Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Symbiobacterium thermophilum |
Protein Variants | Comment | Organism |
---|---|---|
R35S | the mutant shows reduced catalytic efficiency with meso-2,6-diaminoheptanedioate and NADP+ compared to the wild type enzyme | Symbiobacterium thermophilum |
R71A | the mutant shows about wild type catalytic efficiency with meso-2,6-diaminoheptanedioate and increased catalytic efficiency with NADP+ compared to the wild type enzyme | Symbiobacterium thermophilum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1 | - |
NADPH | wild type enzyme, at pH 9.6 and 25°C | Symbiobacterium thermophilum | |
0.18 | - |
NADPH | mutant enzyme R71A, at pH 9.6 and 25°C | Symbiobacterium thermophilum | |
0.35 | - |
NADPH | mutant enzyme R35S, at pH 9.6 and 25°C | Symbiobacterium thermophilum | |
0.35 | - |
NADP+ | wild type enzyme, at pH 9.6 and 25°C | Symbiobacterium thermophilum | |
0.39 | - |
meso-2,6-diaminoheptanedioate | mutant enzyme R35S, at pH 9.6 and 25°C | Symbiobacterium thermophilum | |
0.39 | - |
NADP+ | mutant enzyme R71A, at pH 9.6 and 25°C | Symbiobacterium thermophilum | |
0.41 | - |
meso-2,6-diaminoheptanedioate | wild type enzyme, at pH 9.6 and 25°C | Symbiobacterium thermophilum | |
0.42 | - |
meso-2,6-diaminoheptanedioate | mutant enzyme R71A, at pH 9.6 and 25°C | Symbiobacterium thermophilum | |
0.46 | - |
NADP+ | mutant enzyme R35S, at pH 9.6 and 25°C | Symbiobacterium thermophilum | |
7.45 | - |
pyruvate | mutant enzyme R35S, at pH 8.6 and 25°C | Symbiobacterium thermophilum | |
16.31 | - |
pyruvate | wild type enzyme, at pH 8.6 and 25°C | Symbiobacterium thermophilum | |
21.77 | - |
pyruvate | mutant enzyme R71A, at pH 8.6 and 25°C | Symbiobacterium thermophilum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
meso-2,6-diaminoheptanedioate + H2O + NADP+ | Symbiobacterium thermophilum | - |
L-2-amino-6-oxoheptanedioate + NH3 + NADPH + H+ | - |
r | |
meso-2,6-diaminoheptanedioate + H2O + NADP+ | Symbiobacterium thermophilum IAM14863 | - |
L-2-amino-6-oxoheptanedioate + NH3 + NADPH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Symbiobacterium thermophilum | - |
- |
- |
Symbiobacterium thermophilum IAM14863 | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxosuccinic acid + NH3 + NADPH + H+ | low activity | Symbiobacterium thermophilum | ? | - |
? | |
2-oxosuccinic acid + NH3 + NADPH + H+ | low activity | Symbiobacterium thermophilum IAM14863 | ? | - |
? | |
3-methyl-2-oxobutanoate + NH3 + NADPH + H+ | low activity | Symbiobacterium thermophilum | ? | - |
? | |
3-methyl-2-oxobutanoate + NH3 + NADPH + H+ | low activity | Symbiobacterium thermophilum IAM14863 | ? | - |
? | |
4-methyl-2-oxopentanoic acid + NH3 + NADPH + H+ | low activity | Symbiobacterium thermophilum | ? | - |
? | |
meso-2,6-diaminoheptanedioate + H2O + NADP+ | - |
Symbiobacterium thermophilum | L-2-amino-6-oxoheptanedioate + NH3 + NADPH + H+ | - |
r | |
meso-2,6-diaminoheptanedioate + H2O + NADP+ | highest activity | Symbiobacterium thermophilum | L-2-amino-6-oxoheptanedioate + NH3 + NADPH + H+ | - |
r | |
meso-2,6-diaminoheptanedioate + H2O + NADP+ | - |
