publication, temperature not specified in the publication | Symbiobacterium thermophilum | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.16 | - |
pyruvate | mutant V14L, pH not specified in the publication, temperature not specified in the publication | Symbiobacterium thermophilum | |
| 0.18 | - |
pyruvate | mutant P69G, pH not specified in the publication, temperature not specified in the publication | Symbiobacterium thermophilum | |
| 0.74 | - |
pyruvate | mutant S90T, pH not specified in the publication, temperature not specified in the publication | Symbiobacterium thermophilum | |
| 0.78 | - |
pyruvate | mutant T70S, pH not specified in the publication, temperature not specified in the publication | Symbiobacterium thermophilum | |
| 0.86 | - |
pyruvate | wild-type, pH not specified in the publication, temperature not specified in the publication | Symbiobacterium thermophilum | |
| 0.86 | - |
pyruvate | mutant V68M, pH not specified in the publication, temperature not specified in the publication | Symbiobacterium thermophilum | |
| 1.06 | - |
pyruvate | mutant K159R, pH not specified in the publication, temperature not specified in the publication | Symbiobacterium thermophilum | |
| 1.15 | - |
meso-2,6-diaminoheptanedioate | mutant V156D, pH not specified in the publication, temperature not specified in the publication | Symbiobacterium thermophilum | |
| 32.4 | - |
meso-2,6-diaminoheptanedioate | mutant S90T, pH not specified in the publication, temperature not specified in the publication | Symbiobacterium thermophilum | |
| 35.5 | - |
meso-2,6-diaminoheptanedioate | mutant V14L, pH not specified in the publication, temperature not specified in the publication | Symbiobacterium thermophilum | |
| 69.7 | - |
meso-2,6-diaminoheptanedioate | mutant V68M, pH not specified in the publication, temperature not specified in the publication | Symbiobacterium thermophilum | |
| 113.3 | - |
meso-2,6-diaminoheptanedioate | mutant P69G, pH not specified in the publication, temperature not specified in the publication | Symbiobacterium thermophilum | |
| 117.2 | - |
meso-2,6-diaminoheptanedioate | mutant K159R, pH not specified in the publication, temperature not specified in the publication | Symbiobacterium thermophilum | |
| 130 | - |
meso-2,6-diaminoheptanedioate | mutant T70S, pH not specified in the publication, temperature not specified in the publication | Symbiobacterium thermophilum | |
| 148.7 | - |
meso-2,6-diaminoheptanedioate | wild-type, pH not specified in the publication, temperature not specified in the publication | Symbiobacterium thermophilum | |
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