Symbiobacterium thermophilum IAM14863 | L-2-amino-6-oxoheptanedioate + NH3 + NADPH + H+ | - |
r | |
meso-2,6-diaminoheptanedioate + H2O + NADP+ | highest activity | Symbiobacterium thermophilum IAM14863 | L-2-amino-6-oxoheptanedioate + NH3 + NADPH + H+ | - |
r | |
pyruvate + NADPH | - |
Symbiobacterium thermophilum | ? | - |
? | |
pyruvate + NADPH | - |
Symbiobacterium thermophilum IAM14863 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
meso-DAPDH | - |
Symbiobacterium thermophilum |
meso-diaminopimelate dehydrogenase | - |
Symbiobacterium thermophilum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.92 | - |
NADPH | mutant enzyme R35S, at pH 9.6 and 25°C | Symbiobacterium thermophilum | |
2.96 | - |
pyruvate | mutant enzyme R35S, at pH 8.6 and 25°C | Symbiobacterium thermophilum | |
3.31 | - |
pyruvate | mutant enzyme R71A, at pH 8.6 and 25°C | Symbiobacterium thermophilum | |
4.17 | - |
NADPH | mutant enzyme R71A, at pH 9.6 and 25°C | Symbiobacterium thermophilum | |
6.97 | - |
NADPH | wild type enzyme, at pH 9.6 and 25°C | Symbiobacterium thermophilum | |
7 | - |
pyruvate | wild type enzyme, at pH 8.6 and 25°C | Symbiobacterium thermophilum | |
17.91 | - |
NADP+ | mutant enzyme R35S, at pH 9.6 and 25°C | Symbiobacterium thermophilum | |
25.47 | - |
NADP+ | wild type enzyme, at pH 9.6 and 25°C | Symbiobacterium thermophilum | |
29.44 | - |
meso-2,6-diaminoheptanedioate | mutant enzyme R35S, at pH 9.6 and 25°C | Symbiobacterium thermophilum | |
40.46 | - |
meso-2,6-diaminoheptanedioate | mutant enzyme R71A, at pH 9.6 and 25°C | Symbiobacterium thermophilum | |
40.47 | - |
meso-2,6-diaminoheptanedioate | wild type enzyme, at pH 9.6 and 25°C | Symbiobacterium thermophilum | |
58.16 | - |
NADP+ | mutant enzyme R71A, at pH 9.6 and 25°C | Symbiobacterium thermophilum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Symbiobacterium thermophilum |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.15 | - |
pyruvate | mutant enzyme R71A, at pH 8.6 and 25°C | Symbiobacterium thermophilum | |
0.4 | - |
pyruvate | mutant enzyme R35S, at pH 8.6 and 25°C | Symbiobacterium thermophilum | |
0.43 | - |
pyruvate | wild type enzyme, at pH 8.6 and 25°C | Symbiobacterium thermophilum | |
8.32 | - |
NADPH | mutant enzyme R35S, at pH 9.6 and 25°C | Symbiobacterium thermophilum | |
23.49 | - |
NADPH | mutant enzyme R71A, at pH 9.6 and 25°C | Symbiobacterium thermophilum | |
39.02 | - |
NADP+ | mutant enzyme R35S, at pH 9.6 and 25°C | Symbiobacterium thermophilum | |
69.29 | - |
NADPH | wild type enzyme, at pH 9.6 and 25°C | Symbiobacterium thermophilum | |
73.83 | - |
NADP+ | wild type enzyme, at pH 9.6 and 25°C | Symbiobacterium thermophilum | |
75.07 | - |
meso-2,6-diaminoheptanedioate | mutant enzyme R35S, at pH 9.6 and 25°C | Symbiobacterium thermophilum | |
96.54 | - |
meso-2,6-diaminoheptanedioate | mutant enzyme R71A, at pH 9.6 and 25°C | Symbiobacterium thermophilum | |
98.48 | - |
meso-2,6-diaminoheptanedioate | wild type enzyme, at pH 9.6 and 25°C | Symbiobacterium thermophilum | |
148.06 | - |
NADP+ | mutant enzyme R71A, at pH 9.6 and 25°C | Symbiobacterium thermophilum |